Biocontrol of strawberry anthracnose disease caused by Colletotrichum nymphaeae using Bacillus atrophaeus strain DM6120 with multiple mechanisms

Alijani, Zahra; Amini, Jahanshir; Ashengroph, Morahem; Bahramnejad, Bahman; Mozafari, Ali Akbar

doi:10.1007/s40858-021-00477-7

Cited by 21 publications

(8 citation statements)

References 59 publications

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“…Therefore, in this study, we preliminarily found that the B. subtilis strain BS-1 had a significant inhibitory effect on kiwifruit soft rot, and the interaction between BS-1 and the pathogen B. dothidea was further investigated to assess its potential biotic mechanisms against kiwifruit soft rot. Our results were similar to those of B. subtilis in primary postharvest diseases of ecological fruits such as strawberry, grapes, tomato, and cucumber, where treatment with the fermentation solution (CFS) of B. subtilis inhibited the expansion of the pathogen in vitro and weakened the pathogenicity of pathogens on the host in a dose-dependent manner [ 35 , 36 , 37 , 38 ]. These indicate that B. subtilis has a broad-spectrum inhibitory effect and is effective against kiwifruit soft rot.…”

Section: Discussionsupporting

confidence: 77%

Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen

Fan

Liu

et al. 2023

JoF

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Biological control of postharvest diseases has been proven to be an effective alternative to chemical control. As an environmentally friendly biocontrol agent, Bacillus subtilis has been widely applied. This study explores its application in kiwifruit soft rot and reveals the corresponding mechanisms. Treatment with cell-free supernatant (CFS) of Bacillus subtilis BS-1 significantly inhibits the mycelial growth of the pathogen Botryosphaeria dothidea and attenuates the pathogenicity on kiwifruit in a concentration-dependent manner. In particular, mycelial growth diameter was only 21% of the control after 3 days of treatment with 5% CFS. CFS caused swelling and breakage of the hyphae of B. dothidea observed by scanning electron microscopy, resulting in the leakage of nucleic acid and soluble protein and the loss of ergosterol content. Further analysis demonstrated that CFS significantly induces the expression of Nox genes associated with reactive oxygen species (ROS) production by 1.9–2.7-fold, leading to a considerable accumulation of ROS in cells and causing mycelial cell death. Our findings demonstrate that the biocontrol effect of B. subtilis BS-1 CFS on B. dothidea is realized by inducing oxidative damage to the mycelia cell.

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Section: Discussionsupporting

confidence: 77%

Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen

Fan

Liu

et al. 2023

JoF

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“…Bacillus natto NT-6 produced considerably higher levels of iturin and surfactin in PDB, compared to Luria-Bertani (LB) and NB media [23]. PDB was also used to grow Bacillus strains for the control of the causative agents of various plant diseases, including mulberry fruit sclerotiniose, green mold, and strawberry anthracnose [24][25][26]. A major distinction between NB and PDB compositions was the absence and presence of sugar in the media, respectively.…”

Section: Resultsmentioning

confidence: 99%

Biocontrol Potential, Genome and Nonribosomal Peptide Synthetase Gene Expression of Bacillus velezensis 2211

Niemhom¹,

Kittiwongwattana²

2022

Curr. Appl. Sci. Technol.

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Members of the genus Bacillus produced a diverse group of antimicrobial compounds. Here, we presented the antifungal activity and genome sequence analysis of Bacillus sp. 2211, a potential plant-growth-promoting bacterium. Bacterial supernatants from strain 2211 cultures in nutrient broth (NB) and potato dextrose broth (PDB) suppressed the mycelial growth of Pyricularia oryzae, Colletotrichum aenigma, Colletotrichum fructicola and Fusarium oxysporum. The supernatants were also able to suppress spore germination of these fungi, except for F. oxysporum. However, the supernatant from PDB displayed a significantly higher inhibition activity than NB. Additionally, the supernatant from PDB significantly reduced the disease severity caused by P. oryzae on rice seedlings. The genome of strain 2211 was sequenced. The highest digital DNA-DNA hybridization (80.1%) and average nucleotide identity (97.57%) levels indicated that strain 2211 was a member of the species Bacillus velezensis. The phylogenomic analysis showed that it clustered with B. velezensis NRRL B-41580T, B. velezensis KACC 13105 and B. velezensis subsp. plantarum FZB42T. The gene expression analysis showed the up-regulation of nonribosomal peptide synthetase (NRPS) genes bmyA, fenB and dhbE in PDB, compared to NB. This work demonstrated that the culture media affected the antagonistic activity of strain 2211 possibly through the modification of NRPS biosynthesis genes.

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“…Other laboratory analyses, such as microscopy, molecular, biochemical, and microbiological methods, have been applied for the detection of crop diseases; however, these techniques have disadvantages, as the sampling process is destructive and offers limited diagnostic points, and it is not field-scalable or may not represent accurate field variability [47,49]. Therefore, precise, high-throughput, non-invasive, and field-scalable approaches are required [47,50]. As an alternative in recent years, non-destructive methods such as spectral vegetation indexing, multispectral imaging (MSI), or hyperspectral imaging (HSI) based on ground, aerial, and satellite platforms have emerged that are capable of crop disease diagnostics at high accuracy and on high spatial scales (from leaf to plant to field).…”

Section: Detection: Morphological Molecular and Remote Sensingmentioning

confidence: 99%

New Insights in the Detection and Management of Anthracnose Diseases in Strawberries

Aljawasim,

Samtani,

Rahman

2023

Plants

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Anthracnose diseases, caused by Colletotrichum spp., are considered to be among the most destructive diseases that have a significant impact on the global production of strawberries. These diseases alone can cause up to 70% yield loss in North America. Colletotrichum spp. causes several disease symptoms on strawberry plants, including root, fruit, and crown rot, lesions on petioles and runners, and irregular black spots on the leaf. In many cases, a lower level of infection on foliage remains non-symptomatic (quiescent), posing a challenge to growers as these plants can be a significant source of inoculum for the fruiting field. Reliable detection methods for quiescent infection should play an important role in preventing infected plants’ entry into the production system or guiding growers to take appropriate preventative measures to control the disease. This review aims to examine both conventional and emerging approaches for detecting anthracnose disease in the early stages of the disease cycle, with a focus on newly emerging techniques such as remote sensing, especially using unmanned aerial vehicles (UAV) equipped with multispectral sensors. Further, we focused on the acutatum species complex, including the latest taxonomy, the complex life cycle, and the epidemiology of the disease. Additionally, we highlighted the extensive spectrum of management techniques against anthracnose diseases on strawberries and their challenges, with a special focus on new emerging sustainable management techniques that can be utilized in organic strawberry systems.

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Biocontrol of strawberry anthracnose disease caused by Colletotrichum nymphaeae using Bacillus atrophaeus strain DM6120 with multiple mechanisms

Cited by 21 publications

References 59 publications

Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen

Cell-Free Supernatant of Bacillus subtilis Reduces Kiwifruit Rot Caused by Botryosphaeria dothidea through Inducing Oxidative Stress in the Pathogen

Biocontrol Potential, Genome and Nonribosomal Peptide Synthetase Gene Expression of Bacillus velezensis 2211

New Insights in the Detection and Management of Anthracnose Diseases in Strawberries

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