Biochemical Characterization of Thermostable Carboxymethyl Cellulase and β-Glucosidase from Aspergillus fumigatus JCM 10253

Saroj, Paramjeet; Manasa, P; Narasimhulu, Korrapati

doi:10.1007/s12010-022-03839-2

Cited by 27 publications

(11 citation statements)

References 116 publications

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“…such as A . versicolor and A. fumigatus JCM 10253 with molecular mass of 100 kDa [ 16 ] and 125 kDa, respectively [ 17 ]. However, BGL with a similar molecular mass was also obtained by Pal [ 25 ] from Termitomyces clypeatus, another filamentous fungus.…”

Section: Resultsmentioning

confidence: 99%

“…Concurring with these results, unpurified BGL from a different B. bassiana strain was previously shown to be active under moderately acidic conditions [ 24 ], in addition, most fungal BGLs have been reported to be active at pH 4.0–6.0. Recent examples include the BGL from Aspergillus fumigatus [ 26 ] and Neofusicoccum parvum [ 27 ]. The BGL in this study retained >60 residual activity after 4 h incubation at pH 4.0, 5.0, and 6.0, respectively ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Borgi [ 24 ] had previously recorded an optimal temperature of 55 °C for crude BGL preparation from another B. bassiana strain. Similarly, other mesophilic fungi besides B. bassiana have been recorded to produce BGL with optimum activity between 40 and 60C°, such as Aspergillus flavus [ 28 ], A. fumigatus JCM 10253 [ 17 ], and Aspergillus sp. DHE7 [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…The inhibitory or stimulatory effects of the different organic solvents, viz ., acetone, benzene, ethanol, butanol, hexane, methanol, and toluene on BGL activity were assessed. The enzyme was incubated for 1 h at room temperature in solutions containing each solvent (10% v/v) with continuous agitation at 120 rpm [ 17 ]. Subsequently, aliquots were removed, and the relative activity was measured according to the standard enzyme assay mentioned in section 2.4 .…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana

Magwaza,

Amobonye,

Bhagwat

et al. 2024

Heliyon

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana

Magwaza,

Amobonye,

Bhagwat

et al. 2024

Heliyon

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“…and their consortium were also reported to be able to live in extreme environment of polluted soil and yielded long hydrocarbon-decomposing enzymes (Novianty et al 2021). Saroj et al (2022) reported that the cellulase from A. fumigatus JCM 10253 exhibited optimum activity at 50°C, which the purified cellulases showed significant tolerance against various metal ions, denaturing agents, and organic solvents. Another report found that the crude cellulase secreted by A. fumigatus Z5 was further applied to hydrolyze pretreated corn stover and the enzymatic hydrolysate was successfully used as a substrate for ethanol production by Saccharomyces cerevisiae (Liu et al 2011).…”

Section: Molecular Identificationmentioning

confidence: 99%

Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia

et al. 2022

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Abstract. Saryono, Novianty R, Suraya N, Piska F, Devi S, Pratiwi NW, Ardhi A. 2022. Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia. Biodiversitas 23: 1457-1465. Thermostable cellulolytic enzymes have become a subject of interest in industrial processes due to their ability to degrade cellulosic polysaccharides at elevated temperatures produced by microorganisms such as fungi and bacteria. In the present study, cellulase-producing thermophilic fungi were isolated and identified from Sungai Pinang hot spring, Riau Province, Indonesia. Morphological identification was carried out by macroscopic and microscopic observations. The ability of the thermophilic fungi to produce cellulase was determined using the clear zone test on carboxymethyl cellulose medium with a congo red staining. Isolate with the highest activity was identified molecularly using universal primers ITS1F and ITS4R. The results showed 19 isolates were morphologically identified as Aspergillus sp. and Penicillium sp. Based on qualitative testing, 3 of the19 isolates showed cellulase activity. The isolate performing the most considerable cellulase was LBKURCC293, which was identified as Aspergillus fumigatus with a cellulase activity of 2.6 x 10-2 IU/mL and a specific cellulase activity 8.0 x 10-3 IU/mg protein after 96 days of incubation.

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A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

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The presence of stone cells in pears is recognized as a problem for the pear processing industry. Bacillus and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study Lactoplantibacillus plantarum JYLP‐326 was used in single culture or in the mixed culture with Saccharomyces cerevisiae Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed‐culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β‐glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed‐culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed‐culture fermentation using the above‐mentioned strains could be exploited by the pear processing industry to degrade stone cells.

show abstract

Biochemical Characterization of Thermostable Carboxymethyl Cellulase and β-Glucosidase from Aspergillus fumigatus JCM 10253

Cited by 27 publications

References 116 publications

Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana

Biochemical and in silico structural properties of a thermo-acid stable β-glucosidase from Beauveria bassiana

Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

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