2022
DOI: 10.1371/journal.pone.0262950
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Biocompatible micro tweezers for 3D hydrogel organoid array mechanical characterization

Abstract: This study presents novel biocompatible Polydimethylsiloxane (PDMS)-based micromechanical tweezers (μTweezers) capable of the stiffness characterization and manipulation of hydrogel-based organoids. The system showed great potential for complementing established mechanical characterization methods such as Atomic Force Microscopy (AFM), parallel plate compression (PPC), and nanoindentation, while significantly reducing the volume of valuable hydrogels used for testing. We achieved a volume reduction of ~0.22 μl… Show more

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Cited by 5 publications
(2 citation statements)
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“…Pure collagen hydrogels were prepared using nine parts of collagen and one part of 10X PBS, adjusting the pH of the mixture to 7.4 with NaOH 0.1 N [21]. The collagen mixture and all reagents were kept at 4 • C to prevent gelation.…”
Section: Fabrication Of 3d Collagen Hydrogelsmentioning
confidence: 99%
“…Pure collagen hydrogels were prepared using nine parts of collagen and one part of 10X PBS, adjusting the pH of the mixture to 7.4 with NaOH 0.1 N [21]. The collagen mixture and all reagents were kept at 4 • C to prevent gelation.…”
Section: Fabrication Of 3d Collagen Hydrogelsmentioning
confidence: 99%
“…Further experimental advances to characterize cell spheroids mechanical behavior, such as the Young's modulus and characteristic relaxation times, include other techniques, such as isotropic compression by osmotic pressure, [26,27] atomic force microscopy (AFM), [28,29] micro-indentation, [30] tweezers, [31] cavitation rheology [32,33] and elastography. [34,35] Each of these methods has its own strengths and limitations, and their results, in terms of Young's modulus, can vary, depending on the specific technique used, as shown in Table S1 (Supporting Information) for different immortalized cell lines and human biopsies.…”
Section: Introductionmentioning
confidence: 99%