Biocontrol of Soft Rot Caused by Pectobacterium odoriferum with Bacteriophage phiPccP-1 in Kimchi Cabbage

Abstract

Pectobacterium odoriferum has recently emerged as a widely infective and destructive pathogen causing soft-rot disease in various vegetables. Bacteriophage phiPccP-1 isolated from Pyeongchang, South Korea, showed lytic activity against P. odoriferum Pco14 and two other Pectobacterium species. The transmission electron microscopy and genome phylograms revealed that phiPccP-1 belongs to the Unyawovirus genus, Studiervirinae subfamily of the Autographivirinae family. Genome comparison showed that its 40,487 bp double-stranded DNA genome shares significant similarity with Pectobacterium phage DU_PP_II with the identity reaching 98% of the genome. The phiPccP-1 application significantly inhibited the development of soft-rot disease in the mature leaves of the harvested Kimchi cabbage up to 48 h after Pco14 inoculation compared to the untreated leaves, suggesting that phiPccP-1 can protect Kimchi cabbage from soft-rot disease after harvest. Remarkably, bioassays with phiPccP-1 in Kimchi cabbage seedlings grown in the growth chamber successfully demonstrated its prophylactic and therapeutic potential in the control of bacterial soft-rot disease in Kimchi cabbage. These results indicate that bacteriophage phiPccP-1 can be used as a potential biological agent for controlling soft rot disease in Kimchi cabbage.

Karakterisasi Bakteri Penyebab Busuk Lunak pada Umbi Porang (Amorphophallus muelleri) Menggunakan Primer PCR Spesifik

“…Penyakit busuk lunak yang menyerang pada masa penyimpanan dapat menurunkan kualitas chip porang yang dijadikan sebagai produk ekspor ke beberapa negara, seperti Cina, Taiwan, dan Jepang. Busuk lunak merupakan salah satu penyakit perusak tanaman yang disebabkan oleh mikroorganisme patogen, salah satunya adalah infeksi bakteri (Lee et al 2021). Mikroorganisme patogen yang umum menyerang umbi adalah Erwinia carotovora dan P. carotovora pada A. konjac (Wu et al 2011), Dickeya dadantii pada A. rivier (Czajkowski et al 2011), dan P. stutzeri pada A. muelleri (Aini et al 2020).…”

Tailed Lytic Bacteriophages of Soft Rot Pectobacteriaceae

“…SRP Autographiviridae phages are promising as biocontrol agents. There were indications that treatment with both one phage and phage cocktails decreased tissue maceration and lesions because of soft rot disease, with an efficacy up to 98% [ 140 , 141 , 142 , 143 , 144 , 145 ]. Some phages (Jarilo, PP47, Q19, etc.)…”

Special Issue: Bacteriophage Treatment as an Alternative Technology to Inactivate Pathogenic Bacteria: A Generalized Worldwide Growing Acceptance

“…This Special Issue highlights recent advances in some of these areas. It includes fourteen papers focused on the use of phage therapy in a clinical setting, namely, to fight infections caused by multidrug-resistant bacteria [10][11][12] and by bacteria capable of forming biofilms [13], and in non-clinical fields, namely in the agro-food sector [14][15][16][17], including also the inactivation of bacteria forming biofilms [18,19]. Two of the papers [11,17] focus on the development of phage-resistant mutants after phage therapy, which is an important aspect for both clinical and non-clinical applications.…”

“…In agriculture studies, phage therapy was tested against Pectobacterium odoriferum, a bacterium that has recently emerged as a widely infective and destructive pathogen causing soft-rot disease in various vegetables, such as in Kimchi cabbage [16]. A new isolated bacteriophage showed lytic activity against P. odoriferum and two other Pectobacterium species.…”

“…A new isolated bacteriophage showed lytic activity against P. odoriferum and two other Pectobacterium species. The phage significantly inhibited the development of soft-rot disease in the mature leaves of harvested Kimchi cabbage up to 48 h after inoculation compared to the untreated leaves, suggesting that phages can protect Kimchi cabbage from soft-rot disease after harvest, thereby presenting prophylactic and therapeutic potential in the control of bacterial soft-rot disease [16]. Pinheiro et al [17] also described a phage application in the agriculture field.…”