Biocontrol potential and polyphasic characterization of novel native Trichoderma strains against Macrophomina phaseolina isolated from sorghum and common bean

Abstract

Native strains of Trichoderma isolated from sorghum and common bean crop soils were investigated to assess their biocontrol potential over the phytopathogenic fungus Macrophomina phaseolina, isolated from diseased plants. The Trichoderma strains were characterized with a polyphasic approach, which combined the analysis of their morphological characteristics, enzymatic activity, macro- and microculture test results, rDNA restriction patterns (AFLP), ITS1-5.8S-ITS2 rDNA sequences, and protein profiles. The integration of these data sets can be used to select new isolates as biological control agents against native fungal phytopathogens. In general, we observed a positive correlation between the secretion of beta-1,3-glucanase and N-acetylhexosaminidase, and the biocontrol capacities of all the Trichoderma isolates. Strains with the best hyperparasitic behavior against M. phaseolina isolated from diseased bean and sorghum were Trichoderma sp. (TCBG-2) and Trichoderma koningiopsis (TCBG-8), respectively.

Antagonistic and Biocontrol Potential of Trichoderma asperellum ZJSX5003 Against the Maize Stalk Rot Pathogen Fusarium graminearum

“…The challenge remains to determine which secondary metabolites would be most effective in mitigating the effects of Fusarium and how they can be applied to corn seedlings for a sustainable effect. Differential antagonism activity has been observed for Trichoderma isolates belonging to the same species, as shown by a semi-specificity in the interaction of Trichoderma with spores of Botrytis cinerea and M. phaseolina [32,33]. Testing for the strain of Trichoderma with the highest antagonistic activity is important for developing biocontrol agents.…”

Genetic characterization of <i>Burkholderia cepacia</i> strain from Northeast India: A potential bio-control agent

“…In the present study, several indigenous bacterial strains were isolated from Imphal East and West districts of French bean growing area and characterized by phenotypic and genotypic approach and investigated for their ability to inhibit the growth of Fusarium oxysporum and Rhizoctonia solani. To the best of our knowledge, indigenous native isolates performs best in terms of its specificity in a particular environment where it survive [31]. The use of indigenous bacterial isolates such as Pseudomonas and Burkholderia species has not been reported so far from NE India for the control of soil borne diseases in french beans.…”

Impact of Trichoderma spp. on soybean seed germination and potential antagonistic effect on Sclerotinia sclerotiorum

“…Nowadays, numerous commercial biofungicides, such as Bio-Fungus (Belgium), Trichodex (Israel), Trieco (India), Supresivit (U.S., Europe), Promot (California), Binab T (U.K., Sweden), Root Shield (USA, Europe), etc., are based on Trichoderma viride, T. harzianum and T. koningii strains that are efficient biocontrol agents of several phytopathogenic fungi (https://attra.ncat.org/attra-pub/summaries/summary.php?pub=146). So far, Trichoderma species have been mainly registered as effective antagonists of soilborne pathogens, such as Sclerotinia sclerotiorum (Mansour et al, 2008;Matroudi et al, 2009;Ibarra-Mediná et al, 2010), S. minor (Jones and Stewart, 1997;Ibarra-Mediná et al, 2010), Macrophomina phaseolina (Ilyas et al, 1985;Larralde-Corona et al, 2008), Rhizoctonia solani (Maisura and Patel, 2009;da Silva et al, 2012), Fusarium spp. (Sempere and Santamarina, 2009;Zang and Wang, 2012), Pythium spp.…”