Biodegradable and adjustable sol-gel glass based hybrid scaffolds from multi-armed oligomeric building blocks

Kascholke, Christian; Hendrikx, Stephan; Flath, Tobias; Kuzmenka, Dzmitry; Dörfler, Hans-Martin; Schümann, Dirk; Gressenbuch, Mathias; Schulze, Fritz Peter; Schulz‐Siegmund, Michaela; Hacker, Michael C.

doi:10.1016/j.actbio.2017.09.024

Cited by 16 publications

(14 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, chondrogenic differentiation of human bone marrow-derived MSCs in 3D silica fabrics was investigated. For the chondrogenic differentiation of MSCs, we utilized 3D silica fabrics as previously reported, as it is a reasonable scaffold for tissue engineering [34][35][36]38]. Briefly, 3D silica fabrics were developed via the sol-gel process to create highly interconnected porous microstructures of electrospun fabrics for cell growth.…”

Section: Discussionmentioning

confidence: 99%

“…Silica-based hybrid materials have been widely used as implantable materials for cartilage and bone tissue regeneration. For example, Kascholke et al developed biodegradable and adjustable sol-gel glass-based hybrid scaffolds for cartilage and bone tissue regeneration [36]. This scaffold was biodegradable, and MSCs embedded in the scaffold proliferated and displayed promoted cell function.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cartilage Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Three-Dimensional Silica Nonwoven Fabrics

et al. 2018

View full text Add to dashboard Cite

In cartilage tissue engineering, three-dimensional (3D) scaffolds provide native extracellular matrix (ECM) environments that induce tissue ingrowth and ECM deposition for in vitro and in vivo tissue regeneration. In this report, we investigated 3D silica nonwoven fabrics (Cellbed®) as a scaffold for mesenchymal stem cells (MSCs) in cartilage tissue engineering applications. The unique, highly porous microstructure of 3D silica fabrics allows for immediate cell infiltration for tissue repair and orientation of cell–cell interaction. It is expected that the morphological similarity of silica fibers to that of fibrillar ECM contributes to the functionalization of cells. Human bone marrow-derived MSCs were cultured in 3D silica fabrics, and chondrogenic differentiation was induced by culture in chondrogenic differentiation medium. The characteristics of chondrogenic differentiation including cellular growth, ECM deposition of glycosaminoglycan and collagen, and gene expression were evaluated. Because of the highly interconnected network structure, stiffness, and permeability of the 3D silica fabrics, the level of chondrogenesis observed in MSCs seeded within was comparable to that observed in MSCs maintained on atelocollagen gels, which are widely used to study the chondrogenesis of MSCs in vitro and in vivo. These results indicated that 3D silica nonwoven fabrics are a promising scaffold for the regeneration of articular cartilage defects using MSCs, showing the particular importance of high elasticity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cartilage Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Three-Dimensional Silica Nonwoven Fabrics

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Multi-armed oligomers composed of an ethoxylated alcohol core and hydrolytically degradable oligo( d , l -lactide) units were used as the organic component of a hybrid. Different degrees of ethoxylation and varying length and content of oligoester units and varying content of organic component were used as tools to control biodegradation and mechanical properties of the hybrid [160]. The 2- to 4-armed organic crosslinkers with small organic core molecules functionalized with ICPTES were synthesized to crosslink a pure silica sol.…”

Section: Bone Tissue Engineering Scaffolds Encompass the Chemistrymentioning

confidence: 99%

Bone Repair and Regenerative Biomaterials: Towards Recapitulating the Microenvironment

et al. 2019

View full text Add to dashboard Cite

Biomaterials and tissue engineering scaffolds play a central role to repair bone defects. Although ceramic derivatives have been historically used to repair bone, hybrid materials have emerged as viable alternatives. The rationale for hybrid bone biomaterials is to recapitulate the native bone composition to which these materials are intended to replace. In addition to the mechanical and dimensional stability, bone repair scaffolds are needed to provide suitable microenvironments for cells. Therefore, scaffolds serve more than a mere structural template suggesting a need for better and interactive biomaterials. In this review article, we aim to provide a summary of the current materials used in bone tissue engineering. Due to the ever-increasing scientific publications on this topic, this review cannot be exhaustive; however, we attempted to provide readers with the latest advance without being redundant. Furthermore, every attempt is made to ensure that seminal works and significant research findings are included, with minimal bias. After a concise review of crystalline calcium phosphates and non-crystalline bioactive glasses, the remaining sections of the manuscript are focused on organic-inorganic hybrid materials.

