Biochemical Pregnancy Diagnosis

Cain, A. J.; Christiansen, David L.

doi:10.1002/9781118833971.ch35

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…The blood samples can then be sent to a laboratory that is capable of measuring pregnancy-associated glycoproteins such as pregnancy-specific protein B (PSPB; Sasser et al 1986). The PSPB (BioPRYN™) ELISA is 93% accurate beginning at ~ Day 30 of gestation, but because of its long half-life it does not make a good pregnancy test in early postpartum cows (Cain and Christiansen 2015).…”

Section: Embryo Transfer Embryo Transfer Is a Reproductivementioning

confidence: 99%

Modern Reproductive Technologies to Improve Cattle Production

Youngs

2016

Ceiba

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Abstract. Cattle contribute significantly to the global supply of animal-derived proteins which are an important part of a well-balanced human diet. With the human population increasing by 1 billion people every 13 years, there will be an estimated 9.6 billion people on planet Earth by the year 2050. To attain global food security the amount of available food will need to double between now and then. Enhancing reproductive efficiency is a prerequisite for boosting production of meat and milk from cattle, and, fortunately, an arsenal of modern reproductive technologies is available to assist with that effort. The objective of this manuscript is to provide an overview of reproductive biotechnologies that can bolster the efficient production of meat and milk from cattle. Protocols for synchronization of estrus and synchronization of ovulation facilitate more efficient artificial insemination using conventional or sexsorted semen. In vivo and in vitro production of preimplantation embryos from genetically superior females enable creation of multiple offspring with high production potential. Biochemical and ultrasonographic methods for pregnancy testing identify non-pregnant females that can be re-mated or sold to prevent wastage of valuable feed resources. Somatic cell nuclear transfer is used to create copies of highly productive animals, and genome editing of zygotes provides a novel opportunity to selectively enhance the genetic makeup of cattle for the benefit of animal and human health. Wise use of these reproductive technologies will increase food production from cattle and will help alleviate world hunger.Key words: Embryo transfer, in vitro fertilization, pregnancy testing, sperm sexing, synchronization of estrus. Tecnologías Reproductivas Modernas para Mejorar la Producción PecuariaResumen. El ganado contribuye significativamente al suministro global de proteínas de origen animal que son una parte importante de una dieta humana bien balanceada. Con el incremento de la población a un ritmo de mil millones de personas cada 13 años, habrá aproximadamente 9.6 mil millones de personas en el año 2050. Para alcanzar una seguridad alimentaria global, la cantidad de comida disponible deberá ser el doble entre la actual y la de esta fecha futura. Mejorar la eficiencia reproductiva es un prerrequisito para aumentar la producción de carne y leche, y afortunadamente, un arsenal de tecnologías reproductivas modernas está disponibles para asistir con este esfuerzo. El objetivo de este artículo es proveer un resumen de las biotecnologías reproductivas que pueden mejorar la eficiencia de producción de carne y leche. Con los protocolos para la sincronización del celo y de la ovulación se logra una inseminación artificial más eficiente usando semen convencional o sexado. La producción in vitro e in vivo de embriones a partir de hembras genéticamente superiores ayuda a la creación de crías múltiples con un alto potencial de producción. Los métodos bioquímicos y ultrasonográficos identifican de hembras vacías que pueden volver ...

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Section: Embryo Transfer Embryo Transfer Is a Reproductivementioning

confidence: 99%

Modern Reproductive Technologies to Improve Cattle Production

Youngs

2016

Ceiba

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“…PAGs are produced from the stage of embryo implantation and continue throughout gestation. 6,7 In fact, PAGs are thought to be involved in maternal recognition of pregnancy. 8 Presence of PAGs in maternal circulation allows for an early diagnosis of pregnancy, 9,10 detection of late embryonic or early fetal deaths, identification of twin pregnancies, and monitoring of placental function.…”

Section: Introductionmentioning

confidence: 99%

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

et al. 2019

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Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations were assessed in Holstein heifers 28 days post-insemination, following estrus synchronization with either a double prostaglandin (PG) injection or the ovsynch protocol. Animals between 15 and 20 months of age, with a 3-3.5 BCS, were randomly assigned to three groups: Heifers in the control group (n = 70) were monitored for estrus behaviour for 21 days and inseminated when detected in standing heat; Estrus of heifers in the Ovsynch group (n = 70)) was synchronized following the Ovsynch protocol, and timed artificial insemination was performed 16 h after the completion of treatment; Animals in the PG group (n = 70) received two prostaglandin-F2α injections 11 days apart, were monitored for estrus behaviour for 2-5 days following the last injection, and inseminated when in heat. Serum PAGs and progesterone concentrations were measured in pregnant heifers (control group n = 25; ovsynch group n = 22; PG group n = 21) on day 28 after artificial insemination (AI). Mean serum PAGs concentrations (OD: optical density) were similar between groups (control = 3.37 ± 0.148, Ovsynch = 3.21 ± 0.136, and PG = 3.34 ± 0.143; p > 0.05). However, serum progesterone concentrations were found to be lower in the ovsynch group (4.70 ± 0.17 ng/mL) when compared to the PG (5.34 ± 0.13 ng/mL) or the control (5.37 ± 0.08 ng/mL) groups (p < 0.001). There was no correlation between serum PAGs and progesterone levels at day 28 post-insemination, or between early pregnancy PAGs concentrations and fetal death rates. Further research, including multiple sampling time points throughout gestation, after estrus synchronization protocols is warranted, to determine if there are associated temporal changes in PAGs and progesterone profiles that could impact reproductive parameters in dairy heifers.

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Early Pregnancy Diagnosis in Dairy Cattle

Akköse¹,

Şen

2019

Hayvansal Üretim

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Sütçü sığırlarda erken gebelik tanısı reprodüktif yönetim açısından oldukça önemlidir. Erken gebelik tanısı, gebe olmayan hayvanların zamanında tedavi edilmeleri ve yeniden tohumlanmaları yoluyla buzağılama aralığındaki uzamayı engeller. Suni tohumlamadan sonraki 18 -24 günler arasında östrusa gelme, gebe olmayan hayvanların tespit edilmesindeki en kolay yöntemdir. Rektal palpasyon ve transrektal ultrasonografi erken gebelik tanısı amacıyla en sık kullanılan klinik yöntemlerdir. Progesteron veya gebelikle ilişkili glikoproteinlerin tespiti esasına dayanan gebelik testleri de erken gebelik tanısı amacıyla sıklıkla kullanılan laboratuvar yöntemleridir. Süt sığırlarında erken gebelik teşhisine yönelik yeni bir yaklaşım sağım sırasında, sağım sistemine yerleştirilmiş bir cihaz tarafından sütte bulunan gebeliğe özgü maddelerle gebeliklerin ve embriyo canlılığının izlenmesidir. Bu yazıda sütçü sığırlarda kullanılan erken gebelik tanı yöntemleri ve gebelik tanısı alanındaki gelişmeler hakkında bilgiler derlenmiştir.

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Biochemical Pregnancy Diagnosis

Cited by 4 publications

References 39 publications

Modern Reproductive Technologies to Improve Cattle Production

Modern Reproductive Technologies to Improve Cattle Production

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

Early Pregnancy Diagnosis in Dairy Cattle

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