Background and Objectives: In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells. Material and Methods: Analysis of platelet–leukocyte interaction was performed using multiparameter, three–color flow cytometry. Results: Over the duration of the procedure, there was an increase in the surface expression of CD62p (P–selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly. Conclusion: This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.

These studies show that alterations in platelet antigens and platelet activation occur to a small degree during apheresis and storage. These findings demonstrate generally good biocompatibility of this new cell separator.

This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.

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