The present study has been conducted to evaluate the characterization of the oil extracted from local cultivated sesame seeds that produced under Syrian conditions, and to explore the effect of gamma irradiation (3, 6 and 9 kGy) and storage period (6 and 12 months) on some quality characteristics of sesame seed oil. The physico-chemical properties of oil extracted from non-irradiated samples of sesame seeds were: acid value (AV) (2.79 mg KOH g -1 oil), peroxide value (PV) (1.69 meq O 2 kg -1 oil), Thiobarbituric acid (TBA) (0.022 mg MDA kg -1 oil), iodine value (IV) (98.36 g I 2 100 g -1 oil), saponification value (SV) (186.27 mg KOH g -1 oil), refractive index (RI) (1.471) and color values (L* = 90.14, a* = 50.76, b* = 30.47 and ∆E = 68.23). The results demonstrated that the AV, PV, TBA, IV, SN, RI and color values were significantly (p<0.05) altered upon irradiation and storage. Gamma irradiation and storage time increased AV, PV, TBA, IV, SN, and RI. However, all tested markers of oil that extracted from irradiated and un-irradiated seeds field within the limited value recommended by Codex Alimentarius Commission.