Bioconjugated gold nanoparticles as an efficient colorimetric sensor for cancer diagnostics

Akshaya, K. B.; Arthi, C.; Pavithra, A; Poovizhi, P.; Antinate, S. Shilpa; Hikku, G.S.; Jeyasubramanian, K.; Murugesan, Ramachandran

doi:10.1016/j.pdpdt.2020.101699

Cited by 40 publications

(24 citation statements)

References 99 publications

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“…Typically, metal NPs (Ag, Au, Cu, and Pt) exhibit SPR behavior. Easily tunable physicochemical properties and biocompatibility make AuNPs excellent nano‐sensing probes (Figure 6a; Akshaya et al, 2020). Over the years, this technique has been used to detect several cancers such as leukemia, oral cancer, lung cancer, exosomes from ovarian cancer, and tumor‐specific CD8+ T Cells (Im et al, 2014; Ribaut et al, 2016; Soler et al, 2018; Valsecchi et al, 2016).…”

Section: Nano‐biosensors: a Step Towards Advanced Detection Platformsmentioning

confidence: 99%

Nano‐diagnostics as an emerging platform for oral cancer detection: Current and emerging trends

Chakraborty

Ghosh

Kumar

et al. 2022

WIREs Nanomed Nanobiotechnol

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Globally, oral cancer kills an estimated 150,000 individuals per year, with 300,000 new cases being diagnosed annually. The high incidence rate of oral cancer among the South-Asian and American populations is majorly due to overuse of tobacco, alcohol, and poor dental hygiene. Additionally, socioeconomic issues and lack of general awareness delay the primary screening of the disease. The availability of early screening techniques for oral cancer can help in carving out a niche for accurate disease prognosis and also its prevention. However, conventional diagnostic approaches and therapeutics are still far from optimal. Thus, enhancing the analytical performance of diagnostic platforms in terms of specificity and precision can help in understanding the disease progression paradigm. Fabrication of efficient nanoprobes that are sensitive, noninvasive, cost-effective, and less labor-intensive can reduce the global cancer burden. Recent advances in optical, electrochemical, and spectroscopy-based nano biosensors that employ noble and superparamagnetic nanoparticles, have been proven to be extremely efficient. Further, these sensitive nanoprobes can also be employed for predicting disease relapse after chemotherapy, when the majority of the biomarker load is eliminated. Herein, we provide the readers with a brief summary of conventional and new-age oral cancer detection techniques. A comprehensive understanding of the inherent challenges associated with conventional oral cancer detection techniques is discussed. We also elaborate on how nanoparticles have shown tremendous promise and effectiveness in radically transforming the approach toward oral cancer detection.

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Section: Nano‐biosensors: a Step Towards Advanced Detection Platformsmentioning

confidence: 99%

Nano‐diagnostics as an emerging platform for oral cancer detection: Current and emerging trends

Chakraborty

Ghosh

Kumar

et al. 2022

WIREs Nanomed Nanobiotechnol

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“…The aptasensor was able to quantify the bacteria with a detection limit of 4.5 × 10 3 cfu/mL, and the developed sensor was acceptable for the detection of E. coli in skim-milk samples. Apart from light signals, colorimetric analysis is a simple and low-cost technique based on direct observation of color change by the naked eye [ 76 ] or measurement using a spectrophotometer [ 13 ]. A colorimetric assay was used in aptasensors for microbial detection of Salmonella typhimurium [ 77 ], Staphylococcus aureus [ 78 ] and Shigella flexneri [ 79 ].…”

Section: Aptamer-based Biosensorsmentioning

confidence: 99%

Advances in Aptamer-Based Biosensors and Cell-Internalizing SELEX Technology for Diagnostic and Therapeutic Application

et al. 2022

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Aptamers are a group of synthetic single-stranded nucleic acids. They are generated from a random library of single-stranded DNA or RNA by a technology named systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a repetitive process to select and identify suitable aptamers that show high affinity and specificity towards target cells. Great strides have been achieved in the design, construction, and use of aptamers up to this point. However, only a small number of aptamer-based applications have achieved widespread commercial and clinical acceptance. Additionally, finding more effective ways to acquire aptamers with high affinity remains a challenge. Therefore, it is crucial to thoroughly examine the existing dearth and advancement in aptamer-related technologies. This review focuses on aptamers that are generated by SELEX to detect pathogenic microorganisms and mammalian cells, as well as in cell-internalizing SELEX for diagnostic and therapeutic purposes. The development of novel aptamer-based biosensors using optical and electrical methods for microbial detection is reported. The applications and limitations of aptamers are also discussed.

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“…Colorimetric-based aptasensors have been used for the detection of disease biomarkers, due to their simplicity, ease of use, accessibility, and point-of-care detection (65,66). The colorimetric method is a promising technique due to the possibility of detection by simply visual color change (67). Xu et al developed a highly sensitive colorimetric-based aptasensor for the detection of exosomes obtained from breast and pancreatic cancer cells.…”

Section: Colorimetric Aptasensorsmentioning

confidence: 99%

Advances in Optical Aptasensors for Early Detection and Diagnosis of Various Cancer Types

Zahra

Khan

2021

Front. Oncol.

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Cancer is a life-threatening concern worldwide. Sensitive and early-stage diagnostics of different cancer types can make it possible for patients to get through the best available treatment options to combat this menace. Among several new detection methods, aptamer-based biosensors (aptasensors) have recently shown promising results in terms of sensitivity, identification, or detection of either cancerous cells or the associated biomarkers. In this mini-review, we have summarized the most recent (2016–2020) developments in different approaches belonging to optical aptasensor technologies being widely employed for their simple operation, sensitivity, and early cancer diagnostics. Finally, we shed some light on limitations, advantages, and current challenges of aptasensors in clinical diagnostics, and we elaborated on some future perspectives.

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Bioconjugated gold nanoparticles as an efficient colorimetric sensor for cancer diagnostics

Cited by 40 publications

References 99 publications

Nano‐diagnostics as an emerging platform for oral cancer detection: Current and emerging trends

Nano‐diagnostics as an emerging platform for oral cancer detection: Current and emerging trends

Advances in Aptamer-Based Biosensors and Cell-Internalizing SELEX Technology for Diagnostic and Therapeutic Application

Advances in Optical Aptasensors for Early Detection and Diagnosis of Various Cancer Types

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