Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

Morgunov, Igor G.; Kamzolova, Svetlana V.; Lunina, Julia N.

doi:10.3390/fermentation4020036

Cited by 56 publications

(40 citation statements)

References 28 publications

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“…Citric acid is a compound that finds numerous applications as an acidulant, flavoring agent and antioxidant within beverages and confectionery, infant formulas, the chemical and pharmaceutical industries [26,35]. Citric acid is recognized as safe, has a pleasant acid taste, and high water solubility as well as chelating and buffering properties [36].…”

Section: Introductionmentioning

confidence: 99%

Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain

et al. 2019

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Olive mill wastewaters (OMW) are the major effluent deriving from olive oil production and are considered as one of the most challenging agro-industrial wastes to treat. Crude glycerol is the main by-product of alcoholic beverage and oleochemical production activities including biodiesel production. The tremendous quantities of glycerol produced worldwide represent a serious environmental challenge. The aim of this study was to assess the ability of Yarrowia lipolytica strain ACA-DC 5029 to grow on nitrogen-limited submerged shake-flask cultures, in crude glycerol and OMW blends as well as in media with high initial glycerol concentration and produce biomass, cellular lipids, citric acid and polyols. The rationale of using such blends was the dilution of concentrated glycerol by OMW to (partially or fully) replace process tap water with a wastewater stream. The strain presented satisfactory growth in blends; citric acid production was not affected by OMW addition (Citmax~37.0 g/L, YCit/Glol~0.55 g/g) and microbial oil accumulation raised proportionally to OMW addition (Lmax~2.0 g/L, YL/X~20% w/w). Partial removal of color (~30%) and phenolic compounds (~10% w/w) of the blended media occurred. In media with high glycerol concentration, a shift towards erythritol production was noted (Erymax~66.0 g/L, YEry/Glol~0.39 g/g) simultaneously with high amounts of produced citric acid (Citmax~79.0 g/L, YCit/Glol~0.46 g/g). Fatty acid analysis of microbial lipids demonstrated that OMW addition in blended media and in excess carbon media with high glycerol concentration favored oleic acid production.

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Section: Introductionmentioning

confidence: 99%

Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain

et al. 2019

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“…The elevated yields for both biomass and citric acid when crude glycerol was used (Table 2) could have been attributed to the presence of fatty acids in crude glycerol fraction (Figure 4), which stimulated the enzymatic activity of the lipophilic yeast cells, generating a higher production of metabolites or biomass [65,66]. As Morgunov et al (2013) [65] implied, the utilization of waste glycerol for the cultivation of Y. lipolytica strain NG40/UV7 increased the citric acid formation yield with 40.63% compared with the results obtained for pure glycerol [65].…”

Section: Resultsmentioning

confidence: 99%

Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of Candida zeylanoides Yeast Strain ATCC 20367

et al. 2019

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Used kitchen oil represents a feasible and renewable biomass to produce green biofuels such as biodiesel. Biodiesel production generates large amounts of by-products such as the crude glycerol fraction, which can be further used biotechnologically as a valuable nutrient for many microorganisms. In this study, we transesterified used kitchen oil with methanol and sodium hydroxide in order to obtain biodiesel and crude glycerol fractions. The crude glycerol fraction consisting of 30% glycerol was integrated into a bioreactor cultivation process as a nutrient source for the growth of Candida zeylanoides ATCC 20367. Cell viability and biomass production were similar to those obtained with batch cultivations on pure glycerol or glucose as the main nutrient substrates. However, the biosynthesis of organic acids (e.g., citric and succinic) was significantly different compared to pure glycerol and glucose used as main carbon sources.

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“…Previous study has demonstrated that during the YH transition in Y. lipolytica response to adverse conditions, the YlMHY1 gene expression dramatically increased [142]. [28,141,165] Deletion of YlMHY1 gene does not affect cell viability but results in complete inability to undergo mycelial growth, suggesting that YlMHY1 gene expression is the necessary cause for filamentation and acts as positive effectors in dimorphic transition [142]. Currently, it was demonstrated that disruption of YlMHY1 in Y. lipolytica increased intracellular oil accumulation due to the upregulation of citrate synthases, which further improved the citrate production and the followed direction of carbon flux towards the lipid biosynthesis rather than amino acid metabolism [150].…”

Section: Controlling Cell Shape By Gene Regulation Of Yeast-to-hypha mentioning

confidence: 96%

“…Furthermore, optimization of the carbon sources and cultivation conditions are correlated to the level of citric acid production. Current report revealed that high citric acid production (100–140 g/L) was obtained by feeding rapeseed oil, ethanol and raw glycerol in the fermentation with the Y. lipolytica NG40/UV5 strain .…”

Section: Y Lipolytica As the Biomanufacturing Platformmentioning

confidence: 99%

Cellular and metabolic engineering of oleaginous yeastYarrowia lipolyticafor bioconversion of hydrophobic substrates into high‐value products

Soong

Liu

Yoon

et al. 2019

Engineering in Life Sciences

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The non‐conventional oleaginous yeast Yarrowia lipolytica is able to utilize both hydrophilic and hydrophobic carbon sources as substrates and convert them into value‐added bioproducts such as organic acids, extracellular proteins, wax esters, long‐chain diacids, fatty acid ethyl esters, carotenoids and omega‐3 fatty acids. Metabolic pathway analysis and previous research results show that hydrophobic substrates are potentially more preferred by Y. lipolytica than hydrophilic substrates to make high‐value products at higher productivity, titer, rate, and yield. Hence, Y. lipolytica is becoming an efficient and promising biomanufacturing platform due to its capabilities in biosynthesis of extracellular lipases and directly converting the extracellular triacylglycerol oils and fats into high‐value products. It is believed that the cell size and morphology of the Y. lipolytica is related to the cell growth, nutrient uptake, and product formation. Dimorphic Y. lipolytica demonstrates the yeast‐to‐hypha transition in response to the extracellular environments and genetic background. Yeast‐to‐hyphal transition regulating genes, such as YlBEM1, YlMHY1 and YlZNC1 and so forth, have been identified to involve as major transcriptional factors that control morphology transition in Y. lipolytica. The connection of the cell polarization including cell cycle and the dimorphic transition with the cell size and morphology in Y. lipolytica adapting to new growth are reviewed and discussed. This review also summarizes the general and advanced genetic tools that are used to build a Y. lipolytica biomanufacturing platform.

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Citric Acid Production by Yarrowia lipolytica Yeast on Different Renewable Raw Materials

Cited by 56 publications

References 28 publications

Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain

Production of Added-Value Chemical Compounds through Bioconversions of Olive-Mill Wastewaters Blended with Crude Glycerol by a Yarrowia lipolytica Strain

Biodiesel-Derived Glycerol Obtained from Renewable Biomass—A Suitable Substrate for the Growth of Candida zeylanoides Yeast Strain ATCC 20367

Cellular and metabolic engineering of oleaginous yeastYarrowia lipolyticafor bioconversion of hydrophobic substrates into high‐value products

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