Bioconversion of industrial wastewater from palm oil processing to butanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Hipólito, Cirilo Nolasco; Crabbe, Edward; Badillo, Carmen Monterrubio; Zarrabal, Octavio Carvajal; Morales-Mora, M. A.; Flores, Gabriel Pineda; Cortazar, Manuel de A. Hernández; Ishizaki, Ayaaki

doi:10.1016/j.jclepro.2007.02.005

Cited by 67 publications

(20 citation statements)

References 17 publications

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“…46 Jesse et al 47 performed the fermentation of waste streams such as starch-based packing for peanuts and agricultural wastes as a source of fermentable carbohydrates using C. beijerinckii BA101 and produced 21.7 g.l -1 and 14.8 g.l -1 ABE, respectively. The use of enzyme hydrolysate of palm oil mill effluent has been reported as a growth medium substitute, a fermentation substrate, and as a source of nitrogen and micronutrients for the production of ABE by Hipolito et al 48 The agricultural wastes such as wheat bran, rice bran, maize stalk, barley straw etc. were studied by various researchers as sugar sources.…”

Section: Fermentation With Biomass Hydrolysatementioning

confidence: 99%

Biobutanol: the outlook of an academic and industrialist

et al. 2013

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The gradual shift of transportation fuels from oil based fuels to the alternative fuel resources and worldwide demand for energy has been the impetus for research to produce alcohol biofuels from renewable resources. Current bioethanol and biodiesel can, however, not cover an increasing demand for biofuels. Hence, there is an extensive need for advanced biofuels with superior fuel properties. The present review is focused on the developments of biobutanol, which is regarded to be superior to bioethanol in terms of energy density and hygroscopicity. Although acetone-butanolethanol (ABE) fermentation is one of the oldest large-scale fermentation processes, butanol yield by anaerobic fermentation remains sub-optimal. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, low product yield, product toxicity to production strain, multiple end-products and downstream processing of alcohol mixtures.Metabolic engineering provides a means for fermentation improvements. Different strategies are employed in the metabolic engineering of Clostridia that aim to enhance the solvent production, improve selectivity for butanol production, and increase the tolerance of Clostridia to solvents. The introduction and expression of a non-clostridial butanol producing pathway in E. coli is most promising strategy for butanol biosynthesis. Several rigorous kinetic and physiological models for fermentation have been formulated, which form useful tool for optimization of the process. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to improve production economics. With the use of new strains, inexpensive substrates, and superior reactor designs, economic ABE fermentation may further attract an attention of researchers all over the world. The present review is attempting to provide an overall outlook on discoveries and strategies that are being developed for commercial n-butanol production.

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Section: Fermentation With Biomass Hydrolysatementioning

confidence: 99%

Biobutanol: the outlook of an academic and industrialist

et al. 2013

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“…A similar phenomenon was observed when crude palm oil (CPO) was added to palm oil mill effluent medium to investigate the effect of CPO on ABE fermentation using C. saccharoperbutylacetonicum N1-4 (data not shown). An earlier study reported that the effect of oil on ABE fermentation by C. saccharoperbutylacetonicum N1-4 can be attributed to interference with sugar uptake and solvent production during fermentation [30]. Extraction of the oil from RB can facilitate greater solvent production at a lesser cost and the extracted oil can be used in other applications, such as biodiesel production, food or medical uses.…”

Section: Abe Fermentation Using Sarb-and Sadrb-based Mediummentioning

confidence: 99%

Biobutanol production from rice bran and de-oiled rice bran by Clostridium saccharoperbutylacetonicum N1-4

Al-Shorgani

Yusoff

2011

Bioprocess Biosyst Eng

100

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Rice bran (RB) and de-oiled rice bran (DRB) have been treated and used as the carbon source in acetone-butanol-ethanol (ABE) production using Clostridium saccharoperbutylacetonicum N1-4. The results showed that pretreated DRB produced more ABE than pretreated RB. Dilute sulfuric acid was the most suitable treatment method among the various pretreatment methods that were applied. The highest ABE obtained was 12.13 g/L, including 7.72 g/L of biobutanol, from sulfuric acid. The enzymatic hydrolysate of DRB (ESADRB), when treated with XAD-4 resin, resulted in an ABE productivity and yield of 0.1 g/L h and 0.44 g/g, respectively. The results also showed that the choice of pretreatment method for RB and DRB is an important factor in butanol production.

