Biochemical mechanisms of resistance TO p-nitrochlorobenzene of karst caves microorganisms

Suslova, Olga; Rokitko, P. V.; Bondar, K.; Golubenko, O. O.; Tashyrev, Oleksandr

doi:10.15407/ubj87.04.032

Cited by 2 publications

(3 citation statements)

References 4 publications

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“…1chloro-3-nitrobenzene was extracted from culture liquid with hexane in a volume ratio of "culture liquidhexane" 1:5. Concentration of CNB and the product of its reduction, chloroaniline (CA), in hexane was measured by absorption in the ultraviolet spectrum (l max CNB = 260 nm, l max CA = 245 nm) [5]. Сoncentration of organic compounds by total carbon was determined by the permanganate method [6].…”

Section: Methodsmentioning

confidence: 99%

“…As evidence, we can mention reductive destruction of CNB followed by decomposition of reduced aromatic ring by the strain Arthrobacter oryzae P11 isolated from the clay of the karst cave Mushkarova Yama [5] outstanding feature. Consequently, a priori there are no microorganisms adapted to nitrochloroaromatic compounds in a cave.…”

Section: Experimental Confirmation Of Thermodynamic Prediction Of Micmentioning

confidence: 97%

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Thermodynamic Prediction for Development of Novel Environmental Biotechnologies and Valuable Products From Waste Obtaining

Tashyrev

Govorukha

Suslova

et al. 2018

EEEP

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The aim of the work was to develop effective environmental biotechnologies enabling to stop pollution of environment with a wide range of toxicants and transform them into commercially valuable products. Using the method of thermodynamic prediction an algorithm to create new universal environmental biotechnologies was developed. Distinctive features of biotechnologies include: 1). fast and efficient disposal of a wide range of environmentally hazardous waste (decaying food and agricultural waste, synthetic organic compounds, and metal-containing waste water, etc.); 2). increase the efficiency of biotechnologies due to application of selected soil anaerobic microorganisms; 3). obtaining of a wide range of valuable products: gaseous fuels (hydrogen, methane), solid fuel (unfermented lignin and cellulose residues), concentrate of nonferrous metals (copper, chromium, etc.) and clean water; 4). ability to scale and replicate typical technologies of designated purpose. Obtained engineering and technological parameters of universal biotechnologies indicate the prospects of their large-scale industrial implementation.

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Section: Methodsmentioning

confidence: 99%

Section: Experimental Confirmation Of Thermodynamic Prediction Of Micmentioning

confidence: 97%

Thermodynamic Prediction for Development of Novel Environmental Biotechnologies and Valuable Products From Waste Obtaining

Tashyrev

Govorukha

Suslova

et al. 2018

EEEP

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“…Microorganisms of karst caves were affiliated with Microbacterium testaceum, Rhodococcus erythropolis, Arthrobacter oryzae and Pseudomonas azotoformans species. The studied strains grew in high concentrations of toxic compounds: 1000 mg / L of Cu 2+ and 300 mg / L of 1-сhloro-4-nitrobenzene and in kopio-oligocarbotrophic conditions [13,23,28]. The specified strains were not only resistant to Cu 2+ and 1-сhloro-4nitrobenzene, but also interacted with these toxic compounds.…”

mentioning

confidence: 99%

Autecology and Taxonomy of Bacteria Isolated from Extreme Environments

Tashyrev¹,

Romanovskaya²,

Rokitko³

et al. 2017

Mikrobiol. Z.

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The paper presents the final results of a five-year research (2011-2015) conducted at the Extremophilic Microorganism Biology Department. Extremotolerant microorganisms were isolated from phytocenoses and soils of West Antarctic; subtropical soils (Israel), karst caves and soils of temperate regions (Ukraine, Bulgaria), silt of hypersaline lakes of Crimea (Ukraine) and the Dead Sea (Israel), highland areas of Andes (Ecuador). 10% of them were resistant to toxic metals, 1-сhloro-4-nitrobenzene, UV radiation, high salinity, as well as to low and high temperatures. A method of thermodynamic prognosis of microbial interaction with various toxic compounds was developed, providing a theoretical background for designing new environmental biotechnologies. It enabled to determine the conditions for microbial metabolism regulation that provide for complete degradation of synthetic organic compounds and food wastes as well as for production of energy carriers (H 2 , CH 4 , ethanol, solid fuel). The specified approach resulted an isolation of a number of strains that were resistant to the most toxic metals (Hg 2+ , Cu 2+ , Ni 2+ , Fe 3+ , Fe 2+ , CrO 4 2-) and performed a variety of interactions with metal compounds including mobilization and immobilization, dissolving and sedimentation. Biodiversity of microorganisms in West Antarctica was studied by means of standard microbiological methods as along with phylogenetic and metagenomic analysis. Pyrosequencing of 16S rDNA amplicons of microbial communities in the studied samples revealed representatives of 20 bacterial phyla. Microbiological methods identified cultivable representatives of four phyla (Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes), which included 25 species. The paper presents the results of taxonomic and/or biotechnology studies of specialized groups of extremophiles: ironreducing bacteria, hydrogen-producing bacteria and obligate methane-oxidizing bacteria.

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Biochemical mechanisms of resistance TO p-nitrochlorobenzene of karst caves microorganisms

Cited by 2 publications

References 4 publications

Thermodynamic Prediction for Development of Novel Environmental Biotechnologies and Valuable Products From Waste Obtaining

Thermodynamic Prediction for Development of Novel Environmental Biotechnologies and Valuable Products From Waste Obtaining

Autecology and Taxonomy of Bacteria Isolated from Extreme Environments

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