Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas

Nord, Christoffer; Eriksson, Maria; Dicker, Andrea; Eriksson, Anna; Grong, Eivind; Ilegems, Erwin; Mårvik, Ronald; Kulseng, Bård; Berggren, Per Olof; Gorzsás, András; Ahlgren, Ulf

doi:10.1038/s41598-017-07015-z

Cited by 10 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Additionally, the background strain of leptin-deficient mice influences the phenotype. C57BLKS background leads to an uncontrolled rise in blood sugar, severe depletion of the insulin-producing beta-cells of the pancreatic islets, and death by 10 months of age [ 157 ]. Mice with the C57BL/6 background have compensatory hyperplasia of the islet B cells, and continued hyperinsulinemia throughout an 18- to 20-month life span.…”

Section: Resultsmentioning

confidence: 99%

Wound Healing Impairment in Type 2 Diabetes Model of Leptin-Deficient Mice—A Mechanistic Systematic Review

Stachura

Khanna

Krysiak

et al. 2022

IJMS

View full text Add to dashboard Cite

Type II diabetes mellitus (T2DM) is one of the most prevalent diseases in the world, associated with diabetic foot ulcers and impaired wound healing. There is an ongoing need for interventions effective in treating these two problems. Pre-clinical studies in this field rely on adequate animal models. However, producing such a model is near-impossible given the complex and multifactorial pathogenesis of T2DM. A leptin-deficient murine model was developed in 1959 and relies on either dysfunctional leptin (ob/ob) or a leptin receptor (db/db). Though monogenic, this model has been used in hundreds of studies, including diabetic wound healing research. In this study, we systematically summarize data from over one hundred studies, which described the mechanisms underlying wound healing impairment in this model. We briefly review the wound healing dynamics, growth factors’ dysregulation, angiogenesis, inflammation, the function of leptin and insulin, the role of advanced glycation end-products, extracellular matrix abnormalities, stem cells’ dysregulation, and the role of non-coding RNAs. Some studies investigated novel chronic diabetes wound models, based on a leptin-deficient murine model, which was also described. We also discussed the interventions studied in vivo, which passed into human clinical trials. It is our hope that this review will help plan future research.

show abstract

Section: Resultsmentioning

confidence: 99%

Wound Healing Impairment in Type 2 Diabetes Model of Leptin-Deficient Mice—A Mechanistic Systematic Review

Stachura

Khanna

Krysiak

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Although beta cell connectivity was increased in the 10month-old ob/ob mice, not all aspects of their islet function were improved. Indeed, it was shown using vibrational microscopy and multivariate analysis, that reporter islets transplanted into the ACE of ob/ob mice had a higher content of collagen (44). This technique allows to register changes in the chemical composition of tissues and in this case reported on an increase in blood vessel fibrosis in ob/ob mouse islets.…”

Section: Functional Plasticity Of Pancreatic Isletsmentioning

confidence: 99%

The Eye as a Transplantation Site to Monitor Pancreatic Islet Cell Plasticity

Ilegems

Berggren

2021

Front. Endocrinol.

Self Cite

View full text Add to dashboard Cite

The endocrine cells confined in the islets of Langerhans are responsible for the maintenance of blood glucose homeostasis. In particular, beta cells produce and secrete insulin, an essential hormone regulating glucose uptake and metabolism. An insufficient amount of beta cells or defects in the molecular mechanisms leading to glucose-induced insulin secretion trigger the development of diabetes, a severe disease with epidemic spreading throughout the world. A comprehensive appreciation of the diverse adaptive procedures regulating beta cell mass and function is thus of paramount importance for the understanding of diabetes pathogenesis and for the development of effective therapeutic strategies. While significant findings were obtained by the use of islets isolated from the pancreas, in vitro studies are inherently limited since they lack the many factors influencing pancreatic islet cell function in vivo and do not allow for longitudinal monitoring of islet cell plasticity in the living organism. In this respect a number of imaging methodologies have been developed over the years for the study of islets in situ in the pancreas, a challenging task due to the relatively small size of the islets and their location, scattered throughout the organ. To increase imaging resolution and allow for longitudinal studies in individual islets, another strategy is based on the transplantation of islets into other sites that are more accessible for imaging. In this review we present the anterior chamber of the eye as a transplantation and imaging site for the study of pancreatic islet cell plasticity, and summarize the major research outcomes facilitated by this technological platform.

show abstract

“…In addition, light has a potentiating effect on islets, potentially affecting insulin secretion and control ( 66 ). Finally, transplantation in the eye may result in the formation of cataracts, and collagen deposition into the eye blood vessels ( 70 , 140 ). Anterior chamber of the eye is promising islet transplantation site, as demonstrated in different animal models including non-human primates but there are hesitations to its clinical applicability especially with the eye being a very sensitive and important organ.…”

Section: Transplantation Sitesmentioning

confidence: 99%

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes

2021

View full text Add to dashboard Cite

Diabetes is a complex disease that affects over 400 million people worldwide. The life-long insulin injections and continuous blood glucose monitoring required in type 1 diabetes (T1D) represent a tremendous clinical and economic burdens that urges the need for a medical solution. Pancreatic islet transplantation holds great promise in the treatment of T1D; however, the difficulty in regulating post-transplantation immune reactions to avoid both allogenic and autoimmune graft rejection represent a bottleneck in the field of islet transplantation. Cell replacement strategies have been performed in hepatic, intramuscular, omentum, and subcutaneous sites, and have been performed in both animal models and human patients. However more optimal transplantation sites and methods of improving islet graft survival are needed to successfully translate these studies to a clinical relevant therapy. In this review, we summarize the current progress in the field as well as methods and sites of islet transplantation, including stem cell-derived functional human islets. We also discuss the contribution of immune cells, vessel formation, extracellular matrix, and nutritional supply on islet graft survival. Developing new transplantation sites with emerging technologies to improve islet graft survival and simplify immune regulation will greatly benefit the future success of islet cell therapy in the treatment of diabetes.

show abstract

Biochemical profiling of diabetes disease progression by multivariate vibrational microspectroscopy of the pancreas

Cited by 10 publications

References 43 publications

Wound Healing Impairment in Type 2 Diabetes Model of Leptin-Deficient Mice—A Mechanistic Systematic Review

Wound Healing Impairment in Type 2 Diabetes Model of Leptin-Deficient Mice—A Mechanistic Systematic Review

The Eye as a Transplantation Site to Monitor Pancreatic Islet Cell Plasticity

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes

Contact Info

Product

Resources

About