Biocompatible magnetic tris(2-aminoethyl)amine functionalized nanocrystalline cellulose as a novel nanocarrier for anticancer drug delivery of methotrexate

Rahimi, Mahdi; Shojaei, Salman; Safa, Kazem D.; Ghasemi, Zarrin; Salehi, Roya; Yousefi, Bahman; Shafiei‐Irannejad, Vahid

doi:10.1039/c6nj03332f

Cited by 79 publications

(59 citation statements)

References 34 publications

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“…Since the functionalized MNPs are to be used for biomedical applications, the issue of cytotoxicity has to be addressed. In order to investigate the hemocompatibility, the in vitro hemolysis assay was accomplished . Briefly, 1 mL of the human red blood cells (HRBCs) were obtained through removing the serum from the human blood through centrifugation at 3000 rpm for 10 min, then washed four times with sterile PBS solution.…”

Section: Methodsmentioning

confidence: 99%

“…In order to investigate the hemocompatibility, the in vitro hemolysis assay was accomplished. 13,36 Briefly, 1 mL of the human red blood cells (HRBCs) were obtained through removing the serum from the human blood through centrifugation at 3000 rpm for 10 min, then washed four times with sterile PBS solution. The HRBCs were diluted with PBS solution and 0.5 mL of the mixture was mixed with 0.5 mL of sterile deionized water as a positive control, 0.5 mL of PBS as a negative control and 0.5 mL of nanocarrier suspensions at concentration of 10 mg/mL.…”

Section: Biocompatibility Of Nanocarriersmentioning

confidence: 99%

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Enzymatic stimuli‐responsive methotrexate‐conjugated magnetic nanoparticles for target delivery to breast cancer cells and release study in lysosomal condition

Nosrati

Mojtahedi

Danafar

et al. 2018

J Biomedical Materials Res

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In this study, magnetic nanoparticles (MNPs) coated with glycine (F-Gly NPs) and conjugated with methotrexate (MTX) (F-Gly-MTX NPs) were synthesized through a coprecipitation method followed by amidation reaction between the carboxylic acid end groups on MTX and the amine groups on the MNPs surface and studied its cytotoxic effect in vitro. The successful conjugating of MTX onto the nanoparticles (NPs) was confirmed by X-ray diffraction, thermogravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, vibrating sample magnetometer, and transmission electron microscopy techniques. The results showed that the average size was 46.82 ± 5.03 nm. This target drug delivery system is dependent on the release of the MTX within the lysosomal compartment. Hemolysis assay and cytotoxicity study results on HFF-2 and HEK-293 cell lines show that as prepared MNPs are biocompatible. The cytotoxicity of void of the MTX and F-Gly-MTX NPs were compared to each other by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay of the treated MCF-7 cell line. Enzymatic release studies exhibited the release of the MTX via peptide bond cleavage in the presence of proteinase K. These studies specify that the F-Gly-MTX NPs have a very remarkable anticancer effect, for breast cancer cell line. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1646-1654, 2018.

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Section: Methodsmentioning

confidence: 99%

Section: Biocompatibility Of Nanocarriersmentioning

confidence: 99%

Enzymatic stimuli‐responsive methotrexate‐conjugated magnetic nanoparticles for target delivery to breast cancer cells and release study in lysosomal condition

Nosrati

Mojtahedi

Danafar

et al. 2018

J Biomedical Materials Res

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“…Cellular uptake of PB NPs was higher than TB NPs due to the smaller size compared to TB NPs. TB NPs uptake at 0.5 h was higher than PB NPs maybe due to the lower negative charge of TB (−9.25) compared to PB (−17.5) leads to more intracellular uptake at first but throughout the time size of NPs played decisive role in intracellular absorption 33 .…”

Section: Discussionmentioning

confidence: 94%

“…NPs prepared by USH method indicated acceptable negative surface charge, lower PDI values and smaller particle size than RSH methods. Lower PDI values indicate the uniformity of size in the synthesized particles 32,33 . The PDI values of both TB and PB NPs which prepared by USH method were below 0.29 but in RSH method ranged from 0.68-0.8.…”

Section: Discussionmentioning

confidence: 99%

Novel nano-vehicle for delivery and efficiency of anticancer auraptene against colon cancer cells

