Biocontrol Potential of Streptomyces griseus H7602 Against Root Rot Disease (Phytophthora capsici) in Pepper

Abstract

The root rot of pepper (Capsicum annuum L.) caused by Phytophthora capsici is one of the most important diseases affecting this crop worldwide. This work presents the evaluation of the capacity of Streptomyces griseus H7602 to protect pepper plants against Phytophthora capsici and establishes its role as a biocontrol agent. In this study, we isolated an actinomycete strain H7602 from rhizosphere soil, identified it as Streptomyces griseus by 16S rRNA analysis and demonstrated its antifungal activity against various plant pathogens including P. capsici. H7602 produced lytic emzymes such as chitinase, β-1,3-glucanase, lipase and protease. In addition, crude extract from H7602 also exhibited destructive activity toward P. capsici hyphae. In the pot trial, results showed the protective effect of H7602 against pepper from P. capsici. Application of H7602 culture suspension reduced 47.35% of root mortality and enhanced growth of pepper plants for 56.37% in fresh root and 17.56% g in fresh shoot as compared to control, resulting in greater protection to pepper plants against P. capsici infestation. Additionally, the enzymatic activities, chitinase and β-1,3-glucanase, were higher in rhizosphere soil and roots of pepper plants treated with H7602 than other treated plants. Therefore, our results indicated a clear potential of S. griseus H7602 to be used for biocontrol of root rot disease caused by P. capsici in pepper.

Isolation and Identification of Bacillus amyloliquefaciens IBFCBF‐1 with Potential for Biological Control of Phytophthora Blight and Growth Promotion of Pepper

“…(Yu et al 2011;Yang et al 2015), Trichoderma sp. (Joo 2005;Ko et al 2010;Nguyen et al 2012), Burkholderia cepacia (Ezziyyani et al 2004), Pseudomonas spp. (Joo 2005;Ko et al 2010;Nguyen et al 2012), Burkholderia cepacia (Ezziyyani et al 2004), Pseudomonas spp.…”

Efecto de biocontrol e inhibición germinativa de Bacillus spp. sobre zoosporas de Phytophthora capsici

“…Aunque algunos fungicidas específicos han logrado reducir la severidad de la enfermedad en forma rápida y efectiva, su uso indiscriminado ha ocasionado la aparición de cepas resistentes y contaminación al ambiente (Qi et al, 2012). Por lo anteriormente señalado, en la actualidad para el manejo de Phytophthora se han considerado nuevas alternativas, basadas en el control biológico, estrategia importante en el manejo de fitopatógenos que habitan en el suelo, ya que reducen la aplicación de agroquímicos (Nguyen et al, 2012;Rios-Velasco et al, 2016).…”

“…Los mecanismos de control incluyen la producción de has led to the emergence of resistant isolates and environmental pollution (Qi et al, 2012). For this reason, new alternatives based on biological control are being considered for managing Phytophthora, an important strategy in soil-borne phytopathogen management, since they reduce the application of agrochemicals (Nguyen et al, 2012;Rios-Velasco et al, 2016). Different microorganisms have been reported to suppress P. capsici growth, including Streptomyces spp.…”

“…Different microorganisms have been reported to suppress P. capsici growth, including Streptomyces spp. (Ko et al, 2010;Nguyen et al, 2012), Paenibacillus spp. (Naing et al, 2014), Trichoderma sp.…”

Biological Control of Oomycetous Plant Pathogens: A Review

“…Various antibiotics and lytic enzymes produced by microorganisms revealed antagonism against oomycetes (Dunne et al 1997, Timmusk et al 2009, Muthukumar et al 2011. Recently, Streptomyces producing chitinase, ß-1, 3-glucanase, lipase and protease showed direct lysis of Phytophthora capsici hyphae (Nguyen et al 2012). In addition, Pseudomonas ûuorescens and Serratia plymuthi showed the antagonisms to Pythium aphanidermatum and Phytophthora capsici (Muthukumar et al 2011, Shen et al 2002.…”