Biocompatible polyhydroxybutyrate (PHB) production by marine Vibrio azureus BTKB33 under submerged fermentation

Sasidharan, Raghul Subin; Bhat, Sarita G.; Chandrasekaran, M.

doi:10.1007/s13213-014-0878-z

Cited by 41 publications

(35 citation statements)

References 39 publications

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“…like V. azureus exhibited maximum PHA production at 15 g l −1 NaCl (Sasidharan et al . ). The potential of the isolate MCCB 284 to utilize different carbon sources for the PHB production was examined and glycerol supported the maximum biomass production (3 g l −1 ) and PHB yield (68%) based on CDW.…”

Section: Discussionmentioning

confidence: 97%

Production and characterization of polyhydroxybutyrate fromVibrio harveyiMCCB 284 utilizing glycerol as carbon source

et al. 2016

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Section: Discussionmentioning

confidence: 97%

Production and characterization of polyhydroxybutyrate fromVibrio harveyiMCCB 284 utilizing glycerol as carbon source

et al. 2016

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“…The lowest growth rate was achieved at static condition, while a significant increase in growth rate accompanied by accumulation of PHA was gradually increased by increasing agitation rate up to 200 rpm after 36 h. This might be attributed to the increase in oxygen transfer and mass transfer rates due to increased surface area of contact among media components [26]. Beyond this level, higher growth was achieved but accumulation of PHA was decreased that attributed to limitations of stress conditions required for PHA granules storage [31].…”

Section: Discussionmentioning

confidence: 93%

“…Inorganic chemicals such as ammonia, or ammonium salts are an important for maximizing biomass concentration and PHA accumulation [23]. Ammonium chloride was reported as the best nitrogen source for PHAs production by several researchers [17,[24][25][26]. Kalaiyezhini and Ramachandran [27] found that ammonium sulphate supported high growth and PHA production from glycerol in Paracoccus denitrificans.…”

Section: Discussionmentioning

confidence: 99%

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

2017

J App Biol Biotech

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Polyhydroxyalkalonates (PHAs) are biodegradable thermoplastics that are receiving immense attention as an alternative to petroleum derived plastics. The present study aimed to produce PHA using cheap and ecofriendly biodiesel waste glycerol as a substrate. Several PHA-producing bacterial isolates were isolated from different environmental samples and their efficacy for PHA production was assessed. Isolate Azu-IN1 showed the highest PHA production and was identified as Zobellella taiwanensis Azu-IN1 using 16S rRNA gene sequence and biochemical characterization. Factors affecting PHA production were optimized in batch fermentations. Supplementation of ammonium chloride as nitrogen source, methanol as an auxiliary carbon source, and agitation rate are the main limiting factors affecting PHA production. Maximum PHA production of 2.65 g/L at recovery yield of 50.3 (%), w/w) was achieved after 36 h in batch fermentation using optimized medium. Enhanced PHA production of 3.73 g/L with recovery yield of 61.7 % (w/w) was obtained in fed batch fermentation. The characteristics of extracted PHA were analyzed using Fourier Transform Infrared Spectroscopy (FTIR) 1 H and 13 C Nuclear Magnetic Resonance (NMR) spectroscopy. This is the first report on accumulation of PHA by Zobellella taiwanensis using glycerol as the sole carbon source.

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“…Three different enzymes are involved in PHB biosynthesis pathway (fi g. 4). New phaC genes that encode PHA synthase enzymes, produce PHAs with novel monomer compositions, have been isolated from different bacteria in various environments (Sasidharan et al, 2016;Ling et al, 2011;Ng & Sudesh, 2016)Malaysia. It is a rod-shaped, gram-negative bacterium with high sequence identity (99%.…”

Section: Genes and Enzymes Involved In Bacterial Phb Synthesis Pathwaymentioning

confidence: 99%

Recent updates on the economic use of poly hydroxy butyrate (PHB): A green alternative to plastics

Jangra¹,

Ikbal²,

Pippal³

et al. 2018

Biosci. Biotech. Res. Comm

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Excessive uses of polymer plastics in boundless spheres have resulted in waste accumulation. There is an urgent need for switching over to compostable and biodegradable plastics that too from renewable resources. Polyhydroxybutyrate (PHB) is a unique biopolymer which is biodegradable and most close to plastics which is miscible with the conventional plastic. The adaptation and commercialization of PHB depend upon its ready availability in a pocketfriendly manner. The following presentation attempts to discuss economic strategies to reduce the production costs of PHB as well as its expeditious production from Agri-byproducts and its applications in various fi elds. Various sources like microorganisms, genetically modifi ed bacteria, plants and different methods are being looked into, so that quality, quantity and fi nancial side of PHB production can be controlled. It is presented here that PHB production is a natural capability of microbes exploited recently. This potential in the form of new technology is contributing to fulfi l the need of plastic along with the environmental beauty and cleanliness. Novel bacterial fermentation strategies of polyhydroxybutyrate production, for improved productivity and quality of PHB for commercial production are summarized here.

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Biocompatible polyhydroxybutyrate (PHB) production by marine Vibrio azureus BTKB33 under submerged fermentation

Cited by 41 publications

References 39 publications

Production and characterization of polyhydroxybutyrate fromVibrio harveyiMCCB 284 utilizing glycerol as carbon source

Production and characterization of polyhydroxybutyrate fromVibrio harveyiMCCB 284 utilizing glycerol as carbon source

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

Recent updates on the economic use of poly hydroxy butyrate (PHB): A green alternative to plastics

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