Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists

Shi, Cuijuan; Yan, Peisheng; Li, Jiafei; Wu, Hanqi; Li, Qianwei; Guan, Shanshan

doi:10.3390/ijerph110101094

Cited by 61 publications

(27 citation statements)

References 29 publications

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“…The effect of EOs on the growth of Fusarium fungi on wheat was investigated using the method described by Shi et al (2014) with some modifications. Each EO solution (5 cm 3 ) was mixed with 25 g of sterile wheat grain in an Erlenmeyer flask.…”

Section: Methodsmentioning

confidence: 99%

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

et al. 2019

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Essential oils (EOs) are products of plant origin and include mixtures of different chemical compounds. These volatile substances have many interesting properties, including antifungal properties. Fungi may develop under field conditions on crops such as wheat or corn and are able to synthesize mycotoxins, which adversely affect livestock and human health. In the present study, selected EOs were used to inhibit the growth of Fusarium graminearum and F. culmorum and reduce the concentrations of mycotoxins in wheat grain. The EOs significantly inhibited the growth of tested Fusarium species (90.99–99.99%), as determined based on ergosterol quantitative analysis. Only the addition of orange oil to F. culmorum exhibits a different inhibition capacity (68.13%). EO application resulted in a large reduction in zearalenone content (99.08–99.99%); only in the case of orange oil application was the reduction estimated at approximately 68.33%. However, all EOs provided a significant reduction in the concentration levels of group B trichothecenes (94.51–100%). It can be concluded that EOs inhibit the growth of fungi of the genus Fusarium and reduce concentration levels of the mycotoxins zearalenone and group B trichothecenes.

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Section: Methodsmentioning

confidence: 99%

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

et al. 2019

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“…strains, Pediococcus pentosaceus strains, and metabolites Lactobacillus rhamnosus RC007 and Lactobacillus plantarum JM113. Shi et al (2014) screened 32 bacteria strains from peanut shells and investigated their ability to inhibit the growth of F. graminearum mycelia and DON production in vitro. All strains exhibited the ability to inhibit the growth of F. graminearum, and the suppression rate of 62.5% of them exceeded 40% after incubation for 4 days.…”

Section: Lactic Acid Bacteriamentioning

confidence: 99%

Deoxynivalenol: Masked forms, fate during food processing, and potential biological remedies

Guo

Wang

et al. 2020

Comp Rev Food Sci Food Safe

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Deoxynivalenol (DON) has drawn global attention because of its prevalence and significant effects on human or animal health. Biological remedies for DON have been developed from preharvest to postharvest. Applying microbes, including bacteria, fungi (yeast and molds), and enzymes, results in inhibited synthesis, structural destruction, or adsorption of DON. DON can be degraded into masked forms by phase I metabolism or phase II metabolism. During food processing, DON content changes dynamically and is even transformed. Physical, chemical, thermal, or biological processes physically reduce DON content. Temperature, heating time, enzymes, food additives, microorganisms, food composition, contamination level, and other ingredients are key factors. Although DON content can be reduced during food processing, increases in other toxins, such as DON‐3‐β‐d‐glucoside and 3‐acetyl‐DON, can be potentially risky. The application of biodegradation methods in food processing bears research significance. Both microorganisms and enzymes can be potentially used. Novel techniques, such as RNA interference, omics technologies, or enzymes coupled with the genetic engineering method, can be introduced. This review systematically updates the understanding of masked forms of DON, biological degradation strategy, fate of DON during processing, and future trends for biodegradation. Challenges to the successful application of biological methods may include the stability and suitability of the detoxification agents, security of degradation products, and successful application for industrial production.

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“…Then inoculated with spore suspension from 7 days agar slant using 0.05% tween 80. The incubation of starter was done at room temperature for 7 days, and later dried then ground into rough powder, so the starter powder was ready to be used for inoculation (Sardjono et al, 2004 b ;Sardjono 2008;Shi et al, 2014).…”

Section: Microorganisms and Preparation Of Starter Culturementioning

confidence: 99%

Local strains Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1 as a reducing and detoxifying agents for deoxynivalenol

Arifin¹,

Setyabudi²,

Sardjono³

2019

MJM

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Aims: Deoxynivalenol is a type B trichothecene produced by Fusarium graminearum that can cause serious health problems in human and livestock. The present study aimed to reduce and detoxify deoxynivalenol using a local strain Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1. Methodology and results: Corn as solid substrate artificially inoculated with F. graminearum bio 163252 to produce deoxynivalenol. Deoxynivalenol contaminated corn then inoculated with A. oryzae KKB4 and R. oryzae KP1R1. During fermentation, a decrease in deoxynivalenol levels is analyzed including loss of dry matter and glucosamine content. Deoxynivalenol was extracted from the substrate by solid phase extraction and quantified using high-performance liquid chromatography. The reduction of deoxynivalenol by A. oryzae KKB4 and R. oryzae KP1R1 were 65.91% and 56.82%, respectively after ten days of fermentation. Toxicity analysis revealed that residues of deoxynivalenol were not toxic to growth of Saccharomyces cerevisiae cells. Conclusion, significance and impact of study: Local strains A. oryzae KKB4 and R. oryzae KP1R1 were able to reduce and detoxify deoxynivalenol in solid substrates. This study provides supporting data to control mycotoxin that is critical for food and feed safety.

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Biocontrol of Fusarium graminearum Growth and Deoxynivalenol Production in Wheat Kernels with Bacterial Antagonists

Cited by 61 publications

References 29 publications

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

Deoxynivalenol: Masked forms, fate during food processing, and potential biological remedies

Local strains Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1 as a reducing and detoxifying agents for deoxynivalenol

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