Biodegradable fibrous scaffolds with diverse properties by electrospinning candidates from a combinatorial macromer library

Metter, Robert B.; Ifkovits, Jamie L.; Hou, Kevin; Vincent, L.; Hsu, Benjamin; Wang, Louis; Mauck, Robert L.; Burdick, Jason A.

doi:10.1016/j.actbio.2009.10.027

Cited by 22 publications

(18 citation statements)

References 36 publications

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“…One recently developed such family of materials are poly(b-amino ester)s (PBAEs), which have been investigated for gene 12,13 and drug [14][15][16][17][18][19][20][21] delivery, and tissue engineering. [22][23][24][25][26][27] They show pH sensitivity in addition to being biodegradable and highly biocompatible. PBAEs are easily synthesized by Michael addition of bifunctional amines to diacrylates with no byproducts.…”

Section: Introductionmentioning

confidence: 99%

Bisphosphonate‐functionalized poly(β‐amino ester) network polymers

Guven

Altuncu

Duman

et al. 2017

J Biomedical Materials Res

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Three novel bisphosphonate-functionalized secondary diamines are synthesized and incorporated into poly(β-amino ester)s (PBAEs) to investigate the effects of bisphosphonates on biodegradation and toxicity of PBAE polymer networks. These three novel amines, BPA1, BPA2, and BPA3, were prepared from the reactions of 1,4-butanediamine, 1,6-hexanediamine, or 4,9-dioxa-1,12-dodecanediamine with tetraethyl vinylidene bisphosphonate, respectively. The PBAE macromers were obtained from the aza-Michael addition reaction of these amines to 1,6-hexane diol diacrylate (HDDA) and poly(ethylene glycol) diacrylate (PEGDA, M = 575) and photopolymerized to produce biodegradable gels. These gels with different chemistries exhibited similar degradation behavior with mass loss of 53-73% within 24 h, indicating that degradation is mostly governed by the bisphosphonate group. Based on the in vitro cytotoxicity evaluation against NIH 3T3 mouse embryonic fibroblast cells, the degradation products do not exhibit significant toxicity in most cases. It was also shown that PBAE macromers can be used as cross-linkers for the synthesis of 2-hydroxyethyl methacrylate hydrogels, conferring small and customizable degradation rates upon them. The materials reported have potential to be used as nontoxic degradable biomaterials. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1412-1421, 2017.

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Section: Introductionmentioning

confidence: 99%

Bisphosphonate‐functionalized poly(β‐amino ester) network polymers

Guven

Altuncu

Duman

et al. 2017

J Biomedical Materials Res

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“…Moreover, the electrospinning process is also compatible with photopolymerizable materials [4], [11], [12], [13], with the introduction of an initiator to the electrospinning solution and then light after fiber formation. Fibers are aligned in the electrospinning process by collection onto a rotating mandrel [14], [15], [16], [17], [18].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Fibrous Elastomer Structure and Porosity on Matrix Organization

Ifkovits

Mauck

et al. 2010

PLoS ONE

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Fibrous scaffolds are finding wide use in the field of tissue engineering, as they can be designed to mimic many native tissue properties and structures (e.g., cardiac tissue, meniscus). The influence of fiber alignment and scaffold architecture on cellular interactions and matrix organization was the focus of this study. Three scaffolds were fabricated from the photocrosslinkable elastomer poly(glycerol sebacate) (PGS), with changes in fiber alignment (non-aligned (NA) versus aligned (AL)) and the introduction of a PEO sacrificial polymer population to the AL scaffold (composite (CO)). PEO removal led to an increase in scaffold porosity and maintenance of scaffold anisotropy, as evident through visualization, mechanical testing, and mass loss studies. Hydrated scaffolds possessed moduli that ranged between ∼3–240 kPa, failing within the range of properties (<300 kPa) appropriate for soft tissue engineering. CO scaffolds were completely degraded as early as 16 days, whereas NA and AL scaffolds had ∼90% mass loss after 21 days when monitored in vitro. Neonatal cardiomyocytes, used as a representative cell type, that were seeded onto the scaffolds maintained their viability and aligned along the surface of the AL and CO fibers. When implanted subcutaneously in rats, a model that is commonly used to investigate in vivo tissue responses to biomaterials, CO scaffolds were completely integrated at 2 weeks, whereas ∼13% and ∼16% of the NA and AL scaffolds, respectively remained acellular. However, all scaffolds were completely populated with cells at 4 weeks post-implantation. Polarized light microscopy was used to evaluate the collagen elaboration and orientation within the scaffold. An increase in the amount of collagen was observed for CO scaffolds and enhanced alignment of the nascent collagen was observed for AL and CO scaffolds compared to NA scaffolds. Thus, these results indicate that the scaffold architecture and porosity are important considerations in controlling tissue formation.

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“…These are perhaps the most prominent examples, although many other synthetic materials are currently being pursued. Additionally, emerging synthetic polymers taken from combinatorial libraries, including poly(arylates) and poly(b-amino esters) (PBAEs) [11][12][13], indicate that the list of degradable synthetic polymers is rapidly expanding, many of which will be translated to the electrospun format [12]. The key processing features of and advantages/drawbacks of several of these polymer systems will be discussed in the following subsections.…”

Section: Synthetic Polymersmentioning

confidence: 99%

Synthetic/Biopolymer Nanofibrous Composites as Dynamic Tissue Engineering Scaffolds

Kluge

Mauck

2011

Biomedical Applications of Polymeric Nanofibers

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Synthetic/biopolymer composite scaffolds can provide improved flexibility when designing optimized stem-cell-laden tissue repair devices for fiberreinforced tissues. Composites enable a range of mechanical properties, rates of degradation, and other functional attributes of a degradable scaffold to be finely tuned by modifying polymer sources and their processing techniques. Furthermore, marrying synthetic fibers spun from harsh solvents with polymers spun from mild organic or aqueous-based solvents can allow for delivery of active molecular species in these composites. In the pre-processing fabrication steps, polymers can be selected and combined from traditional or newer high-throughput approaches into networks with properties that reflect the individual components or their interactions. These properties may also depend on the spinning solvents used. In post-processing steps, factors such as the ambient environmental conditions or crosslinking solvent treatments can differentially affect the constituent polymers chosen. Several case studies will be discussed in order to highlight the potential advantages of these approaches. For example, many studies have shown that the mechanical properties of composite mats can be designed with superior or more tissue-like properties than individual polymer sources alone. Further, it has been shown that the degradation rates of the temporary scaffold can be more finely tuned in composites by combining rapidly and slowly degrading systems. The drug and growth factor delivery capabilities of these systems will similarly be reviewed. We conclude with an overview of several tissue engineering approaches that have exploited composite design features and discuss new promising avenues for study.

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Biodegradable fibrous scaffolds with diverse properties by electrospinning candidates from a combinatorial macromer library

Cited by 22 publications

References 36 publications

Bisphosphonate‐functionalized poly(β‐amino ester) network polymers

Bisphosphonate‐functionalized poly(β‐amino ester) network polymers

The Influence of Fibrous Elastomer Structure and Porosity on Matrix Organization

Synthetic/Biopolymer Nanofibrous Composites as Dynamic Tissue Engineering Scaffolds

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