Biocompatibility of hyaluronic acid hydrogels prepared by porous hyaluronic acid microbeads

Kim, Jin‐Tae; Lee, Deuk Yong; Kim, Tae-Hyung; Song, Yo-Seung; Cho, Nam-Ihn

doi:10.1007/s12540-014-3022-5

Cited by 29 publications

(26 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, 250 µg mL −1 HA stimulated HaCaT, HUVEC and L929 cell proliferations by 131.0 ± 4.77%, 127.8 ± 3.43% and 133.1 ± 5.75%, respectively. Kim and colleagues demonstrated that hyaluronic acid has no cytotoxic effects on fibroblast cells . Also, Liu and colleagues established that the range 0.01–0.1% HA stimulated cell proliferation …”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial hyaluronic acid production through an alternative extraction method and its characterization

Güngörmedi

Gedikli

Toptaş

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND In this study, hyaluronic acid (HA) was produced using Streptococcus equi ssp. equi via an optimized Central Composite Design process, with purification by an alternative extraction method. Determinations of molecular weight, cytotoxicity, viscosity; proton nuclear magnetic resonance (H‐NMR) and Fourier transform infrared‐attenuated total reflection (FTIR‐ATR) analyses were provided. RESULTS The HA yield obtained was up to c. 12 g L−1 under the reaction conditions of pH 7.8, 33 °C incubation temperature, 24 h incubation time and 187 rpm agitation rate. According to gel permeation chromatography results, HA has an average molecular weight of 79 416 Da. FTIR‐ATR analysis showed that the spectrum of the standard HA sample overlapped with the spectrum obtained from this study. CONCLUSION In this study, higher titer production of HA was obtained with a simple and economical sequential process. Additionally, the results indicate that the HA produced is an appropriate biomaterial for medical applications. © 2019 Society of Chemical Industry

show abstract

Section: Resultsmentioning

confidence: 99%

“…Kim and colleagues demonstrated that hyaluronic acid has no cytotoxic effects on fibroblast cells. 33 Also, Liu and colleagues established that the range 0.01-0.1% HA stimulated cell proliferation. 34…”

Section: Evaluation Of Cytotoxicity and Bioactivity Of Hamentioning

confidence: 99%

Bacterial hyaluronic acid production through an alternative extraction method and its characterization

Güngörmedi

Gedikli

Toptaş

et al. 2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…The pore size was then determined by using the optical microscope equipped with the iSolution Lite image software. [13][14][15][16][17][18][19][20][21] Compressive strength of the irradiated PVA/ CMC hydrogels was examined by using an Instron 5564 with a crosshead speed of 10 mm/min. The specimen with a diameter of 15 mm and a thickness of 5 mm was fabricated and the strength was determined at a point where the specimen thickness decreased by 50%.…”

Section: Hydrogel Synthesismentioning

confidence: 99%

“…The extract test method was conducted on the irradiated PVA/CMC/PEG hydrogels to evaluate the potential of cytotoxicity according to the International Organization for Standardization (ISO 10993-5). [13][14][15][16][17][18][19][20][21] The hydrogels were extracted aseptically in single strength minimum essential medium (1× MEM, Dulbecco's modified Eagle's medium [Gibco, Paisley, United Kingdom] with 10% fetal bovine serum [Gibco, Paisley, United Kingdom] and 1% penicillin-streptomycin) with serum. The ratio of the hydrogels to the extraction vehicle was 0.1 g/ml (ISO 10993-12).…”

Section: Cytotoxicity and Cell Proliferationmentioning

confidence: 99%

Effect of polyethylene glycol molecular weight on cell growth behavior of polyvinyl alcohol/carboxymethyl cellulose/polyethylene glycol hydrogel

Shin

Lee

Kim

et al. 2020

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

Polyvinyl alcohol (PVA)/carboxymethyl cellulose (CMC)/polyethylene glycol (PEG) hydrogel scaffolds are synthesized using cyclic freezing/thawing and subsequent γ‐ray irradiation to evaluate the effect of the molecular weights of PEG (200, 400, 1,000, and 2,000) on strength and cell growth behavior of the hydrogels. As the PEG weight increases from 200 to 2,000, the compressive strength and the pore size decreases gradually from 58.0 ± 8.2 kPa to 17.7 ± 6.1 kPa and from 22.7 ± 3.9 μm to 8.5 ± 1.6 μm, respectively. However, the highest swelling rate is obtained for PVA/CMC/PEG400 hydrogels. The irradiated PVA/CMC/PEG400 hydrogels exhibit tailored properties of the swelling rate of 1,148 ± 34.0%, the compressive strength of 42.5 ± 6.6 kPa, the pore size of 14.6 ± 1.9 μm, and cell viability of 184%. In addition, the fastest L‐929 cell proliferation and growth with time, verified by the cell proliferation (0–48 hr) and the scratch assay (0–15 hr), was observed for the PVA/CMC/PEG400 hydrogels, indicating that they are highly suitable for potential wound dressings that require fast healing regeneration.

show abstract

“…Therefore, in this study, we focused on water‐soluble hyaluronic acid (HA), sodium hyaluronate (Na‐HA), and starch, which are expected to dissolve in the body among polysaccharides . High biocompatibility is reported in guinea pig maximization method, which is a kind of method for examining the biocompatibility of medical materials for HA and Na‐HA . Moreover, starch crops, derived from potato, wheat, corn, and rice, can be mass‐produced at a low cost as raw materials of high production volume.…”

Section: Introductionmentioning

confidence: 99%

Effects of substituents on water vapor sorption in liquid–water‐soluble polysaccharides

Shimanuki

Nagai

2019

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this study, we investigated the influence of the difference in the chemical structures of hyaluronic acid (HA), sodium hyaluronate (Na-HA), and starch water-soluble polysaccharides and the difference in sample shape (as-received samples and membranes) on water vapor solubility. The sorption at this time was characterized by a Fickian diffusion increase at one stage, but HA and starch had a non-Fickian diffusion increase at the two stages in the high humidity region of 52% or more. In addition, all sorption isotherms of HA, Na-HA, and starch did not depend on the sample shape and had an extended dual-mode sorption type behavior consisting of Flory-Huggins and dual-mode sorption types. Furthermore, the infinite dilution solubility coefficient for water molecules increased with increasing surface free energy and correlates with the surface free energy obtained from the contact angle of water of HA, Na-HA, and starch membranes.

show abstract

Biocompatibility of hyaluronic acid hydrogels prepared by porous hyaluronic acid microbeads

Cited by 29 publications

References 13 publications

Bacterial hyaluronic acid production through an alternative extraction method and its characterization

Bacterial hyaluronic acid production through an alternative extraction method and its characterization

Effect of polyethylene glycol molecular weight on cell growth behavior of polyvinyl alcohol/carboxymethyl cellulose/polyethylene glycol hydrogel

Effects of substituents on water vapor sorption in liquid–water‐soluble polysaccharides

Contact Info

Product

Resources

About