İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

Akköse, Mehmet

doi:10.18016/ksutarimdoga.vi.668707

Cited by 3 publications

(2 citation statements)

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“…Today, commercial PAG‐ELISA pregnancy diagnosis test kits are available for use in cattle (Akkose et al., 2019 ; Akköse, 2023 ; Szenci, 2021 ) but have not been validated for specific use in sheep yet. However, the high level of similarity between the molecular structure of bovine and ovine PAGs (Xie et al., 1991 ) allows the safe use of bovine PAG‐ELISA tests in sheep (Akköse, 2020 ; Chaves et al., 2020 ; de Miranda e Silva Chaves et al., 2017 ; Roberts et al., 2019 ; Steckeler et al., 2018 ). Precisely, highly accurate pregnancy diagnoses can be performed on day 33 of gestation in ewes using bovine PAG‐ELISA tests (Rovani et al., 2016 ) and as of day 26 or 30 using bovine visual pregnancy tests (Akköse, 2020 ; Xie et al., 1991 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, the high level of similarity between the molecular structure of bovine and ovine PAGs (Xie et al., 1991 ) allows the safe use of bovine PAG‐ELISA tests in sheep (Akköse, 2020 ; Chaves et al., 2020 ; de Miranda e Silva Chaves et al., 2017 ; Roberts et al., 2019 ; Steckeler et al., 2018 ). Precisely, highly accurate pregnancy diagnoses can be performed on day 33 of gestation in ewes using bovine PAG‐ELISA tests (Rovani et al., 2016 ) and as of day 26 or 30 using bovine visual pregnancy tests (Akköse, 2020 ; Xie et al., 1991 ). The present study strongly supports this argument.…”

Section: Discussionmentioning

confidence: 99%

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Serum pregnancy–associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep

Akköse,

Çınar,

Yazlık

et al. 2024

Veterinary Medicine & Sci

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BackgroundReported as being expressed by mono‐ and binucleate placental cells, pregnancy‐associated glycoproteins (PAGs) are released into the blood circulation from the ruminant placenta. Circulating gestational PAGs levels may differ between sheep breeds.ObjectiveThis study was aimed at the close monitoring of the serum PAGs profiles of Karya and Konya Merino sheep during early pregnancy.MethodsFifteen Karya and 15 Konya Merino ewes were synchronized by a 12‐day treatment with progesterone‐impregnated intravaginal sponges. After the sponges were withdrawn, the ewes were administered 400 IU of equine chorionic gonadotropin. The ewes were allowed to mate naturally, and all animals were sampled for blood as of the day of mating (day 0) at weeks 1, 2, 3, 4 and 5. Pregnancy diagnoses were made by transabdominal ultrasonography at week 5. At weeks 6, 7, 8, 9 and 11, blood samples were collected only from the pregnant ewes. The blood samples were centrifuged at 3000 × g, and extracted sera were stored at −20°C until being used for laboratory analyses. Serum PAGs levels were determined with the aid of a commercial PAG‐enzyme‐linked immunosorbent assay test originally developed for pregnancy diagnosis in cattle. Differences in the between the PAGs levels throughout pregnancy and the group effect (Karya and Konya Merino) were determined with a two‐way mixed analysis of variance. Pairwise comparisons were made using a Bonferroni adjustment.ResultsPAGs levels showed a linear increase with the advance of pregnancy in both Karya and Konya Merino sheep. No difference was detected between the breeds for serum PAGs levels. The serum PAGs levels of the pregnant and non‐pregnant ewes differed as of the fourth week.ConclusionThe serum PAGs levels of the Karya and Konya Merino ewes were similar during the first 11 weeks of gestation, and pregnancy diagnosis could be made based on serum PAGs levels as of the 4th week in both breeds.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serum pregnancy–associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep

Akköse,

Çınar,

Yazlık

et al. 2024

Veterinary Medicine & Sci

Self Cite

View full text Add to dashboard Cite

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Evaluation of bovine visual ELISA test for detection of pregnancy-associated glycoproteins in early pregnancy diagnosis in goats

Doğan

Köse

2022

Small Ruminant Research

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Effect of GnRH Administration on Pregnancy-Associated Glycoproteins in Dairy Sheep with Different Reproductive Status

Yotov,

Sinapov

2023

Acta Scientiae Vet.