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“…For a long-term stock culture, C. saccharoperbutylacetonicum N1-4 (ATCC 13564) was previously kept as spores in sterilized sand, while for short-term storage, it was maintained in 15% PG (potato glucose) medium containing substances mentioned in previous reports (Hipolito et al 2008;Tashiro et al 2007). For refreshing the stock culture, per 1 mL of this stock was transferred into 9 mL of fresh PG medium, heatshocked in boiling water for 1 min, cooled in iced water for several min and anaerobically incubated at 30 °C for 28 h without agitation or pH control.…”

Section: Methodsmentioning

confidence: 99%

Production of Acetone, Butanol and Ethanol as Bioenergy Source Materials by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using Different Substrates

Ambarsari¹,

Sonomoto

2013

Microbiol Indones

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The cost of raw materials has been known to be the major limitation on the economic feasibility of acetone-butanol-ethanol (ABE) production. About 60-70% of the total ABE production cost is the cost for substrates (Madihah et al. 2001). Therefore, many researches have been conducted to find the most economical substrates for ABE production, for examples those who used simple sugars such as glucose, lactose, galactose, and xylose (Shinto et al.2008;Keis et al. 2001;Bahl et al. 1986), or moreIn the effort of developing bioenergy source materials, a laboratorium study was performed to investigate the effects of substrate types and concentrations on the production of acetone-butanol-ethanol (ABE) from various substrates by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in defined TYA (Tryptone-Yeast extract-Acetate) media. The results demonstrated that the ABE ratios produced by the strain N1-4 were greatly influenced by the types of substrate and the concentrations being used. It was also shown that fermentation of disaccharides (cellobiose and dextrin) by the strain N1-4 could give relatively as high butanol and ABE yields as glucose fermentation after 24 h fermentation time. The strain N1-4 was also found to be able to ferment xylans from birchwood sources producing a relatively high ABE concentration, especially at a prolonged incubation time (after 48 h incubation period). A subsequent experiment using several different concentrations of glucose or cellobiose revealed that the higher the concentration of the substrate being used, then the higher the total ABE concentration or productivity could be obtained but not necessarily in the same increasing ratio. To the best of our knowledge, there was no other previous study about the effect of substrate type and concentration on the ABE ratio by C. saccharoperbutylacetonicum N1-4 (ATCC 13564) using various substrates in defined TYA media, specifically by using xylans and dextrin substrates.Key words: ABE ratio, acetone-butanol-ethanol fermentation, biomass substrate effect, Clostridium saccharoperbutylacetonicum N1-4Dalam rangka mendapatkan bahan baku bioenergi, sebuah studi laboratorium dilakukan untuk mengetahui pengaruh dari jenis dan konsentrasi substrat biomasa terhadap proses fermentasi langsung yang menghasilkan aseton, butanol, dan etanol oleh bakteri Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) dalam media TYA (Tryptone-Yeast extract-Acetate) yang terdefinisi. Hasil penelitian menunjukkan bahwa rasio dari aceton:butanol:etanol (rasio ABE) ternyata sangat dipengaruhi oleh jenis substrat biomass dan konsentrasi yang dipergunakan dalam proses fermentasinya. Data menunjukkan bahwa dengan menggunakan substrat disakarida (yang diwakili oleh dextrin dan cellobiose), strain N1-4 mampu menghasilkan ABE dengan yield yang relatif sama tinggi dengan menggunakan glukosa setelah masa fermentasi 24 jam. Strain N1-4 ternyata juga mampu memproduksi ABE dengan konsentrasi yang cukup tinggi dengan menggunakan substrat xylan yang diekstraksi dari kayu, wa...

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Bioconversion of industrial wastewater from palm oil processing to butanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

Cited by 67 publications

References 17 publications

Biobutanol: the outlook of an academic and industrialist

Biobutanol: the outlook of an academic and industrialist

Biobutanol production from rice bran and de-oiled rice bran by Clostridium saccharoperbutylacetonicum N1-4

Production of Acetone, Butanol and Ethanol as Bioenergy Source Materials by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) using Different Substrates

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