Biochemical Profile of the Soybean Seed Embryonic Axis and Its Changes during Accelerated Aging

Abstract

Seed deterioration is an important topic in plant science, as the majority of cultivated species use seeds as their means of propagation; however, due to its complexity, the process of seed deterioration has not yet been completely elucidated. Three soybean cultivars (BMX Raio, BMX Zeus, and DM 53i54) exposed to four distinct periods of accelerated aging (0, 3, 6 and 9 days) in a fully randomized experimental design. Initially, vigor and germination tests were performed. The activity of superoxide dismutase, catalase, ascorbate peroxidase enzymes, hydrogen peroxide, malonaldehyde, DNA oxidation, macromolecules and mineral content, and Maillard reactions were quantified in the embryonic axis. Results showed that DNA did not suffer degradation or oxidation. In terms of consumption of reserves, only sugars were consumed, while levels of protein, starch, and triglycerides were maintained. The Maillard reaction did show potential as an indicator of buffer capacity of protein to ROS. Additionally, levels of catalase and ascorbate peroxidase decreased during the aging process. Moreover, nutrient analysis showed that a high magnesium level in the cultivar bestowed greater resilience to deterioration, which can indicate a potential function of magnesium in the cell structure via reflex in seed aging through seed respiration.

Research Article Evaluation of DNA integrity as a potential marker to detect aging in soybean (<i>Glycine</i> <i>max</i>) seeds

“…In general, most samples could not be distinguished from each other and those that were distinguished based on C t s were also be distinguished based on germination test data, which is technically simpler and cheaper than qPCR. Ebone et al (2020) did not observe degradation or oxidation in embryo DNA of artificially aged soybean seeds. On the other hand, significant differentiation was observed among all positive control samples, including the sample obtained by only 2.5 min heating (Table 3).…”

Viability and performance of wheat seedlings after artificial seed aging

“…deterioration conditions (e.g., lots aged for 5, 6 or 7 days), since differences in this parameter were only observed at advanced deterioration stages (Table 3). ROS production is the main cause of deterioration, with the accumulation of these molecules triggering lipid peroxidation and resulting in higher MDA concentration (Ebone et al, 2020). Thus, lipid peroxidation indicated by MDA quantification is widely used to determine the occurrence of oxidative damage (Soares et al, 2019).…”

“…Interaction between these molecules and ROS causes oxidative damage, compromising the physiological quality of seeds and affecting their performance during germination (Ebone et al, 2019). In seeds, lipid peroxidation is one of the most widely reported metabolic changes related to deterioration (Ebone et al, 2020). High lipid peroxidation caused by ROS results in malondialdehyde production due to interaction with cell membranes.…”

Influence of seed moisture content and storage period on germination and biochemical indices: Lallemantia iberica and Lallemantia royleana

“…These morphological changes cause the membranes of cells to lose their selective permeability (Zhang et al 2021). Therefore, cations, anions, and sugar in cells of seed leach into intercellular spaces (Ebone et al 2020). The highest EC observed in L.iberica seeds than that of L.royleana was probably due to the most signi cant destruction in the cell membrane of L. iberica seeds (Paravar et al 2018).…”