Biocompatibility and internalization of molecularly imprinted nanoparticles

Canfarotta, Francesco; Waters, Alicia; Sadler, Robyn; McGill, Paul; Guerreiro, António; Papkovsky, Dmitri B.; Haupt, Karsten; Piletsky, Sergey A.

doi:10.1007/s12274-016-1222-7

Cited by 70 publications

(46 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this apparently simple paradigm requires the tedious task of monitoring several parameters in view of achieving the dream of a highly selective and sensitive "plastic antibody". MIPs can be used in applications where specific chemical recognition is required: specific electrodes, stationary phase of chromatography, extraction materials and nanomedicine [1,[3][4][5]. It is mandatory that cross-linking of the polymer material retains the structural features of the interaction site between the MIP and the target molecule after the later has been removed.…”

Section: Introductionmentioning

confidence: 99%

Design of Molecularly Imprinted Polymeric Materials: The Crucial Choice of Functional Monomers

Anene¹,

Kalfat²,

Chevalier

et al. 2020

Chemistry Africa

View full text Add to dashboard Cite

The choice of the functional monomer is addressed in the present study. It is not only dictated by its ability to interact with the template molecule. Its reactivity towards the cross-linker in the radical polymerization reaction is also to be considered in order to yield a suitable distribution of the monomer bearing binding groups within the material and also to adjust the cross-linking degree which provides rigidity to MIP network. MIPs prepared using two functional monomers of very different reactivity in radical polymerization allowed to investigate the criteria for the optimum choice of the functional monomer. MIPs were made of cross-linked poly(methacrylic acid) or poly(maleic acid) bound as thin films to a silica solid support. Ethylene glycol dimethacrylate was the cross-linker. Calculations of the composition drift of the copolymer material from monomers reactivity ratios give new insights into the control of MIP properties by the choice of the functional monomer. As a consequence of the lower reactivity of maleic acid than methacrylic acid for copolymerization with methacrylic esters, the incorporation of maleic acid is low, the cross-linking density is very high, the polymer coating is very thin, and the specific area is high. The final structure of the MIP network with a predominance of isolated functional units closely surrounded by cross-links yields a rigid material capable of preserving the memory of the model molecule of patulin in molecular imprints. Low reactivity of the functional monomer has beneficial effects regarding the binding selectivity for the target molecule compared to materials prepared from the more reactive methacrylic acid which lead to the formation of flexible polymer formed of short poly(methacrylic acid) sequences.

show abstract

Section: Introductionmentioning

confidence: 99%

Design of Molecularly Imprinted Polymeric Materials: The Crucial Choice of Functional Monomers

Anene¹,

Kalfat²,

Chevalier

et al. 2020

Chemistry Africa

View full text Add to dashboard Cite

show abstract

“…It is also beneficial for the in situ monitoring of biomolecules, since field sensors may need to withstand relatively harsh conditions depending on their designated use. 18 Whilst MIPs are extremely suitable for extraction and quantification of target molecules in complex matrices, their use in biosensors remains limited. This can be explained by two factors; first, difficulties to incorporate MIPs into sensors platforms and, second, lack of detection methods that allow simple interpretation and are suitable for integration into portable devices.…”

Section: Introductionmentioning

confidence: 99%

A novel thermal detection method based on molecularly imprinted nanoparticles as recognition elements

et al. 2018

Self Cite

View full text Add to dashboard Cite

Molecularly Imprinted Polymers (MIPs) are synthetic receptors that are able to selectively bind their target molecule and, for this reason, they are currently employed as recognition elements in sensors. In this work, MIP nanoparticles (nanoMIPs) are produced by solid-phase synthesis for a range of templates with different sizes, including a small molecule (biotin), two peptides (one derived from the epithelial growth factor receptor and vancomycin) and a protein (trypsin). NanoMIPs are then dipcoated on the surface of thermocouples that measure the temperature inside a liquid flow cell. Binding of the template to the MIP layer on the sensitive area of the thermocouple tip blocks the heat-flow from the sensor to the liquid, thereby lowering the overall temperature measured by the thermocouple. This is subsequently correlated to the concentration of the template, enabling measurement of target molecules in the low nanomolar regime. The significant improvement in the limit of detection (a magnitude of three orders compared to previously used MIP microparticles) can be attributed to their high affinity, enhanced conductivity and increased surface-to-volume ratio. It is the first time that these nanosized recognition elements are used in combination with thermal detection, and it is the first report on MIP-based thermal sensors for determining protein levels. The developed thermal sensors have a high selectivity, fast measurement time (<5 min), and data analysis is straightforward, which makes it possible to monitor biomolecules in real-time. The set of biomolecules discussed in this manuscript show that it is possible to cover a range of template molecules regardless of their size, demonstrating the general applicability of the biosensor platform. In addition, with its high commercial potential and biocompatibility of the MIP receptor layer, this is an important step towards sensing assays for diagnostic applications that can be used in vivo.

show abstract

“…Nanoparticles (NPs) smaller than 8 nm are cleared rapidly from the blood stream by the renal system and NPs larger than 200 nm are sequestered by the mononuclear phagocytic system in the liver and spleen[65,66]. NanoMIPs represent an entirely new compound class which can now be deployed to address both extracellular protein targets (as an alternative to biological antibodies), and potentially to currently intractable intracellular proteins[67]. Potentially nanoMIPs can assist with increasing a drug's half-life within the body, increasing drug payload, facilitating targeted drug delivery, improving drug permeability through cell membranes and offering the possibility of oral delivery.One particularly important subject in NP research is the oral delivery of macromolecules.…”

mentioning

confidence: 99%

Molecularly Imprinted Polymers for Cell Recognition

Piletsky

Canfarotta²,

Poma

et al. 2020

Trends in Biotechnology

Self Cite

208

106

View full text Add to dashboard Cite

Since their conception fifty years ago, molecularly imprinted polymers (MIPs) have seen extensive development both in terms of synthetic routes and applications. Perhaps the most challenging target for molecular imprinting are cells. Though early work was based almost entirely around microprinting methods, recent developments shifted towards epitope imprinting to generate MIP nanoparticles. Simultaneously, the development of techniques such as solid phase MIP synthesis have solved many historic issues of MIP production. This review briefly describes various approached used in cell imprinting with a focus on applications of the created materials in imaging, drug delivery, diagnostics and tissue engineering.

show abstract

Biocompatibility and internalization of molecularly imprinted nanoparticles

Cited by 70 publications

References 42 publications

Design of Molecularly Imprinted Polymeric Materials: The Crucial Choice of Functional Monomers

Design of Molecularly Imprinted Polymeric Materials: The Crucial Choice of Functional Monomers

A novel thermal detection method based on molecularly imprinted nanoparticles as recognition elements

Molecularly Imprinted Polymers for Cell Recognition

Contact Info

Product

Resources

About