Enzymatic Production of γ-Aminobutyric Acid in Soybeans Using High Hydrostatic Pressure and Precursor Feeding

Ueno, S.; Katayama, Takumi; Watanabe, Takae; Nakajima, Kanako; Hayashi, Mayumi; Shigematsu, Toru; Fujii, Tomoyuki

doi:10.1271/bbb.120740

Cited by 16 publications

(8 citation statements)

References 46 publications

(56 reference statements)

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“…The amount of glutathione (GSH) was determined as described (Ueno et al . ), and the concentration was calculated against commercially available standard of GSH. The glutamic acid decarboxylase (GAD) activity was determined according to Ueno et al .…”

Section: Methodsmentioning

confidence: 99%

“…The glutamic acid decarboxylase (GAD) activity was determined according to Ueno et al . (), with γ‐aminobutyric acid (GABA) as standard. One unit of GAD activity was defined as the production of 1 µmol GABA per hour.…”

Section: Methodsmentioning

confidence: 99%

“…The amount of glutathione (GSH) was determined as described (Ueno et al 2013), and the concentration was calculated against commercially available standard of GSH. The glutamic acid decarboxylase (GAD) activity was determined according to Ueno et al (2013), with γ-aminobutyric acid (GABA) as standard. One unit of GAD activity was defined as the production of 1 μmol GABA per hour.…”

Section: Glutathione Concentration and Glutamic Acid Decarboxylase Acmentioning

confidence: 99%

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A multilevel investigation to discover why Kandelia candel thrives in high salinity

Wang

Pan

et al. 2016

Plant Cell & Environment

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The halophilic mangrove species Kandelia candel is an excellent model for understanding why halophytes thrive in high salinity. Preliminary transcriptomic analyses revealed that genes involved in diverse functions, such as in phenylpropanoid and amino acid metabolisms, and those in DNA replication and damage repair were highly responsive to salt stress. Proteomic analyses revealed that the proteins involved in light reaction of photosynthesis, amino acid and carbohydrate metabolisms, secondary metabolite biosynthesis and posttranslational modification showed increased levels in response to salt stress. The metabolisms of phenylpropanoids and amino acids under salt stress were systematically examined based on the preliminary omics analyses. The activities of phenylpropanoid biosynthetic enzymes and the contents of phenols, flavonoids, anthocyanins and lignins were significantly increased under salt stress. In the free amino acid pool, glutamate was the most abundant. Together with γ-aminobutyric acid, glutamate levels further increased, while proline levels remained unchanged in response to salt stress. These findings point to the potential importance of phenylpropanoids and free amino acids in salt tolerance of K. candel that have been observed, but not systemically investigated at the levels of gene expression, enzyme activity and metabolite accumulation in glycophytes and non-tree halophytes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Glutathione Concentration and Glutamic Acid Decarboxylase Acmentioning

confidence: 99%

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A multilevel investigation to discover why Kandelia candel thrives in high salinity

Wang

Pan

et al. 2016

Plant Cell & Environment

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“…High-pressure-induced damages to cellular structure were also elucidated in a separate study on the relative drying rate (RDR) of Japanese radish samples before and after HHP pretreatment; the RDR was found to be similar to that after chloroform vapor treatment but lower than that in the case of heat treatment and freeze-thaw pretreatment [ 7 ]. HHP on legumes such as green bean [ 8 , 9 ] chickpea [ 10 ], cowpea [ 11 ], and soybean [ 12 , 13 ] had been reported from the viewpoints of structural, physical or biochemical changes. However, in another study, Eshtiaghi et al .…”

Section: Introductionmentioning

confidence: 99%

Effects of High Hydrostatic Pressure on Water Absorption of Adzuki Beans

Ueno

Shigematsu

Karo

et al. 2015

Foods

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The effect of high hydrostatic pressure (HHP) treatment on dried soybean, adzuki bean, and kintoki kidney bean, which are low-moisture-content cellular biological materials, was investigated from the viewpoint of water absorption. The samples were vacuum-packed with distilled water and pressurized at 200 MPa and 25 °C for 10 min. After the HHP treatment, time courses of the moisture contents of the samples were measured, and the dimensionless moisture contents were estimated. Water absorption in the case of soybean could be fitted well by a simple water diffusion model. High pressures were found to have negligible effects on water absorption into the cotyledon of soybean and kintoki kidney bean. A non-linear least square method based on the Weibull equation was applied for the adzuki beans, and the effective water diffusion coefficient was found to increase significantly from 8.6 × 10−13 to 6.7 × 10−10 m2/s after HHP treatment. Approximately 30% of the testa of the adzuki bean was damaged upon HHP treatment, which was comparable to the surface area of the testa in the partially peeled adzuki bean sample. Thus, HHP was confirmed to promote mass transfer to the cotyledon of legumes with a tight testa.

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“…It is speculated that the HPP of 600 Mpa treatment may cause severe damage to those enzymes that convert glutamate to related metabolites, so there is a higher amount of glutamate accumulation. Ueno et al [ 52 ] evaluated the effects of treatment of soybeans at 200 MPa for 10 min at 25 °C on L-glutamate concentration, GABA production, and their kinetics. Their results showed that the conversion of L-glutamate to GABA decreased with increasing initial L-glutamate concentration in soybeans.…”

Section: Resultsmentioning

confidence: 99%

Increasing γ-Aminobutyric Acid Content in Vegetable Soybeans via High-Pressure Processing and Efficacy of Their Antidepressant-Like Activity in Mice

Chang

Lai

et al. 2020

Foods

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This study applied high-pressure processing (HPP) technology to enrich the gamma aminobutyric acid (GABA) content in vegetable soybeans and evaluated its antidepressant efficacy on mice, with depression induced by the unpredictable chronic mild stress (UCMS) model. The optimal conditions for HPP, storage time, and storage temperature, as well as antidepressant-like effects of vegetable soybeans, were evaluated and discussed. HPP could effectively and significantly increase GABA content in soybean, with optimum conditions at 200 MPa. The GABA content in the whole vegetable soybean was 436.05 mg/100 g. In mice animal tests, the tail suspension test (TST) showed that the immobility time of the GABA group was significantly shorter than that of the control group. The total travel distance in the open field test (OFT) showed that depressed mice fed with the GABA feed exhibited exploratory behavior. The GABA group showed a significantly higher degree of sucrose preference than the control group. Both results indicate that the GABA feed could effectively alleviate depressive symptomatology. Regarding biochemical parameters, the fecal and serum corticosterone (CORT) levels in the control group increased to 104.86 pg/mg after the onset of depression. In contrast, the fecal CORT level in the GABA group was significantly reduced to 23.98 pg/mg and was comparable to that in the control group (33.38 pg/mg). Reduced serum CORT level in the GABA group suggests an improvement in depressive symptomatology. The serotonin concentration was maintained in the GABA group after the induction of depression, suggesting its preventive activity. The HPP GABA-enriched soybeans exerted modulatory effects on the behaviors of depressed mice and displayed a potential for commercialization.

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Enzymatic Production of γ-Aminobutyric Acid in Soybeans Using High Hydrostatic Pressure and Precursor Feeding

Cited by 16 publications

References 46 publications

A multilevel investigation to discover why Kandelia candel thrives in high salinity

A multilevel investigation to discover why Kandelia candel thrives in high salinity

Effects of High Hydrostatic Pressure on Water Absorption of Adzuki Beans

Increasing γ-Aminobutyric Acid Content in Vegetable Soybeans via High-Pressure Processing and Efficacy of Their Antidepressant-Like Activity in Mice

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