Biochemical comparison of plaque fluid on tooth and acrylic surfaces during a sucrose challenge

Rankine, C.A.N.; Smith, Sidney L.; Schneider, P; Gardiner, Diana

doi:10.1016/s0003-9969(96)00029-5

Cited by 6 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The explanation for this is not clear. While there is evidence that enamel contributes to plaque calcium concen-trations upon exposure to sucrose [Rankine et al, 1996;Tanaka and Margolis, 1999], saliva is probably the major source of the calcium acquired by plaque since it is supersaturated with several calcium salts [Larsen, 1975;Carey et al, 1986;Pearce, 1991;Shellis and Duckworth, 1994;Larsen and Pearce, 2003]. However, while plaque calcium concentrations vary widely, salivary calcium concentrations fall within a narrow range (table 1).…”

Section: Discussionmentioning

confidence: 99%

Plaque Fluoride Concentrations in a Community without Water Fluoridation: Effects of Calcium and Use of a Fluoride or Placebo Dentifrice

et al. 2005

View full text Add to dashboard Cite

The results of a recent study by Whitford et al. [Caries Res 2002;36:256–265] with subjects whose drinking water was fluoridated led to two major conclusions: (1) Compared to the use of a placebo dentifrice, plaque fluoride concentrations ([F]) throughout much of the day are not significantly increased by the use of an F dentifrice but (2) they are positively related to plaque [Ca] (p = 0.0001). The present double-blind, double-crossover study with 16 subjects used the same protocol and was done to: (1) determine the effects of the use of an F dentifrice on salivary and plaque [F] in a community without water fluoridation and (2) further examine the relationship between plaque [Ca] and [F]. Following the use of an F dentifrice or placebo for one week, whole saliva and plaque were collected 1.0 and 12 h after the last use of the products. The study was repeated to include rinsing with a 20 mmol/l CaCl₂ solution immediately before the use of the dentifrices. The CaCl₂ rinse had only minor effects on salivary [Ca] and [F] and none on the plaque concentrations. Unlike the results found in the fluoridated community, all salivary and plaque [F] associated with the use of the F dentifrice were significantly higher than those associated with the use of the placebo. The results suggest that the cariostatic effectiveness of an F dentifrice should be greater in areas without water fluoridation. As noted previously, plaque [F] were positively related to plaque [Ca] (p = 0.0001).

show abstract

Section: Discussionmentioning

confidence: 99%

Plaque Fluoride Concentrations in a Community without Water Fluoridation: Effects of Calcium and Use of a Fluoride or Placebo Dentifrice

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Colonisation and growth of these micro-organisms also takes place on artificial surfaces such as dental materials, but with somewhat different properties [10,30], from where they may spread to the teeth or mucosal areas. Quirynen et al [29] found a significant difference in plaque composition on fluorethylene propylene-coated surfaces compared to titanium.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of salivary components in the pellicle on PMMA denture base material

Göcke¹,

Gerath

Schwanewede

2002

Clinical Oral Investigations

View full text Add to dashboard Cite

The formation of a salivary pellicle is a protective mechanism of the body for all surfaces in the oral cavity. The nature of the substrate may influence the composition of the pellicle. The aim of this study was to investigate the quantitative composition and individual variation of the salivary pellicle formed on denture base material (PMMA). Cylindrical specimens of PMMA were carried in the mouth and then desorbed with a 0.5-M sodium chloride solution. The solution was analysed for total protein, alpha-amylase, total proteases, protease inhibitors, secretory immunoglobulin A, immunoglobulin G, peroxidases, thiocyanate, lysozyme, and calcium content. All investigated salivary components could be found unequivocally in the desorption solution, indicating that a salivary pellicle had formed on the surface of the PMMA. Large coefficients of variation indicate large individual variations in the adsorbed amounts. The data also point to large intraindividual variations for the bound salivary components. Only the protease inhibitors revealed a strong positive correlation of the bound activity to the salivary activity. It is hypothesised that differences in the bound amounts of antimicrobial components might influence the microbial colonisation of denture bases and that protease inhibitors could be meaningful for the spread of the yeast Candida albicans from denture base material to the oral mucosa and thus might be an explanation for different susceptibility to denture base stomatitis.

show abstract

“…We thus hypothesize that the increased Ca concentration in the biofilm fluid shortly after a sugar challenge would be partially accounted for by the dissolution of biofilm reservoirs, but the continuous elevated Ca concentrations observed by others on enamel and dentine after longer periods [Margolis and Moreno, 1992;Margolis et al, 1993a] would be maintained at the expense of minerals dissolving from the tooth substrate. This could explain why Rankine et al [1996] could not find an increase in the concentration of Ca in the biofilm formed on acrylic 15 min after exposure to sugar, while for enamel this could be seen. However, this hypothesis needs to be confirmed by additional studies.…”

Section: Resultsmentioning

confidence: 91%

“…The role of biofilm mineral reservoirs as a source of ions to the fluid was studied by Rankine et al [1996], who did not find, after sugar exposure, a significant increase in the concentration of ions in the fluid of biofilm formed on an inert substrate, such as acrylic. However, the analysis was made 15 min after the sugar exposure, when the pH was rising and equilibrium could have been reached again between the fluid and biofilm reservoirs.…”

mentioning

confidence: 99%

Mineral Ions in the Fluid of Biofilms Formed on Enamel and Dentine Shortly after Sugar Challenge

Correia

Tenuta²,

Cury³

et al. 2012

Caries Res

View full text Add to dashboard Cite

To test the effect of distinct solubilities of dentine and enamel on mineral ion concentration in the biofilm fluid during a sugar-induced pH drop, dental biofilms were formed in situ for 4 days on acrylic (control), dentine or enamel. On the 5th day, they were treated with water (control) or 20% glucose and collected 5 min later. Significantly lower pH values and higher calcium concentrations were found in the biofilm fluid after glucose exposure, without significant differences among the three substrates. During pH drop, biofilm reservoirs release calcium to the fluid, masking the differential solubility between enamel and dentine.

show abstract

Biochemical comparison of plaque fluid on tooth and acrylic surfaces during a sucrose challenge

Cited by 6 publications

References 11 publications

Plaque Fluoride Concentrations in a Community without Water Fluoridation: Effects of Calcium and Use of a Fluoride or Placebo Dentifrice

Plaque Fluoride Concentrations in a Community without Water Fluoridation: Effects of Calcium and Use of a Fluoride or Placebo Dentifrice

Quantitative determination of salivary components in the pellicle on PMMA denture base material

Mineral Ions in the Fluid of Biofilms Formed on Enamel and Dentine Shortly after Sugar Challenge

Contact Info

Product

Resources

About