Biocide and antimicrobial susceptibility of methicillin-resistant staphylococcal isolates from horses

Couto, Natacha; Belas, Adriana; Tilley, Paula; Couto, Isabel; Gama, L. T.; Kadlec, Kristina; Schwarz, Štefan; Pomba, Constança

doi:10.1016/j.vetmic.2013.05.011

Cited by 19 publications

(12 citation statements)

References 13 publications

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“…We estimated the prevalence of lukPQ in an international collection of equid S. aureus isolates (The Netherlands (unpublished), Austria17, the United States18, Sweden19, Portugal20, Italy21 and Spain22) using a PCR assay to identify the three prophage-encoded leukocidins ( lukSF-PV, lukMF’ and lukPQ ). lukPQ was present in 29 out of 194 strains (15%, 95% CI: 10 to 21%) from the Netherlands, Italy and Portugal, whereas lukSF-PV and lukMF’ were only found once and twice, respectively (Supplementary Table 2).…”

Section: Resultsmentioning

confidence: 99%

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Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Koop

Vrieling

Storisteanu

et al. 2017

Sci Rep

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Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (ΦSaeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.

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Section: Resultsmentioning

confidence: 99%

“…To estimate the prevalence of the three phage encoded leukocidins, previously reported collections of horse isolates171820212242 and a selection of isolates from an undescribed Dutch collection were screened by PCR (see Supplementary Methods). …”

Section: Methodsmentioning

confidence: 99%

Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Koop

Vrieling

Storisteanu

et al. 2017

Sci Rep

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“…Mas informam que não foi possível observar co-seleção de resistência a antibióticos de importância em uso clínico. 24,25 Como conclusões deste trabalho, baseado nas evidências obtidas, nas concentrações testadas e controlados os fatores que podem afetar a eficácia de desinfetantes, é possível indicar os compostos químicos hipoclorito de sódio, iodóforo e cloreto de cetil trimetilamônio (quaternário de amônio) para serem usados quando frente a situações-problema sanitários envolvendo MRSA. Também, que os resultados obtidos nesta investigação, assim como nos de outras acima citadas, mostraram não existir evidência da concomitância de resistência dos S. aureus resistentes a meticilina e resistência com os desinfetantes avaliados.…”

Section: Desinfetantesunclassified

Atividade bactericida de três desinfetantes sobre Staphylococcus aureus resistentes a meticilina (MRSA)

Avancini

Both

2017

Rev Epidemiol Control Infect

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Exceto onde especificado diferentemente, a matéria publicada neste periódico é licenciada sob forma de uma licença Creative Commons -Atribuição 4.0 Internacional. http://creativecommons.org/licenses/by/4.0/ R Epidemiol Control Infec, Santa Cruz do Sul, 7(2):85-89, 2017. [ISSN 2238-3360] Please cite this article in press as: AVANCINI, Cesar Augusto Marchionatti; BOTH, Jane Mari Corrêa. Efeito da atividade bactericida de três desinfe-tantes sobre Staphylococcus aureus resistentes a meticilina (MRSA). Revista Justificativa e Objetivos: Os Staphylococcus aureus resistentes a meticilina (MRSA) estão envolvidos em infecções nosocomiais (HA-MRSA), em infecções adquiridas na comunidade (CA-MRSA), nos animais de companhia e em animais para produção de alimentos (LA-MRSA).Na conduta para o controle da sua transmissão, a ação sobre os agentes causais presentes nas superfícies dos ambientes exige atenção, sendo decisiva a escolha de desinfetantes e anti-sépticos. O objetivo deste estudo foi avaliar a atividade bactericida, sobre 21 isolados MRSA e bactéria controle, dos compostos químicos hipoclorito de sódio (HS), iodóforo (I) e quaternário de amônio (QAC -cloreto de cetil trimetilamônio), usados rotineiramente em ambientes hospitalares e de produção e saúde animal, bem como testar hipótese da possibilidade de resistência cruzada entre antibióticos e desinfetantes. Métodos: Pelo teste de suspensão, usando densidade populacional inicial dos inóculos em 10 7 UFC/mL, avaliou-se a atividade bactericida de quatro diluições sucessivas dos desinfetantes, nos tempos de contato de 5, 15 e 30 minutos. Resultados: Observou-se que os desinfetantes nas concentrações HS 25 ppm, I 12,5 ppm e QAC 125 ppm, aos cinco minutos de contato, foram suficientes para inativar a bactéria de referência S. aureus ATCC 6538 e todos os MRSA. Conclusão: Controlados os fatores que interferem na eficácia dos desinfetantes, o hipoclorito de sódio, o iodóforo e o quaternário de amônio são adequados para controlar os MRSA nas fontes de infecção. Nos isolados resistentes ao antibiótico meticilina não foi observada relação de resistência com estes compostos químicos. Descritores: Staphylococcus aureusResistente a Meticilina. Desinfetantes. Higiene. Background and Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) can cause hospital-acquired infections (HA-MRSA), community-acquired ones (CA-MRSA), and infections transmitted by pets and animals raised for food production (livestock-acquired or LA-MRSA). The conduct to control the transmission of these diseases requires a careful action against the causative agents on surfaces in the environment and the choice of disinfectants and antiseptics is crucial. The objective of the present study was to evaluate the effect of the bactericidal activity of sodium hypochlorite (SH), iodophor (I) and a quaternary ammonium compound (QAC), cetyl-trimethyl-ammonium chloride, commonly used in hospital and animal production settings, on 21 MRSA isolates and a control bacterium, and test the hypothesis of cross resistanc...

