Biocontrol of Bayoud Disease (Fusarium oxysporum F. Sp. Albedinis) on Deglet-nour Variety of Date Palm (Phoenix dactylifera L.) in South Western Oases of Algeria

Abstract

Background: This work has the objective to biocontrol the bayoud disease of date palm caused by the fungus Fusarium oxysporum f. sp. albedinis (Foa), is the major disease in Algerian palm groves of south western region. This in vivo biocontrol was done on date palm variety Deglet-Nour, using two antagonistic fungi species, Trichoderma harzianum and Aspergillus flavus.Methods: Twenty Foa isolates obtained from isolation of the spines carrying the typical symptoms of Bayoud disease were used. Two strains T. harzianum and A. flavus, were isolated from rhizosphere soils of the date palm trees. Using the greenhouse screening test, which was carried out on 3-4 month-old date palm seedlings at the rate of 5 ml of inoculum suspension.Result: A statistical analysis showed a significant (P less than 0.05), difference of Deglet-Nour seedlings reactions against Foa isolates and antagonistic fungi were observed. All date palm trees present susceptibility against Foa until the total mortality of seedlings. The biocontrol test showed that two antagonists showed different reaction, with the complete resistance for seedlings treated by T. harzianum with the mortality rate (rm%) reduced at 100%, while, the second antagonist A. flavus reduced the rm% at 25%. We can apply of these antagonistic fungi to protect our groves contaminated by Bayoud disease and also contain this susceptible commercial variety.

An Insight into All Tested Small Molecules against Fusarium oxysporum f. sp. Albedinis: A Comparative Review

“…After some modifications, the agar diffusion approach is used for the antifungal analysis of pyrazole-and imidazole-based derivatives. In short, after isolation and preparation of the Fusarium fungus, the sterilized solution of the six compounds tested (1)(2)(3)(4)(5)(6) in dimethyl sulfoxide (DMSO) is mixed with the potato dextrose agar (PDA) medium as an emulsifier at different concentrations using the method mentioned in the literature [16]. These compounds were synthesized by Takfaoui et al using direct diarylation of pyrazoles and imidazoles with aryl halides, using palladium as the catalyst, DMAc as the solvent, and CsOAc as the base [64,65].…”

“…Bayoud disease [1][2][3][4][5], caused by the telluric fungus pathogen Fusarium oxysporum f. sp. albedinis (F.o.a) [6][7][8][9], represents the leading dangerous agent of date palms cultivation, having killed more than 15 million Moroccan and Algerian date palm trees.…”