Bioconversion of municipal solid waste to glucose for bio-ethanol production

Abstract

Selected biodegradable municipal solid waste fractions were subjected to fifteen different pre-hydrolysis treatments to obtain the highest glucose yield for bio-ethanol production. Pre-hydrolysis treatments consisted of dilute acid (H(2)SO(4), HNO(3) or HCl, 1 and 4%, 180 min, 60 degrees C), steam treatment (121 and 134 degrees C, 15 min), microwave treatment (700 W, 2 min) or a combination of two of them. Enzymatic hydrolysis was carried out with Trichoderma reesei and Trichoderma viride (10 and 60 FPU g(-1) substrate). Glucose yields were compared using a factorial experimental design. The highest glucose yield (72.80%) was obtained with a pre-hydrolysis treatment consisting of H(2)SO(4) at 1% concentration, followed by steam treatment at 121 degrees C, and enzymatic hydrolysis with Trichoderma viride at 60 FPU g(-1) substrate. The contribution of enzyme loading and acid concentration was significantly higher (49.39 and 47.70%, respectively), than the contribution of temperature during steam treatment (0.13%) to the glucose yield.

Bioremediation and biovalorisation of olive-mill wastes

“…Additionally, the high content of organic matter makes OMWs an interesting alternative resource to produce ethanol as a biofuel (Li et al 2007). Even if the content of free reducing sugar in these wastes is low, different kinds of polysaccharides can be converted to ethanol via different reactions that occur in two separate steps: first an enzymatic hydrolysis using commercial enzymes followed by the conversion of reducing sugars to ethanol performed by yeasts (alcoholic fermentation; Zanichelli et al 2007).…”

Microbial Biotechnology in Olive Oil Industry

“…Ethanol production as a biofuel from OMWs with high content of organic matter is interesting (Li et al 2007). The two main components of TPOMW (stones and olive pulp) as substrates were used to production of ethanol by a simultaneous saccharification and fermentation process (Ballesteros et al 2001).…”

Second‐generation biofuels and local bioenergy systems

“…6,13 Th e biofuel industry could also benefi t from other biomass feedstock, such as solid waste including green waste, food waste and biodegradable fractions of municipal solid waste (MSW). 17 Cellulose and polymeric hemicelluloses (mainly xylans) are the main components that constitute these lignocellulosic materials, and their bioconversion require a pre-treatment process. 18,19 Th e pre-treatment and hydrolysis of lignocelluloses can be carried out physically (e.g., steam treatment), chemically (by acid or alkaline hydrolysis) and enzymatically (using cellulases, hemicellulases and ligninases from various fungi), or using a combination of these methods.…”