Biochemical studies on some aspects of protein metabolism in vitamin-D deficient rats

Al-Gauhari, A.A.M.I.; Al-Nagdy, S.A.; El-Sabbagh, Mahmoud; Eisa, E. A.

doi:10.1016/0300-9629(74)90459-9

Cited by 3 publications

(2 citation statements)

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“…The influence of vitamin D on intestinal transport of calcium and phosphorus and the influence of the vitamin on the maintenance of normal skeletal growth have been well established (1)(2)(3)(4)(5)(6)(8)(9)(10). Although muscle wasting and weakness are prominent features of nutritional rickets (16)(17)(18)(19), a direct action of the vitamin on muscle metabolism and growth has heretofore not been appreciated.…”

Section: Discussionmentioning

confidence: 99%

“…Stimulation of growth of the vitamin D-deficient patient or experimental animal has long been a recognized response to vitamin D replacement therapy (1)(2)(3). This increase in musculoskeletal growth has been attributed to the specific action of vitamin D on calcium homeostasis and specifically, the increase in intestinal absorption of calcium and secondarily an increase in bone mineralization and growth (4,5).…”

Section: Introductionmentioning

confidence: 99%

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25-hydroxycholecalciferol stimulation of muscle metabolism.

Birge¹,

Haddad²

1975

J. Clin. Invest.

241

111

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A B S T R A C T Intact diaphragms from vitamin D-deficient rats were incubated in vitro with ['H]leucine. Oral administration of 10 ug (400 U) of cholecalciferol 7 h before incubation increased leucine incorporation into diaphragm muscle protein by 136% (P <0.001) of the preparation from untreated animals. Nephrectomy did not obliterate this response. ATP content of the diaphragm muscle was also enhanced 7 h after administration of the vitamin. At 4 h after administration of cholecalciferol, serum phosphorus concentration was reduced by 0.7 mg/100 ml (P <0.025) and the rate of inorganic 'PO. accumulation by diaphragm muscle was increased by 18% (P < 0.025) over the untreated animals. Increasing serum phosphate concentration of the vitamin D-deficient animals by dietary supplementation with phosphate for 3 days failed to significantly enhance leucine incorporation into protein. However, supplementation of the rachitogenic, vitamin D-deficient diet with phosphorus for 3 wk stimulated the growth of the animal and muscle ATP levels. This increase in growth and muscle ATP content attributed to the addition of phosphorus to the diet was less than the increase in growth and muscle ATP levels achieved by the addition of both phosphorus and vitamin D to the diet.To eliminate systemic effects of the vitamin, the epitrochlear muscle of the rat foreleg of vitamin D-depleted rats was maintained in tissue culture. Addition of 20 ng/ml of Dr. Birge is a recipient of an American Heart Association Investigatorship. Dr. Haddad is a recipient of a National Institutes of Health Career Development Award.Received for publication 24 February 1975 and in revised form 9 July 1975. pg/ml were without effect. Analysis of muscle cytosol in sucrose density gradients revealed a protein fraction which specifically bound 25-OHD3 and which demonstrated a lesser affinity for 1,25-(OH)2D3. These studies suggest that 25-OHD3 may influence directly the intracellular accumulation of phosphate by muscle and thereby play an important role in the maintenance of muscle metabolism and function.

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