Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation

Shishatskaya, Ekaterina I.; Voinova, O. N.; Goreva, A. V.; Mogil'naya, O. A.; Volova, Tatiana G.

doi:10.1007/s10856-007-3345-6

Cited by 84 publications

(76 citation statements)

References 36 publications

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“…The bacteria were cultivated in sterile conditions in a BioFlo 115 cultivator ("New Brunswick", USA) in a volume of 7.5 L using a mineral medium containing glucose [20]. The properties of the polymer have been reported elsewhere [21][22][23][24][25]. Thin films of P3HB were formed by casting a solution of the polymer in chloroform.…”

Section: Introductionmentioning

confidence: 99%

Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma

Syromotina

Surmenev

Boyandin

et al. 2016

Materials Science and Engineering: C

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АbstractThe surface properties of poly-3-hydroxybutyrate (P3HB) membranes were modified using oxygen and an ammonia radio-frequency (RF, 13.56 MHz) plasma. The plasma treatment procedures used in the study only affected the surface properties, including surface topography, without inducing any significant changes in the crystalline structure of the polymer,with the exception being a power level of 250W. Thewettability of themodified P3HB surfaces was significantly increased after the plasma treatment, irrespective of the treatment procedure used. Itwas revealed that both surface chemistry and surface roughness changes caused by the plasma treatment affected surface wettability. A treatment-induced surface aging effect was observed and resulted in an increase in the water contact angle and a decrease in the surface free energy. However, the difference in the water contact angle between the polymers that had been treated for 4 weeks and the untreated polymer surfaces was still significant. A dependence between cell adhesion and proliferation and the polar component of the surface energy was revealed. The increase in the polar component after the ammonia plasma modification significantly increased cell adhesion and proliferation on biodegradable polymer surfaces compared to the untreated P3HB and the P3HB modified using an oxygen plasma.

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Section: Introductionmentioning

confidence: 99%

Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma

Syromotina

Surmenev

Boyandin

et al. 2016

Materials Science and Engineering: C

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“…To the mixture, 3 ml of 0.65M boric acid, 0.5 ml iodine solution and 1.5 ml of distilled water were added. After 15 min incubation, absorbance was measured at 690 nm.Microsphere preparation (1% PVA at 800 rpm) procedure was followed with addition of BSA and gentamicin along with PHB (Shishatskaya ., 2008) Five mg of BSA and gentamicin loaded microspheres were immersed separately in 3 ml of contact lens solution, phosphate buffer (PBS) (pH, 7.2) and simulated body fluid (SBF) (Kokubo ., 1990). The Composition of simulated body fluid consisted pure water, sodium chloride, sodium bicarbonate, potassium chloride, potassium phosphate dibasic trihydrate, magnesium chloride hexahydrate, calcium chloride, Sodium sulphate and Tris base.…”

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confidence: 99%

“…cytotoxicity analysis showed 98% and 95% viability of cells in 3T3L1 cell line for microsphere encapsulated with BSA and gentamicin, respectively. rpm for 15 min and rinsed in distilled water for 7-8 times (Shishatskaya ., 2008).…”

