Biocontrol potential of cyanobacterial metabolites against damping off disease caused by<i>Pythium aphanidermatum</i>in solanaceous vegetables

Manjunath, M.; Prasanna, Radha; Nain, Lata; Dureja, Prem; Singh, Rajendra; Kumar, Arun; Jaggi, Sidharth; Kaushik, B. D.

doi:10.1080/03235400802075815

Cited by 86 publications

(27 citation statements)

References 12 publications

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“…The global biocontrol regulatory genes in S. marcescens and the diverse mechanisms exhibited by them may be significantly contributing to the bioactivity of biofilms in which they are associated. Among cyanobacteria, Nostoc muscorum and several Anabaena and Calothrix strains exhibit fungicidal activity against species of Pythium, Fusarium, and Rhizoctonia (Moon et al 1992;Prasanna 2008;Radhakrishnan et al 2009;Manjunath et al 2010); however, the strain being used by us did not exhibit any fungicidal activity.…”

Section: Discussionmentioning

confidence: 84%

Development of cyanobacterium-based biofilms and their in vitro evaluation for agriculturally useful traits

et al. 2011

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The ability of cyanobacteria to be useful as matrices for agriculturally important bacteria was evaluated. Biofilms were generated with the selected strain Anabaena torulosa after co-culturing with Azotobacter chroococcum, Pseudomonas striata, Serratia marcescens, and Mesorhizobium ciceri. The biochemical attributes were compared with individual bacterial and cyanobacterial cultures. The biofilms were characterized in terms of proteins, chlorophyll, IAA production, acetylene-reducing activity, phosphate solubilization, and antagonism towards selected phytopathogenic fungi. An enhancement in the population counts was recorded in A. torulosa-S. marcescens and A. torulosa-P. striata biofilms. The A. torulosa-A. chroococcum and A. torulosa-M. ciceri biofilms were also able to utilize new saccharides as compared to the individual cultures. Such novel biofilms with agriculturally useful traits can provide additional advantages including the broader spectrum of activity and the presence or formation of biologically active compounds; they also suggest the way to effective inoculants for sustainable and environment friendly agriculture.

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Section: Discussionmentioning

confidence: 84%

Development of cyanobacterium-based biofilms and their in vitro evaluation for agriculturally useful traits

et al. 2011

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“…However, most of the research has been focused on pharmaceutical applications of cyanobacteria-derived products and the potential use of cyanobacteria in agriculture is often reported as a secondary goal (except in a few cases), with research in this field being limited to in vitro screening (Mahakhant et al 1998). Some antifungal molecules derived from cyanobacteria have also been patented/utilized for agricultural use, but only limited information is available (Hirsch et al 1990;Manjunath et al 2009). …”

Section: Resultsmentioning

confidence: 98%

Bioprospecting for genes involved in the production of chitosanases and microcystin-like compounds in Anabaena strains

Prasanna

Gupta

Natarajan

et al. 2009

World J Microbiol Biotechnol

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Bioinformatic tools guided PCR amplification assays were employed for analyzing two Anabaena strains A. laxa and A. iyengarii which exhibited chitosanase activity, allelopathic and fungicidal activity. Sequencing of a 297 bp fragment obtained by amplification with primers directed towards mcy A gene (involved in the production of microcystins), revealed significant similarity with the condensation domain, while amplification with specific primers towards N-methyltransferase (NMT) domain showed 59% similarity with a homologous domain in a toxic strain of Microcystis aeruginosa. An amplified product of 172 bp obtained using specific primers derived from the coding region of chitinase (chi IS) gene in Streptomyces sp., showed 100% similarity with hydrogenbyrinic acid a, cdiamide cobaltochelatase gene in Anabaena, and significant similarity with chi IS gene of Streptomyces sp. under less stringent conditions. The 663 bp sequence obtained by employing specific primers for chitosanase (choA) derived from Mitsuaria chitosanitabida 3001 strain, showed 100% similarity with glycoside hydrolase family three domain like protein(s). This study is a first time report on the presence of homologues of chitosanase in cyanobacteria which can play a role in allelopathic activity exhibited by these oxygenic photosynthetic prokaryotes.

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“…Such products are available commercially under various trade names. Towards this endeavor, recently, we showed the presence of hydrolytic activity in these organisms and demonstrated their role in biocidal activity against phytopathogenic fungi Manjunath et al 2010). From our previous investigations, we selected A. laxa RPAN8 for indepth analyses of biochemical and molecular aspects of fungicidal activity.…”

Section: Discussionmentioning

confidence: 98%

Identification and characterization of endoglucanases for fungicidal activity in Anabaena laxa (Cyanobacteria)

et al. 2010

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A cyanobacterial strain (Anabaena laxa RPAN8) exhibiting fungicidal activity and β-1,3 and 1,4 endoglucanase activities was selected for identifying the gene(s) involved. Functional analyses of the genomic library revealed that four clones (8, 64, 116, and 248) of RPAN8 exhibited fungicidal activity and induced structural deformities in the cell wall of the growing mycelia of Pythium aphanidermatum. Higher expression of fungicidal and β-1,4 endoglucanase activities, along with low expression of β-1,3 endoglucanase activity, was recorded in two E. coli clones (8 and 64). Clones 8 and 64 exhibited identical sequences while clones 116 and 248 were also similar. Bioinformatic analyses were undertaken only for the two nonidentical clones 8 and 116 which showed open reading frames (ORFs) of 348 (end 1) and 656 amino acid residues (end 2), respectively. The amino acid sequence analyses revealed that the end 1 encoding endoglucanases belonged to peptidase M20 family while end 2 showed significant similarities with several known genes. The putative promoters and ribosomal binding sites were identified and amino acid exchanges were observed in both end 1 and 2. The presence of signal peptides of 24 and 20 amino acid residues respectively revealed the secretory nature of these proteins.

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Biocontrol potential of cyanobacterial metabolites against damping off disease caused byPythium aphanidermatumin solanaceous vegetables

Cited by 86 publications

References 12 publications

Development of cyanobacterium-based biofilms and their in vitro evaluation for agriculturally useful traits

Development of cyanobacterium-based biofilms and their in vitro evaluation for agriculturally useful traits

Bioprospecting for genes involved in the production of chitosanases and microcystin-like compounds in Anabaena strains

Identification and characterization of endoglucanases for fungicidal activity in Anabaena laxa (Cyanobacteria)

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