Biochemical Engineering Approaches for Increasing Viability and Functionality of Probiotic Bacteria

Nguyen, Huu Thanh; Truong, Dieu‐Hien; Kouhounde, Sonagnon Hermann Serge; Ly, Sarann; Razafindralambo, Hary; Delvigne, Frank

doi:10.3390/ijms17060867

Cited by 46 publications

(20 citation statements)

References 131 publications

(200 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nowadays, probiotics are among the most investigated research topics, 1 owing to their beneficial effects on human, 2 animal, 3 and plant 4 species. These living microorganisms are able to promote the host health by playing both preventive and therapeutic roles against numerous diseases, when they are used in adequate amounts.…”

Section: Introductionmentioning

confidence: 99%

Bulk and Surface Wettability Characteristics of Probiotic Powders in Their Compressed Disc and Packed-Bed Column Forms

et al. 2020

View full text Add to dashboard Cite

Most probiotic-based products are available in powder particles under different solid-state forms. Such diversity can affect the probiotic stability, viability, and performance at different stages of processing, storage, and use. Here, we apply complementary physical chemistry techniques to characterize the bulk and surface properties of probiotic powder particles under different forms and report quantitative results of a highly concentrated multistrain reference product. The solid particle morphology, size/shape distribution, and the powder surface wettability in the compressed disc and porous packed bed forms are successively measured by sessile drop and capillary rise techniques. A complete wettability of the disc surface is observed through equilibrium contact angle measurements for various solvents, whereas the associated capillary rise data exhibit two regimes: a power law regime for the first few moments followed by a second regime, which can be described using Darcy’s law. The use of this modeling approach shows the possibility of assessing the particle-packed bed permeability and porosity. These results open a new route of the structure–activity relationship study on the impact of probiotic solid particles on their functionalities and performance in promoting health benefits, related particularly to the human and animal gut permeability. This statement also strengthens the idea of using the compressed disc technique for easily performing probiotic wettability measurements.

show abstract

Section: Introductionmentioning

confidence: 99%

Bulk and Surface Wettability Characteristics of Probiotic Powders in Their Compressed Disc and Packed-Bed Column Forms

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To date, not much literature is available on the mechanism of lactic acid removal using anion exchange resins and its effect on growth of LAB. Instead, most of the reported studies are focusing on the strategy for solely improving lactic acid production instead of viability of cell biomass that are important in probiotic industry ( Boonmee et al, 2016 ; Nguyen et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Growth Enhancement of Probiotic Pediococcus acidilactici by Extractive Fermentation of Lactic Acid Exploiting Anion-Exchange Resin

et al. 2018

View full text Add to dashboard Cite

Fermentation employing lactic acid bacteria (LAB) often suffers end-product inhibition which reduces the cell growth rate and the production of metabolite. The utility of adsorbent resins for in situ lactic acid removal to enhance the cultivation performance of probiotic, Pediococcus acidilactici was studied. Weak base anion-exchange resin, Amberlite IRA 67 gave the highest maximum uptake capacity of lactic acid based on Langmuir adsorption isotherm (0.996 g lactic acid/g wet resin) compared to the other tested anion-exchange resins (Amberlite IRA 410, Amberlite IRA 400, Duolite A7 and Bowex MSA). The application of Amberlite IRA 67 improved the growth of P. acidilactici about 67 times compared to the control fermentation without resin addition. Nevertheless, the in situ addition of dispersed resin in the culture created shear stress by resins collision and caused direct shear force to the cells. The growth of P. acidilactici in the integrated bioreactor-internal column system containing anion-exchange resin was further improved by 1.4 times over that obtained in the bioreactor containing dispersed resin. The improvement of the P. acidilactici growth indicated that extractive fermentation using solid phase is an effective approach for reducing by-product inhibition and increasing product titer.

show abstract

“…The results showed improved growth rate of adapted bacteria (versus non-adapted bacteria) once exposed to oils, reaching similar or greater viable counts (up to 9 log10 units) than controls grown in the absence of oils. In this sense, probiotic bacteria respond to stress by producing specific substances, such as exopolysaccharides and proteins which may protect cells from further stressors (Nguyen, et al, 2016). Furthermore, this adaptation had a great impact on their probiotic features as detected in vitro, such as tolerance to low pH and bile salts.…”

Section: Discussionmentioning

confidence: 99%

New insights into the molecular effects and probiotic properties of Lactobacillus pentosus pre-adapted to edible oils

García

Montoro

Benomar

et al. 2019

LWT

View full text Add to dashboard Cite

In this study, the survival and growth of seven probiotic Lactobacillus pentosus strains isolated from Aloreña green table olives in the presence of vegetable-based edible oils (i.e., sunflower, olive, linseed, soy, corn, almond and argan) and mint essential oil were determined for the first time. Slight decreases in bacterial viability were observed depending on the strain and oil expsoure, mainly mint essential oil. However, pre-adapting the strains to the corresponding oils significantly increased their cell viabilities. As such, this study examined whether pre-adapting probiotic L. pentosus strains with oils will constitute a new strategy to increase stress resistance, e.g., acids (pH 1.5) or bile (up to 3.6%) in food production and/or during digestion, and improve functional probiotic properties. Improvements in stress resistance were noticed in some pre-adapted strains with oils, such as under acidic and bile conditions; further, pre-adaptations with olive, argan, sunflower and linseed oils induced gene expression (e.g., fus, rpsL, pgm, groEL, enol and prep) for moonlighting proteins involved in several stress responses and other functions. As such, pre-adaptation with vegetable edible oils may represent a novel approach for manufacturing probiotic products by improving the stability of bacteria during industrial processes that would otherwise reduce the viability and functionality of the strains.

show abstract

Biochemical Engineering Approaches for Increasing Viability and Functionality of Probiotic Bacteria

Cited by 46 publications

References 131 publications

Bulk and Surface Wettability Characteristics of Probiotic Powders in Their Compressed Disc and Packed-Bed Column Forms

Bulk and Surface Wettability Characteristics of Probiotic Powders in Their Compressed Disc and Packed-Bed Column Forms

Growth Enhancement of Probiotic Pediococcus acidilactici by Extractive Fermentation of Lactic Acid Exploiting Anion-Exchange Resin

New insights into the molecular effects and probiotic properties of Lactobacillus pentosus pre-adapted to edible oils

Contact Info

Product

Resources

About