Biocompatible silver(I) complexes with heterocyclic thioamide ligands for selective killing of cancer cells and high antimicrobial activity – A combined in vitro and in silico study

N‐Heterocyclic Carbene (NHC) Silver Complexes as Versatile Chemotherapeutic Agents Targeting Human Topoisomerases and Actin

“…The 1 H NMR spectrum of 2 (CDCl 3 ) shows one singlet for the methyl hydrogens of the acetate ligand at 2.01 ppm; accordingly, in 13 C NMR singlet for the carbonyl carbon at 179.0 ppm and at 23.0 ppm for the methyl carbon of the acetate ligand have been observed, whereas the carbenic carbon was observed at 179.5 ppm. For complex 4, the 1 H NMR spectrum, (DMSO-d 6 ) shows singlet for the protons of methyl of the acetate ligand at 1.98 ppm; in 13 C NMR the carbenic carbon was assigned at 182.7 ppm, whereas the carbonyl carbon is at 173.4 ppm and the methyl carbon of the acetate ligand is at 24.1 ppm. In the 1 H NMR spectra in DMSO-d 6 of the complexes 5 and 6 the signal at 1.73 ppm is attributable to the methyl group.…”

“…Silver complexes were successfully characterized using 1 H and 13 C{ 1 H} NMR spectroscopy and mass The success of the counterion exchange reaction was confirmed, in the 1 H and 13 C spectra, by the presence of signals attributable to the acetate anion. The 1 H NMR spectrum of 2 (CDCl 3 ) shows one singlet for the methyl hydrogens of the acetate ligand at 2.01 ppm; accordingly, in 13 C NMR singlet for the carbonyl carbon at 179.0 ppm and at 23.0 ppm for the methyl carbon of the acetate ligand have been observed, whereas the carbenic carbon was observed at 179.5 ppm. For complex 4, the 1 H NMR spectrum, (DMSO-d 6 ) shows singlet for the protons of methyl of the acetate ligand at 1.98 ppm; in 13 C NMR the carbenic carbon was assigned at 182.7 ppm, whereas the carbonyl carbon is at 173.4 ppm and the methyl carbon of the acetate ligand is at 24.1 ppm.…”

“…[22] The complexes are soluble in polar solvents, such as dimethyl sulfoxide and chloroform; they are stable in air and towards moisture. Silver complexes were successfully characterized using 1 H and 13 C{ 1 H} NMR spectroscopy and mass The success of the counterion exchange reaction was confirmed, in the 1 H and 13 C spectra, by the presence of signals attributable to the acetate anion. The 1 H NMR spectrum of 2 (CDCl 3 ) shows one singlet for the methyl hydrogens of the acetate ligand at 2.01 ppm; accordingly, in 13 C NMR singlet for the carbonyl carbon at 179.0 ppm and at 23.0 ppm for the methyl carbon of the acetate ligand have been observed, whereas the carbenic carbon was observed at 179.5 ppm.…”

“…In the 1 H NMR spectra in DMSO-d 6 of the complexes 5 and 6 the signal at 1.73 ppm is attributable to the methyl group. In the 13 For all complexes, the elemental analysis gives the predictable composition in C, H, N, whereas their structure in the gas phase can be elucidated by mass spectrometry. In fact, ESI or MALDI spectra show the peaks due to [Ag(NHC) 2 ] + species.…”

“…These complexes showed a good antiplasmodial properties on chloroquine‐resistant P. falciparum parasite and an in vitro antiproliferative activity towards MDA‐MB‐231 and MCF‐7 breast cancer cell lines, as well as HT‐29 colon cancer cell line [12] . Moreover, Ag(I) complexes with heterocyclic thioamides and phosphine ligands exhibited high in vitro antimicrobial potency and cytotoxicity on HeLa and MCF‐7 cancer cells [13] . Recently the anticancer activity of NHC silver complexes is receiving also rising interest [14] .…”