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confidence: 99%

Preparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization

Swornakumari¹,

Meignanalakshmi²,

Legadevi³

et al. 2018

JEB

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Aim :Methodology : Results :Interpretation :Biodegradable poly-3-hydroxybutyrate is one of the most common biopolymer which is used in different fields such as medicine, agriculture, textile, industrial and food packaging. PHB microspheres are useful for targeting drugs to specific infection sites and for prolonged drug release. The present study focus on microsphere preparation for effective controlled drug release using poly-3-hydroxy butyrate biopolymer.In the present study, poly-3-hydroxy butyrate microspheres were prepared using solvent evaporation technique and characterized by SEM and FTIR. Poly-3-hydroxy butyrate microspheres were encapsulated with BSA and gentamicin. Drug encapsulation efficiency was determined. drug release profile and cytotoxicity was also studied.The prepared microspheres were of 2μm in size. Microspheres fabricated with 1% polyvinylalcohol showed encapsulation efficiency of 94.3% and 90.27% with BSA and gentamicin, respectively. The release studies in simulated body fluid, phosphate buffered saline and contact lens solution showed initial burst release followed by controlled release. cytotoxicity analysis showed 98% and 95% viability of cells in 3T3L1 cell line for microsphere encapsulated with BSA and gentamicin, respectively.Poly-3-hydroxy butyrate microspheres were found to release BSA and gentamicin in a controlled manner and were found to be non-toxic by cytotoxicity studies. In vitro in vitro in vitro In vitro in vitroJournal Home page : www.jeb.co.in « E-mail : editor@jeb.co.in Journal of Environmental BiologyPreparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization Surface morphology of PHB microspheres was analyzed using scanning electron microscope (HITACHI model S-3000H).PHB polymer and PHB microspheres were subjected to Fourier Transform Infrared Spectroscopy analysis by FTIR spectrophotometer. Spectra were documented in 4000 cm to 400 cm range Two mg of microspheres were treated with 2 ml of 0.5 M sodium hydroxide for 15 min at 60ºC. To the mixture, 900 of 1 N hydrochloric acid was added to neutralize the samples and the volume was adjusted to 5 ml with distilled water. To the mixture, 3 ml of 0.65M boric acid, 0.5 ml iodine solution and 1.5 ml of distilled water were added. After 15 min incubation, absorbance was measured at 690 nm.Microsphere preparation (1% PVA at 800 rpm) procedure was followed with addition of BSA and gentamicin along with PHB (Shishatskaya ., 2008) Five mg of BSA and gentamicin loaded microspheres were immersed separately in 3 ml of contact lens solution, phosphate buffer (PBS) (pH, 7.2) and simulated body fluid (SBF) (Kokubo ., 1990). The Composition of simulated body fluid consisted pure water, sodium chloride, sodium bicarbonate, potassium chloride, potassium phosphate dibasic trihydrate, magnesium chloride hexahydrate, calcium chloride, Sodium sulphate and Tris base. They were kept in a shaker at 250 rpm at 37ºC. About 1ml of the sample was collected at regular intervals, till 24hrs. Each sample was ...

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“…As a recent example of research in this direction, Da Silva-Valenzuela et al [91] investigated production of nanocomposite microcapsules containing one of two clays for the purposes of drug delivery. This work was built upon earlier suggestions regarding the suitability of PHB microspheres for medical applications [92]. PHB/clay films were cast from chloroform as solvent and microcapsules were produced by a solvent-mediated emulsificationdiffusion technique [93].…”

Section: Summary and Future Trendsmentioning

confidence: 99%

PHA/Clay Nano-Biocomposites

Plackett

2012

Environmental Silicate Nano-Biocomposites

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Polyhydroxyalkanoates or PHAs were first observed in a laboratory in France in the 1920s and, since then, their creation as a form of energy storage in bacteria as well as their practical uses have been much studied. There has been increased interest in commercial production of PHAs in recent times and, over the past 10 years in particular, there have been various reports on solution-cast or melt-processed PHA/clay nano-biocomposites and their properties. In most studies, these nano-biocomposites exhibit intercalated or mixed exfoliated/intercalated morphologies. The use of plate-like clays as additives can lead to some enhancement in the mechanical and gas barrier properties of PHAs as well as increased thermal stability, although the effects depend significantly on clay type, clay organomodifer and process conditions. The influence of clay addition on PHA biodegradability has been either positive or negative according to the particular study. There has been very little research to date on the migration properties of PHA in the context of use in packaging and none yet in which PHA/clay nano-biocomposites have been the focus. If economic and technical challenges can be solved, PHAs should have a promising future in various uses ranging from packaging through to medicine and PHA/clay nano-biocomposites may also play a role in the future by providing a way for PHA material properties to be tuned according to the particular need.

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Biocompatibility of polyhydroxybutyrate microspheres: in vitro and in vivo evaluation

Cited by 84 publications

References 36 publications

Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma

Surface wettability and energy effects on the biological performance of poly-3-hydroxybutyrate films treated with RF plasma

Preparation of microspheres using poly-3-hydroxybutyrate biopolymer and its characterization

PHA/Clay Nano-Biocomposites

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