Bioconversion of lignocellulosic ‘waste’ to high‐value food proteins: Recombinant production of bovine and human α_S1‐casein based on wheat straw lignocellulose

Abstract

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Fungal Cell Factories for Efficient and Sustainable Production of Proteins and Peptides

“…A major challenge in replacing animal products with animal proteins produced in microbes on a commercial scale is to ensure that the processes can be scaled up in a profitable way, as the food proteins have less commercial value compared to enzymes and pharmaceutical products in the fermentation industry. To keep production costs low, production could be carried out in biorefineries with lignocellulosic plant material, as shown by Wang et al [123], who produced recombinant bovine and human αS1-casein using a hydrolysate from wheat straw lignocellulose.…”

“…In principle, sidestreams from various agricultural processes can be used as nutritious substrates for microbial production [168]. However, many sidestreams are solid and consist of lignocellulose, which require homogenizing, strong pre-treatment, and enzymatic hydrolysis, before they can be applied as hydrolysates for SmF [123]. However, plant juices from various industrial food production sources with readily available sugar content can be easily applied to SmF without heavy energy-intensive pre-treatments.…”

“…Solid sidestreams from local production of cereals, maize, oilseed rape, sugar and fodder beet, potato, grass, and legumes can be applied for SSF processes [81,84,85,163], instead of the harsh pre-treatment to produce readily available fermentable sugars for SmF [123]. Many of these sidestreams are currently underutilized or used as low-value feed or for biogas.…”