Biocompatibility of a new cell separator studied by flow cytometry: analyses of platelet antigens during apheresis and storage

Abstract

These studies show that alterations in platelet antigens and platelet activation occur to a small degree during apheresis and storage. These findings demonstrate generally good biocompatibility of this new cell separator.

Binding of activated platelets to WBCs in vivo after transfusion

“…In the Baxter platelet components, initial platelet activation immediately after apheresis was higher than in the COBE components. In contrast, during storage P‐selectin and CD63 expression increased in the COBE apheresis components, while, similar to earlier observations, the neoantigen expression remained stable in the Baxter PCs 19,24 . The final levels of platelet activation in Baxter and COBE apheresis components were comparable.…”

Influence of Storage Time on Activation of Platelets Collected with CS 3000 Plus and Cobe Spectra Using Platelet Storage Containers

“…Additional sensitive factors are neoantigens such as C62p or CD63 that are secreted from internal granules and exposed on the cell surface (15). The upregulation of this receptor on the platelet surface results in the binding of activated platelets to white cells and their subsequent clearance either by the mononuclear phagocyte system or by entrapment of the platelet white cell conjugates in the microcirculation (3). As a consequence, platelet concentrates with a high proportion of activated platelets may have reduced in vivo efficacy.…”

“…One of the most sensitive and specific methods of detecting minimal alterations in platelets and associated microstructures is flow cytometry (2). Antigenic changes at the single cell level can be detected and used for improved quality control and the evaluation of blood–biomedical material interaction (2,3).…”

“…Antigenic changes at the single cell level can be detected and used for improved quality control and the evaluation of blood-biomedical material interaction (2,3).…”

Methods for testing platelet function for transfusion medicine

“…There are well documented differences between apheresis devices and P‐selectin expression of the harvested platelets [33, 34], whereby the initial activation on day 1 was shown to predict the activation values on day 5 of storage [35]. Further, there is good evidence that P‐selectin increases during storage, while the residual ‘activatability’ to express P‐selectin declines [21, 22, 25, 33–38].…”