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Background: Measurement of pregnancy-associated glycoproteins (PAGs) by radioimmunoassay or enzyme-linked immunosorbent assay has been commonly used for early pregnancy diagnosis in ruminants. However, an accurately pregnancy detection depends on test antibody, breed and number of embryos. Only few studies have conducted to detect or predict animals at risk of late embrionic mortality (LEM) and to use hormonal interventions for embryo losses reducing, but this area is still open in sheep. The current study aimed to evaluate the effect of gonadotropin releasing hormone (GnRH) administration on Day 4 after artificial insemination on the pregnancy-associated glycoproteins in dairy sheep with different reproductive status in relation to pregnancy and late embrionic mortality detection.Materials, Methods & Results: Sixty-five East Friesian sheep were divided in 2 groups - I (Control group, n = 35) and II (GnRH group, n = 30) and sublected to estrus synchronization and artificial insemination (AI). Group I was not treated andGroup II received 50 μg GnRH on Day 4 after AI. PAGs in blood serum were measured by Alertys Ruminant Pregnancy test on Days 4, 12, 20, 25 and 35 after AI and ultrasound pregnancy test was conducted on Days 20, 25, 35 and 60. Reproductive status (non-pregnant, pregnant and animals with LEM) was determined by ultrasound and the results between different groups were compared. The PAGs mean values according to reproductive status and Day after AI were analysed. Accuracy, sensitivity and specificity of the PAGs test for pregnancy diagnosis on Day 35 were calculated. On Day 20 after AI the pregnat sheep (83.3%) in group I tended to be higher than those (77.1%) in group II with decrease of 25.7% and 20% for the same groups on Day 25. On day 25 LEM was recorded in 33.3% and 24% in group I and II, respectively. The ultrasound exams on Day 60 confirmed the results from Day 25 after AI. A total value of non-pegnant, pregnant animals and LEM was 20%, 56.9% and 28.8%, respectively. The mean values of PAGs in animals with the same reproductive status in group I and II no differed statistically between Days 4 and 35 after AI. On Day 25 the PAGs values in LEM groups (0.126 ± 0.072 and 0.179 ± 0.029) were higher than those (0.062 ± 0.038 and 0.083 ± 0.023) in the non-pregnant groups, but no significan difference was deternined. On Day 35 after AI the mean values of PAGs (0.414 ± 0.125 and 0.421 ± 0.121) for the pregnant groups were significantly (P < 0.05) higher than those (0.078 ± 0.053 and 0.093 ± 0.034) for the non-pregnant groups. The values of PAGs in LEM groups on Day 25 (0.099 ± 0.062 and 0.113 ± 0.058) were decreased and close to the mean values in non-pregnant sheep. A significant effect of the day after AI on the PAGs values was evidenced in pregnant animals only (control r = 0.97 and GnRH r = 0.98; P < 0.05). The PAGs started to rise rapidly between Days 25 and 35 after AI. On Day 35 the accuracy (98%), the sensitivity (97.3%) and the specificity (100%) of the PAG test for pregnancy diagnosis were similar to the values (100%) for ultrasound method.Discussion: The gonadotropin treatment on Day 4 after AI tended to improve the reproductive performance in the sheep,but no significant effect of GnRH on the PAGs values in the animals with different reproductive status was found. The PAGs profiles in non-pregnant and LEM sheep were close and distinguishing between non-pregnant and LEM ewes on Day 35 was impossible. The PAGs values in pregnant sheep showed significantly (P < 0.05) increase between Days 25 and 35 after AI with higher mean values in pregnant than non-pregnant groups on Day 35 (P < 0.05). Alertys Ruminant Pregnancy test was a reliable for pregnancy detection in sheep on Day 35 after artificial insemination. Keywords: sheep, GnRH, PAGs, reproductive status, pregnancy.

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İvesi Koyunlarında Gebeliğin Erken Teşhisi İçin Sığır Hızlı Görsel PAG ELISA Testi ve Transabdominal Ultrasonografinin Değerlendirilmesi

Cited by 3 publications

References 24 publications

Serum pregnancy–associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep

Serum pregnancy–associated glycoprotein profiles during early gestation in Karya and Konya Merino sheep

Evaluation of bovine visual ELISA test for detection of pregnancy-associated glycoproteins in early pregnancy diagnosis in goats

Effect of GnRH Administration on Pregnancy-Associated Glycoproteins in Dairy Sheep with Different Reproductive Status

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