Biochemical characterization of synthesized fisetin glucoside by dextransucrase from Leuconostoc mesenteroides NRRL B-1299CB4 with enhanced water solubility

Moon, Yedam; Kim, Hayeong; Kang, Choon Gil; Park, Chanho; Kim, Seung Wook; Kim, Doman

doi:10.1016/j.enzmictec.2022.110111

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…FIS must provide high-resolution control of spatial tissue and effective release of the drug in a controlled manner due to characteristics (e.g., low water solubility and low bioavailability), [31,32] such that it is imperative to select an appropriate drug carrier (e.g., chitosan hydrogel). Hydrogels containing FIS were evaluated as drug delivery vehicles for FIS at 37 °C and examined for their cumulative release profiles from PBS (pH 7.3).…”

Section: Materials Characterizationmentioning

confidence: 99%

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Fang,

Xu,

Jin

et al. 2024

Macromolecular Bioscience

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Peripheral nerve injuries represent a prevalent and severe category of damage resulting from traumatic incidents. Predominantly, the deficiency in nerve regeneration can be ascribed to enduring inflammatory reactions, hence imposing substantial clinical implications for patients. Fisetin, a flavonoid derived from plants, is naturally present in an array of vegetables and fruits, including strawberries, apples, onions, and cucumbers. It exhibits immunomodulatory properties through the reduction of inflammation and oxidative stress. In the present research, a nerve defect was addressed for the first time utilizing a scaffold primed for controlled fisetin release. In this regard, fisetin‐loaded chitosan hydrogels were incorporated into the lumen of polycaprolactone (PCL) nerve guide conduits (NGCs). The hydrogel maintained a steady release of an appropriate fisetin dosage. The study outcomes indicated that the fisetin/chitosan/polycaprolactone (FIS/CS/PCL) NGCs amplified Schwann cell proliferation and neural expression, curtailed oxidative stress, alleviated inflammation, and improved functions, electrophysiological properties, and morphology. This pioneering scaffold has potential to contribute significantly to the field of neuroengineering.This article is protected by copyright. All rights reserved

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Section: Materials Characterizationmentioning

confidence: 99%

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Fang,

Xu,

Jin

et al. 2024

Macromolecular Bioscience

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“…However, the anti-inflammatory effect of fisetin glucoside was enhanced via the inhibition of NO release by 92.3% compared to fisetin (81.4%). Anti-lipid accumulation activity in mouse 3T3-L1 cells was evaluated, and the results showed similar effects for both compounds [ 133 ].…”

Section: Biotechnological Modifications and Combination With Biotechn...mentioning

confidence: 99%

Fisetin—In Search of Better Bioavailability—From Macro to Nano Modifications: A Review

Szymczak,

Cielecka-Piontek

2023

IJMS

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As secondary plant metabolites, polyphenols are abundant in fruits and vegetables. They are in high demand because of their many health benefits. However, their low bioavailability makes them complex compounds to use for therapeutic purposes. Due to the limited solubility of phytocompounds, dietary supplements made from them may only be partially effective. Such molecules include fisetin, found in strawberries, and have shown great promise in treating Alzheimer’s disease and cancer. Unfortunately, because of their limited water solubility, low absorption, and poor bioavailability, the assistance of nanotechnology is required to allow them to fulfil their potential fully. Here, we provide evidence that nanodelivery methods and structure modifications can improve fisetin bioavailability, which is linked to improvements in therapeutic efficacy. An open question remains as to which nanocarrier should be chosen to meet the abovementioned requirements and be able to enhance fisetin’s therapeutic potential to treat a particular disease.

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“…c,d) 1 H-HMR spectra of the glucan and the oligoglucan. (e,f) 13 C-HMR spectra of the glucan and the oligoglucan.…”

Section: Nuclear Magnetic Resonance (Nmr) Analysismentioning

confidence: 99%

“…The dried glucan and oligoglucan samples were weighed (30 mg each) and dissolved in 0.5 mL of heavy water in an NMR tube (Nuclear Magnetic Resonance Spectrometer, Bruker Avance NEO 600, Bruker Corporation, Billerica, MA, USA). 1 H-NMR and 13 C-NMR spectra were scanned at 25 • C with resonance frequencies of 600 MHz and 150 MHz, respectively [44].…”

Section: Nuclear Magnetic Resonance (Nmr)mentioning

confidence: 99%

“…L. citreum KM20-glucansucrase-catalyzed steviol glycoside transglycosylation products can serve as commercial sweeteners with improved stability [ 12 ]. Fisetin-4′-O-α-D-glucopyranoside (FST-G1) with glucansucrase from L. mesenteroides NRRL B-1299CB4 exhibited greater water solubility and anti-inflammatory effects [ 13 ]. There are various types of glucan acceptors; of these, maltose is the most effective for glucan and oligosaccharide synthesis [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function

Gu,

Jiao,

Wang

et al. 2023

IJMS

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This study explored the physicochemical properties and prebiotic activities of glucans and oligoglucans. Oligoglucans were obtained through the fermentation of Leuconostoc mesenteroides CICC6055 and the glucansucrase of strain CICC6055, while glucans were obtained only through fermentation. Thin-layer chromatography and high-performance liquid chromatography identified enzymatically synthesized oligoglucans with a higher yield. Differential scanning calorimetry and derivative thermogravimetry analyses revealed the heat resistance of the glucans and oligoglucans at 280–300 °C. Fourier transform-infrared spectroscopy and nuclear magnetic resonance analyses demonstrated that their main chains were linked with α-1,6-glycosidic bonds accompanied by glucose residue branching. In vitro fermentation experiments demonstrated that they not only improved the contents of short-chain fatty acids but also raised the abundance of predominant flora, such as Bacteroides, Firmicutes, Verrucomicrobia, and Proteobacteria. These results implicate glucansucrase as an efficacious tool for the enzyme synthesis of oligoglucans. Furthermore, both polysaccharides with different degrees of polymerization may be beneficial in maintaining a healthy human gut.

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Biochemical characterization of synthesized fisetin glucoside by dextransucrase from Leuconostoc mesenteroides NRRL B-1299CB4 with enhanced water solubility

Cited by 7 publications

References 35 publications

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Fisetin—In Search of Better Bioavailability—From Macro to Nano Modifications: A Review

Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function

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