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“…Selection of disinfectant and antiseptic counter to pathogenic microorganisms is become a problem due to extensive use to check and control the growth of microbes in living and non-living entities. The extensive application of disinfectant and antiseptic products has encouraged the assumption: promotion of resistance in microbes especially in cross resistance to antibiotics [3][4][5][6] . Currently, chemicals are available in the market in the form of disinfectants and antiseptics having ingredients like halogen compounds, alcohol, chlorohexidine, sodium hypochlorite, peroxides, phenols and quaternary ammonium compounds (QAC) such as benzalkonium chloride 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants

Johny¹,

Alkhuzaee²

2019

J Pure Appl Microbiol

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Citation: Bassam Oudh Al-johny and Abdulelah M. Alkhuzaee, Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants, J Pure Appl Microbiol., 2019; 13(4):2065-2072. https://doi.org/10.22207/JPAM.13.4.18 AbstractSuccessive use and misuse of disinfectant against certain microbes may trigger resistance, causing the problem of ineffectiveness of disinfectant due to development of resistance strains. Ultimately, it is speculated to raise public health issues worldwide. Keeping in view the said problem an experiment was designed to test the efficacy of different disinfectants against some pathogenic bacteria isolated from selected restaurants and cafeterias kitchen. A total of 45 isolates were purified and identified. Amongst them, three isolates; Klebsiella pneumoniae, Macrococcus caseolyticus, Staphylococcus sciuri were selected for their pathogenicity while Terribacillus halophilus were selected for significant tolerance to high temperature and salt regimes. Different parameters including Minimum Inhibitory Concentrations (MIC) and Minimum bactericidal concentration (MBC) were determined for disinfectant; sodium hypochlorite, benzalkonium chloride and chloroxylenol. MIC values was: 2500µg/ml to 5000µg/ ml for sodium hypochlorite; 0.54µg/ml to 17.3µg/ml for Benzalkonium chloride; 18.7µg/ml to 150µg/ ml for chloroxylenol. MBC values was: 5000µg/ml to 33320µg/ml for sodium hypochlorite; 8.6µg/ ml to 138µg/ml for Benzalkonium chloride; 75µg/ml to 300µg/ml for chloroxylenol. Conclusively, K.pneumoniae showed high resistance to sodium hypochlorite and benzalkonium chloride compared to other bacteria used in this study. Among the studied, Staphylococcus sciuri were the most tolerant and resistance to chloroxylenol.

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Biocide and antimicrobial susceptibility of methicillin-resistant staphylococcal isolates from horses

Cited by 19 publications

References 13 publications

Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Identification of LukPQ, a novel, equid-adapted leukocidin of Staphylococcus aureus

Atividade bactericida de três desinfetantes sobre Staphylococcus aureus resistentes a meticilina (MRSA)

Isolation and Identification of Pathogenic Bacteria Showing Resistance against Disinfectants

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