Bioconversion of Glutamic acid to γ-Aminobutyric acid (GABA) in Brown Rice Grains Induced by High Pressure Treatment

Shigematsu, Toru; Murakami, Mina; Nakajima, Kanako; Uno, Yoshiyuki; Sakano, A; Narahara, Yuka; Hayashi, Mayumi; Ueno, S.; Fujii, Tomoyuki

doi:10.11301/jsfe.11.189

Cited by 21 publications

(17 citation statements)

References 37 publications

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“…These values are lower than those found for brown rice, in which the initial GABA production rate reached 3.5 mmol/g/d with an initial Glu concentration of 23 mmol/g (0.05 g/mL of Glu soaking solution). 25) The effect of the HHP treatment on GABA production in both soybean and brown rice is similar, even though the internal structures of these materials are significantly different, especially regarding the distribution of enzymes.…”

Section: Gad Activity Testmentioning

confidence: 94%

“…Although the GC-FID analysis in this study could not distinguish between GABA and serine (Ser), we could show that the major component of the GABA and Ser peak was attributable to GABA by using electro-spray ionization mass spectrometry (CE-ESI-MS/MS). 25) Using free amino acid samples that had been diluted to within the appropriate range, the value for GABA/Ser was determined according to the result from the free amino acid analysis kit, using an absolute standard curve that was constructed with a GABA concentration range up to 4 mg/mL. The Glu concentration measured at day 0 was determined as the initial value and was applied to the kinetic analysis.…”

Section: Methodsmentioning

confidence: 99%

“…The GABA production rates in the HHP-treated and untreated brown rice in our previous study 25) were also fitted by the substrate inhibition model. The value of K m for the HHP-treated brown rice (440 mmol/g) was significantly higher than that for the untreated brown rice (69 mmol/g).…”

Section: Gad Activity Testmentioning

confidence: 99%

“…25) The initial GABA production rate in the untreated soybeans was not affected by an The initial GABA production rate was calculated from the slope of the GABA concentration time-course characteristics during 2 d of storage. The substrate inhibition kinetic model from Equation (1) was applied for each sample.…”

Section: Gad Activity Testmentioning

confidence: 99%

“…Although the HHP-induced changes in compound levels result from random enzymatic reactions, controlling these enzymatic reactions to induce a large accumulation of target compounds has hardly been reported for HHP-treated foodstuffs. 25) The generation of free amino acids in water-soaked and HHP-treated soybean seeds has been previously investigated, 26) and when compared with untreated Glu-soaked soybeans, the HHP-treated Glu-soaked (0.05 g/mL of Glu) soybeans had a higher GABA concentration, suggesting that y To whom correspondence should be addressed. Fax: +81-22-717-8946; E-mail: shigeakiu@hotmail.com both proteolysis and specific Glu metabolism were accelerated by HHP.…”

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confidence: 99%

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Enzymatic Production of γ-Aminobutyric Acid in Soybeans Using High Hydrostatic Pressure and Precursor Feeding

Ueno

Katayama

Watanabe

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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The effects were investigated of the glutamic acid (Glu) substrate concentration on the generation and kinetics of γ-aminobutyric acid (GABA) in soybeans treated under high hydrostatic pressure (HHP; 200 MPa for 10 min at 25 °C). The conversion of Glu to GABA decreased with increasing initial Glu concentration in the soybeans. The crude glutamate decarboxylase (GAD) obtained from the HHP-treated soybeans showed substrate inhibition. The GABA production rate in the HHP-treated soybeans fitted the following substrate inhibition kinetic equation: v0=(VmaxS0)/(Km+S0+(S0)2/Ki). The Km value for the HHP-treated soybeans was significantly higher than that of the untreated soybeans. The Km values in this study show the affinity between Glu and GAD, and indicate that the HHP-treated soybeans had lower affinity between Glu and GAD than the untreated soybeans. GAD extracted from the HHP-treated soybeans showed a similar value to that in the HHP-treated soybeans. The intact biochemical system was so damaged in the HHP-treated soybeans that it showed substrate inhibition kinetics similar to that of the extracted GAD. The combination of HHP and precursor feeding proved to be a novel tool that can be used to increase the concentration of a target component.

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Section: Gad Activity Testmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Gad Activity Testmentioning

confidence: 99%

Section: Gad Activity Testmentioning

confidence: 99%

mentioning

confidence: 99%

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Enzymatic Production of γ-Aminobutyric Acid in Soybeans Using High Hydrostatic Pressure and Precursor Feeding

Ueno

Katayama

Watanabe

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Influence of high‐pressure processing on the generation of γ‐aminobutyric acid and microbiological safety in coffee beans

et al. 2018

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Investigation of growth behavior for rice sprout during heterotrophic cultivation and the potential for high‐density cultivation

Ishikawa,

Kotani,

Tamate

et al. 2024

Urban Agric & Regnl Food Sys

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The efficient production of useful substances in plant factories is very attractive to secure the nutrition for human health in the future. The aim of this study was to investigate the growth behavior of rice sprouts to establish a high‐density production system for plant factories. To clarify the material balance during the growth of sprout of brown rice, a single individual of rice was cultivated in vitro. The weight of the sample and the oxygen and carbon dioxide concentrations in the test tube were measured, with bioactivity indices being calculated. The contribution of growth respiration was confirmed based on the growth, oxygen uptake, and carbon dioxide evolution rates. The specific growth rates and oxygen concentrations all increased during the 60‐ to 80‐h period. The decrease in the specific oxygen uptake rate and carbon dioxide evaluation rate generally corresponded to the 90‐ to 160‐h period. These results suggest that the physiological state of rice sprouts can be evaluated using bioactivity indices obtained from individual cultivation systems. A trial application for the high‐density heterotrophic cultivation of rice sprouts was performed using the developed container. The growth productivity of rice sprouts cultivated in containers was similar to that of individual test tubes. These results showed the new value of crop cultivation by the plant factory.

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Bioconversion of Glutamic acid to γ-Aminobutyric acid (GABA) in Brown Rice Grains Induced by High Pressure Treatment

Cited by 21 publications

References 37 publications

Enzymatic Production of γ-Aminobutyric Acid in Soybeans Using High Hydrostatic Pressure and Precursor Feeding

Enzymatic Production of γ-Aminobutyric Acid in Soybeans Using High Hydrostatic Pressure and Precursor Feeding

Influence of high‐pressure processing on the generation of γ‐aminobutyric acid and microbiological safety in coffee beans

Investigation of growth behavior for rice sprout during heterotrophic cultivation and the potential for high‐density cultivation

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