“…Abbreviations: DO, dissolved oxygen concentration; OAA, oxaloacetate; PEP, phosphoenolpyruvate; PMLA, β-poly(l-malic acid); TCA, tricarboxylic acid is a polymorphic fungus, well known as the producer of the commercial polysaccharide, pullulan, and other valuable bioproducts [5]. Recent work by Manitchotpisit et al [5] reported that most strains of A. pullulans in genetically diverse phylogenetic clades could produce PMLA.Ĉernáková et al [6] found that A. pullulans could utilize a wide variety of carbon sources, such as d-xylose, lactose, sucrose, maltose, cellobiose, inulin, soluble starch, nonglycosidic substrates, etc. So far, there have been very limited publications available regarding the effects of carbon sources on PMLA production in flask [7][8][9], and the mechanism of carbon source on PMLA production was not explained.…”