Early Pregnancy Diagnosis in Dairy Cattle

Akköse, Mehmet; Şen, Çiğdem Çebi

doi:10.29185/hayuretim.458620

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…Pregnancy-associated glycoproteins (PAG) aresynthesized in the mono-and binucleate cells of the ruminant's trophectoderm (Sousa et al, 2006). PAG can be used for pregnancy diagnosis in ruminants by detecting their presence or concentrations in the maternal blood circulation (Akköse and Cebi-Sen, 2019;Chaves et al, 2017;Silva et al, 2007;Singh et al, 2021). de Carolis et al (2020), reported that the cutoff value of PAG concentrations (radioimmuno assay, RIA-706 and RIA-srPool) are ≥ 1.0 ng/mL in order to distinguish between pregnant and nonpregnant Sarda and Lacaune ewes.…”

Section: Introductionmentioning

confidence: 99%

The prediction of lambing rate in Awassi sheep using a rapid visual PAG ELISA test

Akköse

Şen

Polat

2022

J Hellenic Vet Med Soc

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The accurrate diagnosis of pregnancy and prediction of lambing rate are important tools that ensure the proper nutrition of pregnant ewes, optimize the birth weight of offspring and decrease the occurrence of pregnancy toxemia in sheep production. In this study, it was aimed to evaluate the reliability of the rapid visual PAG ELISA test in predicting the lambing rate, when performed in two different stages of pregnancy in Awassi sheep. A total of 210 ewes, belonging to a commercial flock, were assigned to two groups according to their mating date. Group 1 comprised 120 ewes, which were in the period from 28 to 38 days post-mating, and Group 2 comprised 90 ewes, which were in the period from 40 to 59 days post-mating. The rapid visual PAG ELISA test was performed on the serum samples of the ewes, according to the manufacturer’s instructions. The diagnostic test characteristics of the rapid visual PAG ELISA test were assessed for the prediction of lambing rate, using the lambing records as a gold standard. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of the rapid visual PAG ELISA test were 95.79%, 52.38%, 90.10%, 73.33%, and 87.93%, respectively, in G1 and 92.75%, 70.00%, 91.43%, 73.68%, and 87.64%, respectively, in G2. The McNemar analysis showed that there was no difference between the rapid visual PAG ELISA test and lambing records in the two groups. Eighty per cent all of the ewes exposed to rams lambed. While the rapid visual PAG ELISA test predicted that 83.4% of the ewes exposed to rams would lamb, in reality it achieved that 75.6% of them lambed. The differences between the predicted and achieved lambing rates of rapid visual PAG ELISA (7.8%) indicate embriyonic/fetal mortality. The results of this study indicated that the lambing rate can be predicted with a high sensitivity from the 28th day of pregnancy, using the rapid visual PAG ELISA test.

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Section: Introductionmentioning

confidence: 99%

The prediction of lambing rate in Awassi sheep using a rapid visual PAG ELISA test

Akköse

Şen

Polat

2022

J Hellenic Vet Med Soc

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“…6,7 In fact, PAGs are thought to be involved in maternal recognition of pregnancy. 8 Presence of PAGs in maternal circulation allows for an early diagnosis of pregnancy, 9,10 detection of late embryonic or early fetal deaths, identification of twin pregnancies, and monitoring of placental function. 11,12 Also, early detection of non pregnant animals permitsa prompt reintoduction to appropriate reproductive management practices thereby reducing the calving interval.…”

Section: Introductionmentioning

confidence: 99%

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

et al. 2019

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Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations were assessed in Holstein heifers 28 days post-insemination, following estrus synchronization with either a double prostaglandin (PG) injection or the ovsynch protocol. Animals between 15 and 20 months of age, with a 3-3.5 BCS, were randomly assigned to three groups: Heifers in the control group (n = 70) were monitored for estrus behaviour for 21 days and inseminated when detected in standing heat; Estrus of heifers in the Ovsynch group (n = 70)) was synchronized following the Ovsynch protocol, and timed artificial insemination was performed 16 h after the completion of treatment; Animals in the PG group (n = 70) received two prostaglandin-F2α injections 11 days apart, were monitored for estrus behaviour for 2-5 days following the last injection, and inseminated when in heat. Serum PAGs and progesterone concentrations were measured in pregnant heifers (control group n = 25; ovsynch group n = 22; PG group n = 21) on day 28 after artificial insemination (AI). Mean serum PAGs concentrations (OD: optical density) were similar between groups (control = 3.37 ± 0.148, Ovsynch = 3.21 ± 0.136, and PG = 3.34 ± 0.143; p > 0.05). However, serum progesterone concentrations were found to be lower in the ovsynch group (4.70 ± 0.17 ng/mL) when compared to the PG (5.34 ± 0.13 ng/mL) or the control (5.37 ± 0.08 ng/mL) groups (p < 0.001). There was no correlation between serum PAGs and progesterone levels at day 28 post-insemination, or between early pregnancy PAGs concentrations and fetal death rates. Further research, including multiple sampling time points throughout gestation, after estrus synchronization protocols is warranted, to determine if there are associated temporal changes in PAGs and progesterone profiles that could impact reproductive parameters in dairy heifers.

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İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

Akköse¹

2020

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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The aim of this study was to evaluate two different methods (a bovine rapid visual PAG ELISA test and transabdominal ultrasonography) for early diagnosis of pregnancy in Awassi sheep. The ewes used in this study (n=50) were hand-mated in June of 2019 and lambed in October of 2019. Pregnancy diagnosis was performed 34-38 days after mating by rapid visual PAG ELISA test and transabdominal ultrasonography in standing position. The diagnostic test characteristics of both methods were calculated using lambing results as a gold standard. According to the lambing results, it was determined that, of the 50 ewes, 42 (84%) were pregnant and 8 (16%) were not. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of rapid visual PAG ELISA and transabdominal ultrasonography were 97.6%, 62.5%, 93.2%, 83.3%, 92% and 95.2%, 100%, 100%, 80%, 96%, respectively. In McNemar's analysis, no difference was found between the pregnancy diagnosis methods and lambing results. In conclusion, both rapid visual PAG ELISA and transabdominal ultrasonography in standing position proved to be reliable and practical methods for pregnancy diagnosis as of day 34 post-mating in Awassi ewes.

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Early Pregnancy Diagnosis in Dairy Cattle

Cited by 4 publications

References 57 publications

The prediction of lambing rate in Awassi sheep using a rapid visual PAG ELISA test

The prediction of lambing rate in Awassi sheep using a rapid visual PAG ELISA test

Pregnancy-associated glycoproteins (PAGs) and progesterone concentrations in Holstein heifers following two methods of estrus syncronization

İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

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