Biochemical degradation pathway of dimethoate by Paracoccus sp. Lgjj-3 isolated from treatment wastewater

Li, Rong; Zheng, Jingwei; Wang, Rong; Song, Yongwei; Chen, Qiming; Yang, Xia; Li, Shunpeng; Jiang, Jiandong

doi:10.1016/j.ibiod.2009.10.007

Cited by 77 publications

(33 citation statements)

References 15 publications

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“…The DNA of strain DFS35-4 was extracted by high-salt-concentration precipitation (Miller et al 1988). The 16S rRNA gene was amplified by a previously described PCR method (Li et al 2010). The nucleotide sequence coding for the 16S rRNA of strain DFS35-4 (1,410 bp) was deposited in the GenBank database under the accession number FJ544978.…”

Section: Strain Isolation and Characterizationmentioning

confidence: 99%

“…Twenty milliliter samples of cultures were collected in 5 days and extracted with an equal volume of dichloromethane. The residual aqueous phase was extracted with an equal volume of ethyl acetate, acidified to pH 2.0, and extracted with an equal volume of ethyl acetate (Li et al 2010). Extracts from dichloromethane and ethyl acetate were mixed.…”

Section: Identification Of the Metabolites During Buprofezin Biodegramentioning

confidence: 99%

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Isolation of a buprofezin co-metabolizing strain of Pseudomonas sp. DFS35-4 and identification of the buprofezin transformation pathway

Chen

Liu

et al. 2011

Biodegradation

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Buprofezin is a widely used insecticide that has caused environmental pollution in many areas. However, biodegradation of buprofezin by pure cultures has not been extensively studied, and the transformation pathway of buprofezin remains unclear. In this paper, a buprofezin co-metabolizing strain of DFS35-4 was isolated from a buprofezin-polluted soil in China. Strain DFS35-4 was preliminarily identified as Pseudomonas sp. based on its morphological, physiological, and biochemical properties, as well as 16S rRNA gene analysis. In the presence of 2.0 g l(-1) sodium citrate, strain DFS35-4 degraded over 70% of 50 mg l(-1) buprofezin in 3 days. Strain DFS35-4 efficiently degraded buprofezin in the pH range of 5.0-10.0 and at temperatures between 20 and 30°C. Three metabolites, 2-imino-5-phenyl-3-(propan-2-yl)-1,3,5-thiadiazinan-4-one, 2-imino-5-phenyl-1,3,5-thiadiazinan-4-one, and methyl(phenyl) carbamic acid, were identified during the degradation of buprofezin using gas chromatography-mass spectrometry (GC-MS) and tandem mass spectrometry (MS/MS). A partial transformation pathway of buprofezin in Pseudomonas sp. DFS35-4 was proposed based on these metabolites.

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Section: Strain Isolation and Characterizationmentioning

confidence: 99%

Section: Identification Of the Metabolites During Buprofezin Biodegramentioning

confidence: 99%

Isolation of a buprofezin co-metabolizing strain of Pseudomonas sp. DFS35-4 and identification of the buprofezin transformation pathway

Chen

Liu

et al. 2011

Biodegradation

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“…Bacterial strains that are able to degrade amide pesticides (carbofuran, carbaryl, acetochlor, carbendazim, dimethoate, etc.) have been isolated, and their microbial metabolic pathways have also been elucidated (12,22,25,32,43). To date, the sequence information for the following amide pesticide-hydrolyzing enzymes is available: carbaryl hydrolases CahA and CehA (11, 12), phenmedipham hydrolase PCD (26), carbofuran hydrolase Mcd (32), carbendazimhydrolyzing esterase MheI (25), propanil-hydrolyzing amidases Amq, PamH, and PrpH (19,28,42), and phenylurea hydrolases PuhA and LibA (2, 34).…”

