Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel

Farooq, Amina; Butt, Nauman Zafar; Hassan, Umer

doi:10.1038/s41598-021-91109-2

Cited by 8 publications

(5 citation statements)

References 64 publications

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“…Because the characteristics of cells in different frequency ranges are different, studies have shown that the excitation frequency is usually more sensitive to cell size when the excitation frequency is around 0.5 MHz. ,− To ensure that the output signal has a sufficiently high SNR, the frequency of the excitation signal in this experiment was set to 500 kHz. In addition, the typical sample concentration in the experiment was around 10 5 particles/mL, a syringe pump (LSP01-1B, Baoding Longer Precision Pump Co., Ltd.) was set at 1.2 μL/min for fluidic control, and the speed of the cells in the microchannel was about 0.01–0.04 m/s.…”

Section: Resultsmentioning

confidence: 99%

Floating-Electrode-Enabled Impedance Cytometry for Single-Cell 3D Localization

Feng

Zhu

et al. 2023

Anal. Chem.

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As a label-free, low-cost, and noninvasive tool, impedance measurement has been widely used in single-cell characterization analysis. However, due to the tiny volume of cells, the uncertainty of the spatial position in the microchannel will bring measurement errors in single-cell electrical parameters. To overcome the issue, we designed a novel microdevice configured with a coplanar differential electrode structure to accurately resolve the spatial position of single cells without constraining techniques such as additional sheath fluids or narrow microchannels. The device precisely localizes single cells by measuring the induced current generated by the combined action of the floating electrode and the differential electrodes when single cells flow through the electrode-sensing area. The device was experimentally validated by measuring 6 μm yeast cells and 10 μm particles, achieving spatial localization with a resolution down to 2.1 μm (about 5.3% of the channel width) in lateral direction and 1.2 μm (about 5.9% of the channel height) in the vertical direction at a flow rate of 1.2 μL/min. In addition, by comparing measurement of yeast cells and particles, it was demonstrated that the device not only localizes the single cells or particles but also simultaneously characterizes their status properties such as velocity and size. The device offers a competitive electrode configuration in impedance cytometry with the advantages of simple structure, low cost, and high throughput, promising cell localization and thus electrical characterization.

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Section: Resultsmentioning

confidence: 99%

Floating-Electrode-Enabled Impedance Cytometry for Single-Cell 3D Localization

Feng

Zhu

et al. 2023

Anal. Chem.

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“…Platinum electrodes were assigned a relative permittivity of 7 (ε r ) and electrical conductivity of 8.9 × 10 6 S/m. Similar material properties were used in our earlier simulation studies as well. , …”

Section: Methodsmentioning

confidence: 99%

“…Similar material properties were used in our earlier simulation studies as well. 21,22 Blood Sample Acquisition. De-identified biological samples for device testing were obtained from the blood pathology lab at Robert Wood Johnson University Hospital.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Bioelectronic Sensor with Magnetic Modulation to Quantify Phagocytic Activity of Blood Cells Employing Machine Learning

Norton

Hassan

2022

ACS Sens.

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Microbial infections result in activating an immune response in the human body, which triggers inflammatory pathways resulting in recognition and subsequent killing of the pathogens. Quantifying the blood cells' natural ability to kill pathogens, i.e., phagocytosis, is critical to demonstrating the effectiveness of an individual's response in combating pathogens. Current laboratory processes and equipment that can be used to monitor phagocytic activity are costly and timeconsuming and require significant technical expertise to run such assays. Here, we design and develop a novel biosensing platform capable of quantifying the phagocytic ability of blood cells. The sensor design is composed of electronic sensing and magnetic modulation sub-systems that work in conjunction to monitor phagocytic activity in microfluidic channels. The phagocytes internalize the IgG-coated magnetic beads, and when infused into the sensor, their speed will be modulated using the quadrupole magnetic field configuration as they pass through microfluidic channels where microfabricated electrodes are placed. The electronic sensor will generate the voltage pulse for each passage of the phagocyte, whose distinct features are correlative to the phagocytic activity. We experimentally tested this device using 17 blood samples collected from patients at Robert Wood Johnson Medical Hospital. Further, we developed artificial neural networks (ANN) to improve the accuracy of the phagocytic activity detection. ANN model detected the phagocytic activity with 88.2% accuracy. This novel sensing platform can potentially be used to triage high risk patients and develop personalized theranostics for the septic patients in the future.

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“…To overcome this limitation, Morgan and Spencer proposed a novel multi-planar electrode design for morphological discrimination of non-spherical cells and bioparticles using multiple frequency impedance measurements in a narrow microchannel region, referred to as a microfluidic constriction channel system. 74 The constriction channel design refers to an impedance flow cytometry system containing a narrow region, a relaxing region, and a pair of electrodes. [75][76][77][78][79] The narrow region has dimensions smaller than that of cells.…”

Section: Electrode Engineeringmentioning

confidence: 99%

Section: Bioengineering Approaches For Cell Analysismentioning

confidence: 99%