Biocompatibility of Regular and White MTA and A Grey Portland Cement in Human Dental Pulp Cells

Abstract

Currently many materials have been used with pulpotomies in primary teeth. These include MTA, that as a direct pulp capping material seemed to stimulate natural dentin repair at pulpal exposure sites. The composition of this product is based on Portland cement. Later, white MTA was introduced as an esthetic improvement over the original material (grey MTA). A number of studies have been performed on this new material to determine if it shows the same properties as gray MTA. The biocompatibility of MTA has been previously investigated in vitro using a number of the cell lines. However, the use of primary cultures (in this case pulp fibroblasts) is often desirable. Objective: The objective of this in vitro study was to compare the cytotoxicity in a pulp fibroblasts culture, of regular (grey) MTA, white MTA and Portland cement Material and method: A cytotoxicity study was made in a primary pulp fibroblasts culture of MTA, white and grey, and a grey Portland cement. Results: Without taking into account concentrations, there were no significant differences between regular MTA and white MTA (p=0.146). A significant difference was recorded between grey Portland cement, which were less biocompatible, and regular MTA (p=0.029), and white MTA (p=0.002). Conclusion: The results found in the present study might be an additional argument to support that MTA is biocompatible, and may be considered as a pulp dressing agent in pulpotomized primary molars with carious pulp exposures. However, the grey Portland cement, under the same conditions, shows reduced biocompatibility.

Effect of two different materials in sealing vertical root fractures of intentional replantation on epithelial thickness of periradicular tissue

“…This proved that MTA has high biocompatibility, which can be well tolerated by body tissue compared to self-adhesive resin cement. 10,11 These results are in accordance with a study conducted by Lillo et al 22 and Solanki et al, 23 which reported the biocompatibility of MTA in human cells. In addition, another study reported that the 12-week observation of animal tissue in vitro and in vivo exhibited normal cell growth and no infection.…”