Biochemistry of Citric Acid Production from Rapeseed Oil by <i>Yarrowia lipolytica</i> Yeast

Kamzolova, Svetlana V.; Lunina, Julia N.; Morgunov, Igor G.

doi:10.1007/s11746-011-1954-1

Cited by 63 publications

(57 citation statements)

References 37 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mutant strain Y. lipolytica NG40/UV5 was obtained as described previously [9,24]. Chemicals, the manufacturer of the carbon sources, and their characteristics were presented in our published articles [7,9,15,17].…”

Section: Microorganisms and Chemicalsmentioning

confidence: 99%

“…Chemicals, the manufacturer of the carbon sources, and their characteristics were presented in our published articles [7,9,15,17].…”

Section: Microorganisms and Chemicalsmentioning

confidence: 99%

“…Earlier, it was found that the mass yield of CA production (Y CA ), expressed in g of CA per g of carbon source, and the fermenter productivity, expressed in g (L·h) −1 were influenced by the medium dilution due to the addition of NaOH solution for maintaining a constant pH value [9,17]. In this regard, the total amount of CA in the culture broth at the end of the fermentation was used to calculate Y CA and fermenter productivity.…”

Section: Calculationsmentioning

confidence: 99%

“…In this regard, the total amount of CA in the culture broth at the end of the fermentation was used to calculate Y CA and fermenter productivity. Formulas for calculation of Y CA value and fermenter productivity were described earlier [9,17].…”

Section: Calculationsmentioning

confidence: 99%

“…To carry out fermentation processes, in addition to very expensive food raw materials, such as glucose [4][5][6][7] and plant oils [8][9][10], much cheaper substrates which are waste products of various industries, such as glycerol-containing waste of the biodiesel industry [9][10][11][12][13][14][15][16][17], glucose-containing wood hydrolysates [18,19], olive mill waste-water [20], and inulin [21] are used. Ethanol, a water-soluble individual compound which ensures the formation of a pure product and facilitates the isolation process, is also of great importance [22,23].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

2018

View full text Add to dashboard Cite

Abstract:The world market of citric acid (CA) is one of the largest and fastest growing markets in the biotechnological industry. Microbiological processes for CA production have usually used the mycelial fungi Aspergillus niger as a producer and molasses as a carbon source. In this paper, we propose methods for CA production from renewable carbon substrates (rapeseed oil, glucose, glycerol, ethanol, glycerol-containing waste of biodiesel industry and glucose-containing aspen waste) by the mutant strain Yarrowia lipolytica NG40/UV5. It was revealed that Y. lipolytica grew and synthesized CA using all tested raw materials. The obtained results are sufficient for industrial use of most of the raw materials studied for CA production. Using rapeseed oil, ethanol and raw glycerol (which is an important feedstock of biodiesel production), a high CA production (100-140 g L −1 ) was achieved.

show abstract

Section: Microorganisms and Chemicalsmentioning

confidence: 99%

“…Chemicals, the manufacturer of the carbon sources, and their characteristics were presented in our published articles [7,9,15,17].…”

Section: Microorganisms and Chemicalsmentioning

confidence: 99%

Section: Calculationsmentioning

confidence: 99%

Section: Calculationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

2018

View full text Add to dashboard Cite

show abstract

Succinic acid production from n‐alkanes

Kamzolova

Vinokurova

Yusupova

et al. 2012

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

A novel process of production of succinic acid (SA) has been developed, which includes the synthesis of alpha‐ketoglutaric acid by a thiamine‐auxotrophic yeast strain Yarrowia lipolytica VKM Y‐2412 from n‐alkanes and subsequent oxidation of the acid by hydrogen peroxide to SA. The concentration of SA in the culture broth and its yield were found to be 38.8 g/L and 82.45% of n‐alkane consumed, respectively. The isolation procedure involved the extraction of the residual alkanes with the mixture of ethyl acetate and hexane, the decomposition of H2O2 in the filtrate followed by filtrate bleaching and acidification with a mineral acid; the evaporation of filtrate and the ethanol extraction of SA from lyophilized residue. The purity of the SA isolated from the culture liquid filtrate reached 99.5%.

show abstract

Early‐stage sustainability assessment of biotechnological processes: A case study of citric acid production

Becker

Kohlheb

Hunger

et al. 2019

Engineering in Life Sciences

View full text Add to dashboard Cite

Sustainability assessment using a life‐cycle approach is indispensable to contemporary bioprocess development. This assessment is particularly important for early‐stage bioprocess development. As early‐stage investigations of bioprocesses involve the evaluation of their ecological and socioeconomic effects, they can be adjusted more effectively and improved towards sustainability, thereby reducing environmental risk and production costs. Early‐stage sustainability assessment is an important precautionary practice and, despite limited data, a unique opportunity to determine the primary impacts of bioprocess development. To this end, a simple and robust method was applied based on the standardized life‐cycle sustainability assessment methodology and commercially available datasets. In our study, we elaborated on the yeast‐based citric acid production process with Yarrowia lipolytica assessing 11 different substrates in different process modes. The focus of our analysis comprised both cultivation and down‐stream processing. According to our results, the repeated batch raw glycerol based bioprocess alternative showed the best environmental performance. The second‐ and third‐best options were also glycerol‐based. The least sustainable processes were those using molasses, chemically produced ethanol, and soy bean oil. The aggregated results of environmental, economic, and social impacts display waste frying oil as the best‐ranked alternative. The bioprocess with sunflower oil in the batch mode ranked second. The least favorable alternatives were the chemically produced ethanol‐, soy oil‐, refined glycerol‐, and molasses‐based citric acid production processes. The scenario analysis demonstrated that the environmental impact of nutrients and wastewater treatment is negligible, but energy demand of cultivation and down‐stream processing dominated the production process. However, without energy demand the omission of neutralizers almost halves the total impact, and neglecting pasteurization also considerably decreases the environmental impact.

show abstract

Biochemistry of Citric Acid Production from Rapeseed Oil by Yarrowia lipolytica Yeast

Cited by 63 publications

References 37 publications

Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

Succinic acid production from n‐alkanes

Early‐stage sustainability assessment of biotechnological processes: A case study of citric acid production

Contact Info

Product

Resources

About