Jalilzadeh

Samadi

Salehi

et al. 2020

Sci Rep

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The aim of this study is to devise, prepare and characterize nano encapsulated auraptene (AUR) and evaluate cytotoxic and apoptotic effects on HT-29 colon cancer cells. Herein, AUR nano formulations were prepared by triblock (PCL-PEG-PCL) and pentablock (PLA-PCL-PEG-PCL-PLA) biodegradable copolymers in order to increase AUR bioavailability as an anticancer agent. The preparation of nano particles (NPs) was done with rotor stator homogenization (RSH) and Ultrasonic homogenization (USH) methods. The physicochemical characteristics of prepared nanoparticles (NPs) were studied using HNMR, FTIR, GPC, DLS and SEM techniques. The smaller hydrodynamic size (110 nm) and polydispersity index (PDI: 0.288) as well as higher cellular uptake (89%) were observed in PB NPs rather than TB NPs. The highest cytotoxic and apoptotic effects were observed in AUR loaded PB NPs compared to AUR loaded TB NPs and free AUR obtained by MTT assay, cell cycle arrest, Annexin V-FITC, DAPI staining and RT-PCR techniques. Real time PCR results indicated that Bax /Bcl2 expression ratio as an apoptosis predicting criterion, in free AUR, AUR loaded TB and AUR loaded PB have increased 6, 9 and 13 times, respectively (p value < 0.05). In conclusion, using biodegradable nano-vehicles for sustained delivery of natural anti-cancer compounds may open new perspectives for treatment of cancer patients. NP preparation and characterization. Nano particles size, zeta potential and morphology. Table 1 summarized the size distribution, polydispersity index and zeta potential of TB and PB NPs prepared by RSH and USH methods, measured by DLS technique. Blank PB and TB NPs size prepared by RSH method were 170 and 308 nm, while size of NPs prepared by USH method were 109.8 and 314.5 nm, respectively. PDI values of PB and TB NPs prepared by RSH method were 0.805 and 0.675 while, this value for NPs prepared by USH methods were 0.288 and 0.192, respectively (Figs. S3-S10). Surface charge of TB and PB NPs which were prepared by RSH method were −26.4 mV and −19.6 mV and for TB and PB NPs which were prepared by USH method were −17.5 mV and −9.26 mV. Due to the superiority of USH compare to RSH method in terms of smaller size, narrower PDI and lower negative surface charge, we used USH method in rest of this study. Figures 5 and 6 show the SEM images of TB and PB NPs prepared by both RSH and USH methods. As it has been seen in the SEM images revealed that NPs real size were in the range of 10 nm to 50 nm also NPs had homo-dispersed spherical morphology. Scientific RepoRtS |(2020) 10:1606 | https://doi. Auraptene release.In the current study, the release of AUR from TB and PB NPS was studied over 120 h and the controlled-release potential was assessed (Fig. 7). The release data generated in this work indicated that in pH 7.4 environment over 24 h only 16.74% and 14.29% of the drug were released from AUR loaded PB and AUR loaded TB NPs respectively. Which in pH 5.4 environment AUR release from AUR loaded PB and AUR loaded TB NPs at the same time were 14.39% and 12.76% respe...

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“…In order to examine the blood biocompatibility, the in vitro hemocompatibility assay was done . The human blood was centrifuged at 3000 rpm for 8 min and washed five times with sterile saline solution to eliminating the serum and gain of human red blood cells.…”

Section: Materials and Proceduresmentioning

confidence: 99%

Preparation and characterization of magnetic theranostic nanoparticles for curcumin delivery and evaluation as MRI contrast agent

Nosrati

Charmi

Abedini

et al. 2018

Applied Organom Chemis

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In this study we described the production of curcumin (CUR) loaded L-tyrosine (Tyr coated Fe 3 O 4 magnetic nanoparticles. CUR loaded Tyr modified Fe 3 O 4 nanoparticles (F@Tyr@CUR NPs) were analyzed by transmission electron microscopy, fourier transform infrared spectroscopy, thermo gravimetric analysis, vibrating sample magnetometer, X-ray diffraction and dynamic light scattering techniques. The dynamic light scattering results showed that the ζ-potential of F@Tyr@CUR NPs was about −15.4 mV. Also, the internal average size was about 25.81 ± 3.31 (mean ± SD (n = 30)) nm. The drug loading and encapsulation efficiency were 8.87 ± 0.09% and 35.21 ± 0.41%, respectively. In addition, hemolysis test on human red blood cells were conducted to determine the hemocompatibility of F@Tyr@CUR NPs. Next, MTT assays on HEK-293 were conducted to determine the toxicity of F@Tyr@CUR NPs.Lastly, the potential anticancer effect of F@Tyr@CUR NPs was considered on MCF-7 cancer cell line. The outcomes indicated that these F@Tyr@CUR NPs are appropriate for delivery of CUR anticancer drug. Also, the MRI training established the effectiveness of Fe 3 O 4 magnetic nanoparticles as contrast agent for the revealing of tumor as evidenced from the phantom images as well as higher T 2 relaxivity.

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Biocompatible magnetic tris(2-aminoethyl)amine functionalized nanocrystalline cellulose as a novel nanocarrier for anticancer drug delivery of methotrexate

Abstract: A smart, pH-responsive and biocompatible nanocarrier, aimed to achieve an efficient targeted drug delivery system, was facilely synthesized.

Cited by 79 publications

References 34 publications

Enzymatic stimuli‐responsive methotrexate‐conjugated magnetic nanoparticles for target delivery to breast cancer cells and release study in lysosomal condition

Enzymatic stimuli‐responsive methotrexate‐conjugated magnetic nanoparticles for target delivery to breast cancer cells and release study in lysosomal condition

Novel nano-vehicle for delivery and efficiency of anticancer auraptene against colon cancer cells

Preparation and characterization of magnetic theranostic nanoparticles for curcumin delivery and evaluation as MRI contrast agent

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