Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications

Abstract

Nitric oxide (NO) is involved in physiological processes, including vasodilatation, wound healing and antibacterial activities. As NO is a free radical, designing drugs to generate therapeutic amounts of NO in controlled spatial and time manners is still a challenge. In this study, the NO donor S-nitrosoglutathione (GSNO) was incorporated into the thermoresponsive Pluronic F-127 (PL) - chitosan (CS) hydrogel, with an easy and economically feasible methodology. CS is a polysaccharide with known antimicrobial properties. Scanning electron microscopy, rheology and differential scanning calorimetry techniques were used for hydrogel characterization. The results demonstrated that the hydrogel has a smooth surface, thermoresponsive behavior and good mechanical stability. The kinetics of NO release and GSNO diffusion from GSNO-containing PL/CS hydrogel demonstrated a sustained NO/GSNO release, in concentrations suitable for biomedical applications. The GSNO-PL/CS hydrogel demonstrated a concentration-dependent toxicity to Vero cells, and antimicrobial activity to Pseudomonas aeruginosa (minimum inhibitory concentration and minimum bactericidal concentration values of 0.5 µg·mL-1 of hydrogel, which corresponds to 1 mmol·L-1 of GSNO). Interestingly, the concentration range in which the NO-releasing hydrogel demonstrated an antibacterial effect was not found to be toxic to the Vero mammalian cell. Thus, the GSNO-PL/CS hydrogel is a suitable biomaterial for topical NO delivery applications.

Influence of Aloe vera and Pluronic F127 on the Enhancement of Photocatalytic Degradation and Antibacterial Activity of ZnO by sol-gel Synthesis Method

“…1a. The stiff and narrow diffraction peaks showed good crystalline structure and purity of the nanoparticles and no other secondary peaks were observed [20]. However, the peak intensity was different for all combinations of ZnO.…”

Quitosanas S-nitrosadas para a liberação térmica e fotoquímica de óxido nítrico

“…Diferentes biomateriais de QT contendo RSNOs têm sido produzidos para a liberação localizada de NO, onde os doadores de NO são incorporados fisicamente por absorção ou quimicamente ligados na estrutura do material. Nesse sentido, os materiais mais comuns são hidrogéis de QT carregados com GSNO [63] ou SNAP [64] . Além disso, já foi reportada a produção de filmes de QT carregados com GSNO [65,66] , bem como o encapsulamento de GSNO [67] e do ácido S-nitrosomercaptosuccínico AMS(SNO) [68] em nanopartículas de QT.…”