Biochemical Heterogeneity Within the Maturing Rabbit Medial Collateral Ligament

Frank, Cyril B.; McDonald, D.; Lieber, Richard L.; Sabiston, P.

doi:10.1097/00003086-198811000-00038

Cited by 39 publications

(15 citation statements)

References 13 publications

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“…A third explanation may be variations in the surface strain along the length and width of ligaments under tension (Butler et al, 1990;Lam et al, 1995). Strain in the midsubstance of a ligament has been measured at approximately 44% of the strain in the total bone-ligament-bone complex, perhaps due to variations in biochemical composition along the ligament length (Frank et al, 1988) or relative sliding of collagen fibrils (Wood et al, 1998). Increases in ACL AM DBI after ACL/MCL transection were expected in light of our previous finding of increased anterior tibial translation (Tapper et al, 2008).…”

Section: Tablementioning

confidence: 99%

ACL/MCL transection affects knee ligament insertion distance of healing and intact ligaments during gait in the Ovine model

Tapper

Funakoshi

Hariu

et al. 2009

Journal of Biomechanics

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Section: Tablementioning

confidence: 99%

ACL/MCL transection affects knee ligament insertion distance of healing and intact ligaments during gait in the Ovine model

Tapper

Funakoshi

Hariu

et al. 2009

Journal of Biomechanics

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“…"Contamination" of scar tissue with adjacent uninjured tissue in healing ligaments thus was minimized. Furthermore, this area was compared only with the analogous area from control animals, since these ligaments previously have been shown to be biochemically heterogeneous along their length (15). Each sample was lyophilized and weighed before being hydrolysed in 2.5 ml of 6 N HCI for 16 hours at 100°C.…”

Section: R O S S L I N K S In L I G a M E N T H E A L I N G 159mentioning

confidence: 99%

Rabbit medial collateral ligament scar weakness is associated with decreased collagen pyridinoline crosslink density

Frank

McDonald

Wilson

et al. 1995

Journal Orthopaedic Research

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119

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This study was carried out to quantify the potential associations between material strength and both collagen concentration and pyridinoline collagen crosslink density in the healing medial collateral ligament of the rabbit and to compare these parameters with those of normal ligaments. The right hindlimbs of 24 skeletally mature (12-month-old) New Zealand White rabbits were subjected to a standardized 4 mm midsubstance "gap" injury to the medial collateral ligament. The animals were killed in groups of six at postoperative intervals of 3, 6, 14, or 40 weeks, and the femur-medial collateral ligament-tibia complexes were mechanically tested in tension to failure. Subsequent to mechanical testing, the failure sites of the ligaments were assessed for concentrations of hydroxyproline and hydroxylysyl pyridinoline. Nine additional rabbits served as age-matched normal controls. In healing ligaments, normal collagen concentrations were reached in less than 14 weeks, but the hydroxylysyl pyridinoline crosslink densities remained low and were only 45% of the control values after 40 weeks of healing. Similarly, mechanical values remained much less than the controls. Linear regression analysis of data on scar tissue alone showed a moderately strong positive correlation between hydroxyproline concentration and material strength (r2 = 0.51, p = 0.0001) but no correlation between crosslink density and strength of scar tissue. A similar pattern of correlation was obtained between the elastic modulus of the scar tissue and the biochemical variables, but the r2 values were lower.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Frank et al report that the biochemical composition is not uniform within the MCL in rabbits of similar ages [31]. However, there is no previous report on the normative collagen fibril profiles across different regions of a ligament.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Collagen Fibril Distribution in the Medial Collateral Ligament in a Rat Knee Model

Fung

Leung

et al. 2003

Connective Tissue Research

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This study compared the collagen fibril diameter distribution among six anatomical sites of the rat medial collateral ligament (MCL). Ultrathin MCL sections from 4 male Sprague-Dawley rats were examined electron microscopically. With an automated quantitation method, 41,638 fibrils were measured and compared among the periphery and core regions of the femoral, middle, and tibial portions of the MCL. Results demonstrated significant difference (p < .0033) in mean fibril diameter distribution among the six sites. The mass-averaged diameters of the core and peripheral fibrils were between 175.53 to 190.82 nm and 88.47 to 109.18 nm, respectively, with the peripheral fibrils more homogeneous in size. The fibrils occupied 36.7% to 57.1% of the cross-sectional area of the ligament. About 50% of the fibrils had an oblique factor of 0.8-1.0, implying that most fibrils were aligned longitudinally. This study has provided a detailed profile of the collagen fibril distributions in rat MCL.

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Biochemical Heterogeneity Within the Maturing Rabbit Medial Collateral Ligament

Cited by 39 publications

References 13 publications

ACL/MCL transection affects knee ligament insertion distance of healing and intact ligaments during gait in the Ovine model

ACL/MCL transection affects knee ligament insertion distance of healing and intact ligaments during gait in the Ovine model

Rabbit medial collateral ligament scar weakness is associated with decreased collagen pyridinoline crosslink density

Investigation of the Collagen Fibril Distribution in the Medial Collateral Ligament in a Rat Knee Model

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