Biocompatible Conjugated Polymer Nanoparticles for Efficient Photothermal Tumor Therapy

Geng, Junlong; Sun, Chunyang; Liu, Bin; Liao, Lun-De; Thakor, Nitish V.; Wang, Jun

doi:10.1002/smll.201402092

Cited by 173 publications

(141 citation statements)

References 59 publications

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“…These properties of PDots make them excellent fluorescent probes for many biological applications. For example, they recently have been used for biological detection and imaging 1,9-12 biosensing platforms 13,14 , specific cellular 5 and subcellular targeting and imaging 15 , photoacoustic molecular imaging probes 16 , drug delivery 17 , bioorthogonal labeling 6 and in vivo tumor targeting 9,18 .…”

Section: Introductionmentioning

confidence: 99%

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

White

Jin

et al. 2015

Nanoscale

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The rapid development and acceptance of PDots for biological applications depends on an in depth understanding of their cytotoxicity. In this paper, we performed a comprehensive study of PDot cytotoxicity at both the gross cell effect level (such as cell viability, proliferation and necrosis) and more subtle effects (such as redox stress) on RAW264.7 cells, a murine macrophage cell line with high relevance to in vivo nanoparticle disposition. The redox stress measurements assessed were inner mitochondrial membrane lipid peroxidation (nonyl-acridine orange, NAO), total thiol level (monobromobimane, MBB), and pyridine nucleotide redox status (NAD(P)H autofluorescence). Because of the extensive work already performed with QDots on nanotoxicity and also because of their comparable size, QDots were chosen as a comparison/reference nanoparticle for this study. The results showed that PDots exhibit cytotoxic effects to a much lesser degree than their inorganic analogue (QDots) and are much brighter, allowing for much lower concentrations to be used in various biological applications. In addition, at lower dose levels (2.5 nM to 10 nM) PDot treatment resulted in higher total thiol level than those found with QDots. At higher dose levels (20 nM to 40 nM) QDots caused significantly higher thiol levels in RAW264.7 cells, than was seen with PDots, suggesting that QDots elicit compensation to oxidative stress by upregulating GSH synthesis. At the higher concentrations of QDots, NAD(P)H levels showed an initial depletion, then repletion to a level that was greater than vehicle controls. PDots showed a similar trend but this was not statistically significant. Because PDots elicit less oxidative stress and cytotoxicity at low concentrations than QDots, and because they exhibit superior fluorescence at these low concentrations, PDots are predicted to have enhanced utility in biomedical applications.

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Section: Introductionmentioning

confidence: 99%

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

White

Jin

et al. 2015

Nanoscale

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“…To date, the group has now synthesized CPs exhibiting excellent properties for photothermal therapy [23] and oligomeric material for photodynamic therapy [24].…”

Section: Cpes As Fluorescent Reportersmentioning

confidence: 99%

Poly-and oligothiophenes : Optical probes for multimodal fluorescent assessment of biological processes

Magnusson¹

2015

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IICover: Fibroblasts and melanoma cells stained with a fluorescent thiophene probe called p-HTIm, DAPI and antibodies against ER, proteasomes, α-tubulin, fibronectin, or mitotracker against mitochondria.During the course of the research underlying this thesis, Karin Magnusson was enrolled in Forum Scientium, a multidisciplinary doctoral program at Linköping University, Sweden. ABSTRACTOne interesting class of molecules in the research field of imaging biological processes is luminescent conjugated polythiophenes, LCPs. These fluorescent probes have a flexible backbone consisting of repetitive thiophene units. Due to this backbone, the probes possess unique abilities to give rise to different spectral signatures depending on their target and environment. LCPs are a polydispersed material meaning there is an uneven distribution of lengths of the probe. Recently, monodispersed chemically well-defined material denoted luminescent conjugated oligothiophenes, LCOs, with an exact number of repetitive units and distinct sidechain functionalities along the backbone has been developed. LCOs have the advantages of being smaller which leads to higher ability to cross the blood brain barrier. The synthesis of minor chemical alterations is also more simplified due to the well-defined materials. During my doctoral studies I have used both LCPs and LCOs to study biological processes such as conformational variation of protein aggregates in prion diseases and cellular uptake in normal cells and cancer cells. The research has generally been based on the probes capability to emit light upon irradiation and the interaction with their targets has mainly been assessed through variations in fluorescence intensity, emission-and excitation profiles and fluorescence lifetime decay. These studies verified the utility of LCPs and LCOs for staining and discrimination of both prion strains and cell phenotypes. The results also demonstrated the pronounced influence minor chemical modifications have on the LCO´s staining capacity. IV POPULÄRVETENSKAPLIG SAMMANFATTNING LYSANDE MOLEKYLER FÖR STUDIER AV BIOLOGISKA PROCESSERLuminiscenta konjugerade polytiofener, LCPs, är en väldigt intressant typ av molekyl som kan användas för att studera processer i biologiska system. Strukturen hos dessa fluorescerande molekyler kan liknas vid olika element som är fästa vid en ryggrad. De olika elementen ger molekylen dess affinitet för sin målmolekyl, medan ryggraden är mest ansvarig för molekylens optiska egenskaper. Ryggraden ändrar sin struktur beroende på målmolekylen och resultatet kan avläsas med ett flertal fluorescens-metoder. Längden på ryggraden hos LCPs varierar och varje parti av en LCP består därmed av en blandning längder och storlekar. De senaste åren har Peter Nilssons forskargrupp utvecklat LCPs till molekyler med väldefinierad längd på ryggraden som då betecknas luminiscenta konjugerade oligotiofener, LCOs. Dessa mindre molekyler har större möjlighet att passera blodhjärnbarriären samt att syntesen för små kemiska modifieringar av elementen ...

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“…CPNs can sensitize oxygen molecules under light irradiation to produce ROS and thus be used in PDT 84,85,86 . Moreover, CPNs with strong NIR absorption and high heat conversion efficiency have potential as a new generation of agents for PTT 87 . PDT and PTT hold great promise for non-invasive cancer treatment, and CPN-based image-guided therapy is a burgeoning area of research 87,88,89,90,91,92,93 .…”

Section: Introductionmentioning

confidence: 99%

Conjugated polymer nanomaterials for theranostics

et al. 2017

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Conjugated polymer nanomaterials (CPNs), as optically and electronically active materials, hold promise for biomedical imaging and drug delivery applications. This review highlights the recent advances in the utilization of CPNs in theranostics. Specifically, CPN-based in vivo imaging techniques, including near-infrared (NIR) imaging, two-photon (TP) imaging, photoacoustic (PA) imaging, and multimodal (MM) imaging, are introduced. Then, CPN-based photodynamic therapy (PDT) and photothermal therapy (PTT) are surveyed. A variety of stimuli-responsive CPN systems for drug delivery are also summarized, and the promising trends and translational challenges are discussed.

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Biocompatible Conjugated Polymer Nanoparticles for Efficient Photothermal Tumor Therapy

Cited by 173 publications

References 59 publications

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

Toxicity and oxidative stress induced by semiconducting polymer dots in RAW264.7 mouse macrophages

Poly-and oligothiophenes : Optical probes for multimodal fluorescent assessment of biological processes

Conjugated polymer nanomaterials for theranostics

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