Biochemical response in gilthead seabream (Sparus aurata) to in vivo exposure to a mix of selected PAHs

Kopecka-Pilarczyk, Justyna; Correia, Ana D.

doi:10.1016/j.ecoenv.2008.12.003

Cited by 50 publications

(17 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The latter was further reinforced by the significant correlation obtained among NRRT, TNA and LPO with TRS measured in tissues of mussels. The increased levels of MDA, measured in gills of PAH-exposed mussels, are in accordance with previous studies (Frouin et al, 2007;Giannapas et al, 2012;Kopecka-Pilarczyk and Correia, 2009), thus indicating PAH-mediated oxidative effects. However, although MDA could reflect a valuable marker of oxidative stress, its short-half life could lead to incorrect results, concerning prooxidants ability to enhance oxidative damage within cells (Siu and Draper, 1982).…”

Section: Tablesupporting

confidence: 91%

Total thiol redox status as a potent biomarker of PAH-mediated effects on mussels

Grintzalis

Georgiou

Dailianis

2012

Marine Environmental Research

View full text Add to dashboard Cite

Section: Tablesupporting

confidence: 91%

Total thiol redox status as a potent biomarker of PAH-mediated effects on mussels

Grintzalis

Georgiou

Dailianis

2012

Marine Environmental Research

View full text Add to dashboard Cite

“…The lack of a PAH effect on EROD activity could be explained by the antagonist effect of the different PAHs used for the exposure. In fact, BaP is considered as an inductor of the EROD activity, while an inhibitory effect on this activity was already demonstrated in fish for Fluo and Pyr (Willett et al, 2001;Kopecka-Pilardczyk and Correia, 2009). Comet data were analyzed by a main effect ANOVA (Statistica 8.0 Soft).…”

Section: Introductionmentioning

confidence: 99%

Relationship between PAH biotransformation as measured by biliary metabolites and EROD activity, and genotoxicity in juveniles of sole (Solea solea)

Wessel

Santos

Ménard

et al. 2010

Marine Environmental Research

View full text Add to dashboard Cite

Polycylic aromatic hydrocarbons (PAHs) are ubiquitous contaminants in the marine environment. Their toxicity is mainly linked to the ability of marine species to biotransform them into reactive metabolites. PAHs are thus often detected at trace levels in animal tissues. For biomonitoring purposes, this findings have two main consequences, (i) the determination of the PAH tissue concentration is not suitable for the evaluation of individual exposure to PAHs (ii) it can explain sometimes the lack of correlations obtained with relevant markers of toxicity such as genotoxicity biomarkers. The aim of the present study was to better investigate the link between PAH exposure and genotoxicity in marine flatfish. During a laboratory experiment, juvenile soles were exposed for four weeks to a mixture of three PAHs, namely benzo[a]pyrene, fluoranthene and pyrene, followed by one week of depuration. Fish were exposed via the trophic route to a daily PAH concentration of 120 μg/g food. Fish were sampled at different time points. The bioavailability and the biotransformation of PAHs were assessed by the measurement of biliary metabolites using a sensitive UPLC MS/MS method. The 7-ethoxyresorufine-O-deethylase was also measured in liver subcellular fractions as a biomarker of phase I biotransformation activities. Genotoxicity was assessed in parallel by the measurement of DNA strand breaks in fish erythrocytes by the alkaline comet assay. During this study, the high amount of PAH metabolites produced in sole demonstrated the bioavailability of PAHs and their biotransformation by fish enzymes. A positive correlation was observed between the level of hydroxylated PAH metabolites and genotoxicity as measured by the alkaline comet assay.

show abstract

“…Several studies have evaluated the effects of ABC transporters in fish exposed to environmental pollutants (Christine Paetzold et al, 2009;Diaz de Cerio et al, 2012). Phase I (e.g., Cyp450) and II (e.g., Gst) detoxification enzymes catalyze the reactions of xenobiotic metabolism, and the induction of phase I and II enzymes by environmental pollutants (e.g., PAHs) has been widely studied (Kopecka-Pilarczyk and Correia, 2009;Lu et al, 2009;Yeager et al, 2009). Previous studies indicated that ABC transporters could be regulated in a coordinated manner with phase I and II enzymes (Bard, 2000;Leslie et al, 2005;Xu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)

Yuan

Zha

et al. 2014

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

a b s t r a c t ATP-binding cassette (ABC) transporters together with phase I and II detoxification enzymes have been considered as included in a cellular detoxification system. Previous studies have highlighted the involvement of aryl hydrocarbon receptor (AHR) and Cyp1a in PAH-induced embryo toxicity. However, the response of other xenobiotic enzymes/transporters in PAH-mediated embryo toxicity is not fully characterized. In the present study, rare minnow embryos were exposed to 10 and 100 mg/L BaP within 4 h post-fertilization (hpf) up to 168 hpf. RNA was extracted at 24, 48, 96, and 168 hpf. The basal and BaP-induced expression of phase I enzyme genes (cyp1a, 1b1, and 1c1), phase II enzyme gene (gstm and ugt1a), and ABC transporter genes (abcb1, abcc1, abcc2, and abcg2) mRNA was determined using realtime PCR. Severe developmental defects (e.g., spinal deformities, pericardial and yolk-sac edema) were observed in the BaP treated groups. The basal expression showed that gstm was most strongly expressed, followed by abcb1, ugt1a, and abcc2, whereas cyp1a, 1b1, 1c1, and abcg2 showed weak expression. BaP significantly induced the mRNA expression of three CYP1s (cyp1a, 1b1, and 1c1) (p o0.05) and the ABC transporters (abcc1, abcc2, and abcg2) in a dose-dependent manner. However, the mRNA expression of Phase II enzymes (gstm, ugt1a) for the BaP treatments showed no significant difference with that of the controls. Furthermore, distinct induced patterns of these genes were observed during different exposure periods. Simultaneous up-regulation of the cyp and ABC transporter gene transcripts suggests that a possible involvement and cooperation in the detoxification process could provide protection against the BaP toxicity of rare minnows at the early life stage.

show abstract

Biochemical response in gilthead seabream (Sparus aurata) to in vivo exposure to a mix of selected PAHs

Cited by 50 publications

References 30 publications

Total thiol redox status as a potent biomarker of PAH-mediated effects on mussels

Total thiol redox status as a potent biomarker of PAH-mediated effects on mussels

Relationship between PAH biotransformation as measured by biliary metabolites and EROD activity, and genotoxicity in juveniles of sole (Solea solea)

Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)

Contact Info

Product

Resources

About