show abstract

“…Recently, the scaffolds fabricated by 3D-printing have been utilized for application in bone tissue engineering. 18 The significant advantages of this method are that the porous structures (pore morphology, pore size, porosity and interconnectivity, etc. ) of the scaffolds can be concisely controlled by layer-by-layer plotting under different conditions.…”

Section: Introductionmentioning

confidence: 99%

“…of the scaffolds can be concisely controlled by layer-by-layer plotting under different conditions. 18,19 Therefore, in this study, nano magnesium silicate (n-MS) particles were fabricated, and bioactive and degradable scaffolds of n-MS/ZN/PCL ternary composites with controllable pore structure were developed by 3D printing method. The objective of this study to investigate the effects of ZN content in the scaffolds on in vitro apatite mineralization, degradability, cell responses and in vivo osteogenesis properties of the ternary composites scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Zein regulating apatite mineralization, degradability, in vitro cells responses and in vivo osteogenesis of 3D-printed scaffold of n-MS/ZN/PCL ternary composite

Wei

Yang

et al. 2018

RSC Adv.

View full text Add to dashboard Cite

Bioactive and degradable scaffolds of nano magnesium silicate (n-MS)/zein (ZN)/poly(caprolactone) (PCL) ternary composites were prepared by 3D-printing method. The results showed that the 3D-printed scaffolds possessed controllable pore structure, and pore morphology, pore size, porosity and pore interconnectivity of the scaffolds can be efficiently adjusted. In addition, the apatite-mineralization ability of the scaffolds in simulated body fluids was obviously improved with the increase of ZN content, in which the scaffold with 20 w% ZN (C20) possessed excellent apatite-mineralization ability. Moreover, the degradability of the scaffolds was significantly enhanced with the increase of ZN content in the scaffolds.The degradation of ZN produced acidic products that could neutralize the alkaline products from the degradation of n-MS, which avoid the increase of pH value in degradable solution. Furthermore, the MC3T3-E1 cells responses (e.g. proliferation and differentiation, etc.) to the scaffolds were significantly promoted with the increase of ZN content. The in vivo osteogenesis of the scaffolds implanted the femur defects of rabbits was investigated by micro-CT and histological analysis. The results demonstrated that the new bone formation was significantly enhanced with the increase of ZN content, in which the C20 scaffold induced the highest new bone tissues, indicating excellent osteogenesis. The results suggested that the ZN in the ternary composite scaffolds played key roles in assisting bone regeneration in vivo. Fig. 1 TEM image (a) and EDS (b) of n-MS, and XRD (c) of n-MS and ZN, and XRD (d) of C0, C10 and C20 scaffolds.Fig. 3 SEM micrographs of C0 (a and b), C10 (c and d) and C20 (e and f) scaffolds under different magnification after immersed into SBF for 5 days, EDS (g) of C20 scaffold immersed into SBF for 5 days, and the change of ions concentrations with time (h) after C20 scaffold immersed into SBF.

show abstract

Biodegradable and adjustable sol-gel glass based hybrid scaffolds from multi-armed oligomeric building blocks

Cited by 16 publications

References 60 publications

Cartilage Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Three-Dimensional Silica Nonwoven Fabrics

Cartilage Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Three-Dimensional Silica Nonwoven Fabrics

Bone Repair and Regenerative Biomaterials: Towards Recapitulating the Microenvironment

Zein regulating apatite mineralization, degradability, in vitro cells responses and in vivo osteogenesis of 3D-printed scaffold of n-MS/ZN/PCL ternary composite

Contact Info

Product

Resources

About