mentioning

confidence: 99%

Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides

Zhang

Yin

Hang

et al. 2012

Appl Environ Microbiol

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bThe bacterial isolate Paracoccus sp. strain FLN-7 hydrolyzes amide pesticides such as diflubenzuron, propanil, chlorpropham, and dimethoate through amide bond cleavage. A gene, ampA, encoding a novel arylamidase that catalyzes the amide bond cleavage in the amide pesticides was cloned from the strain. ampA contains a 1,395-bp open reading frame that encodes a 465-aminoacid protein. AmpA was expressed in Escherichia coli BL21 and homogenously purified using Ni-nitrilotriacetic acid affinity chromatography. AmpA is a homodimer with an isoelectric point of 5.4. AmpA displays maximum enzymatic activity at 40°C and a pH of between 7.5 and 8.0, and it is very stable at pHs ranging from 5.5 to 10.0 and at temperatures up to 50°C. AmpA efficiently hydrolyzes a variety of secondary amine compounds such as propanil, 4-acetaminophenol, propham, chlorpropham, dimethoate, and omethoate. The most suitable substrate is propanil, with K m and k cat values of 29.5 M and 49.2 s ؊1 , respectively. The benzoylurea insecticides (diflubenzuron and hexaflumuron) are also hydrolyzed but at low efficiencies. No cofactor is needed for the hydrolysis activity. AmpA shares low identities with reported arylamidases (less than 23%), forms a distinct lineage from closely related arylamidases in the phylogenetic tree, and has different biochemical characteristics and catalytic kinetics with related arylamidases. The results in the present study suggest that AmpA is a good candidate for the study of the mechanism for amide pesticide hydrolysis, genetic engineering of amide herbicide-resistant crops, and bioremediation of amide pesticide-contaminated environments.A variety of amide compounds are used as pesticides to control insects, pathogens, and weeds in agriculture. These compounds include benzoylurea insecticides (hexaflumuron, diflubenzuron, etc.), organophosphate insecticides with an amide group (dimethoate, omethoate, etc.), carbamate insecticides or herbicides (carbofuran, carbaryl, propham, chlorpropham, etc.), benzimidazole fungicides (carbendazim, benomyl, etc.), acetanilide herbicides (acetochlor, butachlor, propanil, etc.), substituted phenylurea herbicides (diuron, linuron, etc.), and sulfonylurea herbicides (chlorsulfuron, metsulfuron-methyl, etc.) (33). However, the widespread use of amide pesticides has resulted in the contamination of the environment and agricultural products. Moreover, many amide pesticides are hazardous to human health and may damage crops when used improperly (7,8,33). Thus, the removal of amide pesticides from the environment and agricultural products is of paramount importance.Microorganisms play a significant role in the degradation or detoxification of amide pesticides in the environment. Bacterial strains that are able to degrade amide pesticides (carbofuran, carbaryl, acetochlor, carbendazim, dimethoate, etc.) have been isolated, and their microbial metabolic pathways have also been elucidated (12,22,25,32,43). To date, the sequence information for the following amide pesticide-hydrolyzing enzymes is...

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“…Similary, by using Paracoccus sp. Lgjj-3, the biodegradation of dimethoate with detailed biochemical pathway is also reported (Li et al, 2010). Till date number of bacterial species has been reported for dichlorovos degradation.…”

Section: Bioremediation Of Organophosphorousmentioning

confidence: 98%

Microbial degradation of pesticide: A review

Parte¹,

Mohekar²,

Kharat³

2017

Afr. J. Microbiol. Res.

165

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Biochemical degradation pathway of dimethoate by Paracoccus sp. Lgjj-3 isolated from treatment wastewater

Cited by 77 publications

References 15 publications

Isolation of a buprofezin co-metabolizing strain of Pseudomonas sp. DFS35-4 and identification of the buprofezin transformation pathway

Isolation of a buprofezin co-metabolizing strain of Pseudomonas sp. DFS35-4 and identification of the buprofezin transformation pathway

Cloning of a Novel Arylamidase Gene from Paracoccus sp. Strain FLN-7 That Hydrolyzes Amide Pesticides

Microbial degradation of pesticide: A review

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