1989
DOI: 10.1128/jb.171.5.2842-2849.1989
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Biochemical characterization of avirulent Agrobacterium tumefaciens chvA mutants: synthesis and excretion of beta-(1-2)glucan

Abstract: The chvA gene product of Agrobacterium tumefaciens is required for virulence and attachment of bacteria to plant cells. Three chvA mutants were studied. In vivo, they were defective in the synthesis, accumulation, and secretion of 0-(1-2)glucan; however, the 235-kilodalton (kDa) protein known to be involved in the synthesis of P-(1-2)glucan (A. Zorreguieta and R. Ugalde, J. Bacteriol. 167:947-951, 1986) was present and active in vitro.Two molecular forms of cyclic 0-(1-2)glucan, designated types I and II, were… Show more

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Cited by 60 publications
(48 citation statements)
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“…Cyclic ␤-1,2-glucan recovered from Bio-Gel P4 columns was successively treated with HCl (10 mM) at 100°C for 90 min and with NaOH (0.1 N) at 37°C for 30 min as previously described for removing polysaccharide nonsugar substituents (25).…”
Section: Methodsmentioning
confidence: 99%
“…Cyclic ␤-1,2-glucan recovered from Bio-Gel P4 columns was successively treated with HCl (10 mM) at 100°C for 90 min and with NaOH (0.1 N) at 37°C for 30 min as previously described for removing polysaccharide nonsugar substituents (25).…”
Section: Methodsmentioning
confidence: 99%
“…The first is a rather weak and reversible binding step that may involve a variety of bacterial polysaccharides. The products of the ndvA and ndvB genes in Sinorhizobium meliloti and of the homologous chvA and chvB genes in A. tumefaciens are involved in the synthesis of a cyclic glucan (64,103,236,468), which could act as an adhesin via gelling interactions with host polysaccharides or could interact with plant lectins (215). Mutations in the chv genes reduced the binding of the agrobacteria to cultured cells and abolished tumorigenesis (117,118,395).…”
Section: Binding To Host Surfacesmentioning
confidence: 99%
“…It can also be speculated that the localization of the enzyme in the vicinity of the membrane is important for the transport of its product (cyclic glucan) into the periplasmic space by the action of the cyclic glucan transporter Cgt (which is also an integral inner membrane protein) (38). Experimental evidence has suggested that in Agrobacterium tumefaciens the cyclic glucan synthase (ChvB) interacts with the cyclic glucan transporter (ChvA) (21). Sequence analysis of the amino terminus of Cgs (residues 1 to 400) with the 3D-pssm program (Imperial College of Science, Technology & Medicine [http://www.sbg.bio.ic.ac.uk]) (22) revealed a predicted fold similar to the fold of the sigma 70 subunit fragment of E. coli RNA polymerase, the peroxisomal targeting signal-1 receptor, and the tetratricopeptide repeat motif.…”
Section: Discussionmentioning
confidence: 99%