Bioconversion of lignocellulose in solid substrate fermentation

Tengerdy, Robert P.; Szakács, George

doi:10.1016/s1369-703x(02)00129-8

Cited by 198 publications

(90 citation statements)

References 74 publications

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“…Xylanases, acting on polymeric xylan chains by hydrolysing the osidic bond between xylose subunits, randomly generate smaller xyloolgiosaccharides [4]. The enzymatic degradation of xylan has numerous industrial and commercial applications, including use in animal feed supplementation, Kraft bleaching, paper manufacturing, municipal waste treatment and in the treatment of lignocellulosic materials for bioethanol production [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris

Gaffney

Carberry

Doyle

et al. 2009

Enzyme and Microbial Technology

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a b s t r a c tA xylanase produced by Thermomyces lanuginosus 195 by solid state fermentation (SSF) was purified 9.3-fold from a crude koji extract, with a 7.6% final yield. The purified xylanase (with an estimated mass of 22 kDa by SDS-PAGE) retained 18% relative activity when treated for 10 min at 100• C and approximately 90% relative activity when incubated at pH values ranging from 6 to 10. Xylanase activity in the purified preparation was significantly enhanced following treatment with manganese and potassium chlorides (p < 0.05) but significantly reduced by calcium, cobalt and iron (p < 0.05). The purified enzyme was also shown to be exclusively xylanolytic. The gene encoding xylanase activity from T. lanuginosus 195 was functionally expressed by Pichia pastoris. MALDI-ToF mass spectrometry and zymography were employed to confirm functional recombinant expression. Maximum xylanase titres were achieved following 120 h induction of the recombinant culture, yielding 26.8 U/mL. Achieving functional protein expression facilitates future efforts to optimise the cultivation conditions for heterologous xylanase production.

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Section: Introductionmentioning

confidence: 99%

Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris

Gaffney

Carberry

Doyle

et al. 2009

Enzyme and Microbial Technology

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“…Studies have showed that it is of great importance that increase of the microbial populations especially the lignocellulose-degrading microorganisms in the compost will help in enhancing lignocellulose-degrading waste decomposition and thus hasten the process of composting with different substrates (Beguin and Aubert, 1994;EL-Din et al, 2000;Hart et al, 2002;Iiyami et al, 1996;Kakezawa et al, 1992;Lu et al, 2004;Straatsma et al, 1994;Tengerdy and Szakacs, 2003). Moreover, agronomical study showed that inoculation of compost with lignocellulose-degrading microorganisms is a potentially successful strategy for improving the product for agronomic purposes (El-Din et al, 2000;Lynch, 1986).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of mesophilic cellulose-degrading bacteria from flower stalks-vegetable waste co-composting system

Wang

Yang

et al. 2005

J. Gen. Appl. Microbiol.

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Fifteen mesophilic bacteria with high C x cellulase activities were isolated and purified from a mixed-culture enriched from a flower stalks-vegetable waste co-composting system. A CMCase test showed that the enzyme activity of these isolates ranged from 7.9 to 28.0 U ml ؊1. Although filter paper degrading capability was low in single culture, significant synergetic cellulose degradation were detected in four groups of mixed cultures, their degradation rates were 23.5%, 26.3%, 19.4% and 24.5%, respectively. Study of morphological and physiological characters of five predominant isolates which possess high CMCase and had positive effect on synergetic cellulose degradation in mixed culture system showed that two of them were closely related to Bacillus pasteurii and Bacillus cereus, whereas the rest belong to the genus Halobacillus, Aeromicrobium and Brevibacterium, respectively.

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“…The effect of adding non-ionic surfactants was proposed to be caused by reduced contact of enzyme with the air-liquid interface due to the surface activity of the surfactant. Eriksson [10] have reported that the addition of non-ionic and anionic surfactants to a hydrolysis mixture of cellulose resulted in a decrement of cellulase adsorption to the residual substrate, which prevents the enzyme from being deactivated by such irrevserble adsorption. On the other hand, this effect of surfactant is able to increase the possibility to recycle enzymes after complete cellulose hydrolysis.…”

Section: Effect Of Surfactants On Enzyme Stabilitymentioning

confidence: 99%

“…However, it takes a long time for the waste to be decomposed completely as the high content of cellulose, which is of a complex structure and hard to degrade [8,9] . The low cellulase activity is another reason why the degradation of cellulose is slow [10] . Cellulase, which catalyses the hydrolysis of cellulose to D-glucose, is dependent on the types of cellulolytic microorganisms present in the compost matrix [11] .…”

Section: Introductionmentioning

confidence: 99%

Hydrolysis of pretreated rice straw with surfactants at low cellulase dosage

Liang¹,

Huang²,

Miao³

et al. 2016

Proceedings of the 2016 5th International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2016)

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Abstract:The effects of three surfactants (Tween 80, saponin and monorhamnolipid) on the hydrolysis of alkali pretreated rice straw by low dosage of cellulase were studied. The results indicated that with a relatively low cellulase dosage (4 FPU g -1 substrate), all surfactants were able to enhance the enzymatic hydrolysis, while the biosurfactant monorhamnolipid was demonstrated to be more active than Tween 80 and saponin. Monorhamnolipid at 0.006% increased reducing sugar yield by 23.15%, and the maximal sugar yield was obtained within half of the time compare to the control required. The cellulase losed 67% of its activity in the control, while the activity lost ratios were 31%~43% in the presence of the surfactants. The surfactants addition did not affect the pH of the hydrolysis system. This study provides an opportunity of decreasing enzyme dosage in hydrolysis of agricultural solid waste rich in cellulose.

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Bioconversion of lignocellulose in solid substrate fermentation

Cited by 198 publications

References 74 publications

Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris

Purification and characterisation of a xylanase from Thermomyces lanuginosus and its functional expression by Pichia pastoris

Isolation and characterization of mesophilic cellulose-degrading bacteria from flower stalks-vegetable waste co-composting system

Hydrolysis of pretreated rice straw with surfactants at low cellulase dosage

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