Biodegradable guide for bone regeneration

Nielsen, Frank Farsø; Karring, Thorkild; Gogolewski, Sylwester

doi:10.3109/17453679209154853

Cited by 66 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current and previous studies, we describe the observed early, woven bone proliferation as intramembranous rather than endochondral [21], [22], [39], which better fits with Albrektsson and Johansson's definition. Taken one step further, we consider the relative barrier properties of the membrane sleeve, in conjunction with the impermeable surface of the intramedullary nail, to provide a conduit that guides bone regeneration inwards radially, similar (but in an opposite direction) to that suggested by Gogolewski and colleagues [40]. Finally, a gradient of cells and osteogenic factors is expected to develop between the inner surface of the directional delivery membrane and the outer surface of the IM nail, as well as between the edges and center of the defect zone; cellular and biochemical gradients can further facilitate osteogenesis via osteoinduction and osteoconduction.…”

Section: Discussionmentioning

confidence: 99%

Surgical Membranes as Directional Delivery Devices to Generate Tissue: Testing in an Ovine Critical Sized Defect Model

et al. 2011

View full text Add to dashboard Cite

PurposePluripotent cells residing in the periosteum, a bi-layered membrane enveloping all bones, exhibit a remarkable regenerative capacity to fill in critical sized defects of the ovine femur within two weeks of treatment. Harnessing the regenerative power of the periosteum appears to be limited only by the amount of healthy periosteum available. Here we use a substitute periosteum, a delivery device cum implant, to test the hypothesis that directional delivery of endogenous periosteal factors enhances bone defect healing.MethodsNewly adapted surgical protocols were used to create critical sized, middiaphyseal femur defects in four groups of five skeletally mature Swiss alpine sheep. Each group was treated using a periosteum substitute for the controlled addition of periosteal factors including the presence of collagen in the periosteum (Group 1), periosteum derived cells (Group 2), and autogenic periosteal strips (Group 3). Control group animals were treated with an isotropic elastomer membrane alone. We hypothesized that periosteal substitute membranes incorporating the most periosteal factors would show superior defect infilling compared to substitute membranes integrating fewer factors (i.e. Group 3>Group 2>Group 1>Control).ResultsBased on micro-computed tomography data, bone defects enveloped by substitute periosteum enabling directional delivery of periosteal factors exhibit superior bony bridging compared to those sheathed with isotropic membrane controls (Group 3>Group 2>Group 1, Control). Quantitative histological analysis shows significantly increased de novo tissue generation with delivery of periosteal factors, compared to the substitute periosteum containing a collagen membrane alone (Group 1) as well as compared to the isotropic control membrane. Greatest tissue generation and maximal defect bridging was observed when autologous periosteal transplant strips were included in the periosteum substitute.ConclusionPeriosteum-derived cells as well as other factors intrinsic to periosteum play a key role for infilling of critical sized defects.

show abstract

Section: Discussionmentioning

confidence: 99%

Surgical Membranes as Directional Delivery Devices to Generate Tissue: Testing in an Ovine Critical Sized Defect Model

et al. 2011

View full text Add to dashboard Cite

show abstract

“…16,17 The biopolymers have been tested for application in orthopedic and dental surgery. 8,18 In this study, the biocompatibility of three different chemical compositions of RCP was evaluated. The present results showed that all implants, independent of the RCP composition, were biocompatible, as demonstrated by the quality of the RCP-tissue interface and by the capacity for osseointegration.…”

Section: Discussionmentioning

confidence: 99%

In vivo biocompatibility of three different chemical compositions of Ricinus communis polyurethane

Barros

Beloti

Chierice

2003

J Biomedical Materials Res

View full text Add to dashboard Cite

Alteration in the chemical composition of a biomaterial may be undertaken to improve its biological properties. The aim of this work was to evaluate the biocompatibility of three chemical compositions of Ricinus communis polyurethane (RCP): RCPp (pure RCP), RCP + CaCO(3), and RCP +Ca(3)(PO(4))(2). RCP cylinders were surgically implanted in rabbit femurs. After 8, 12, and 16 weeks, the femurs were removed, fixed, sectioned, ground, and stained by Stevenel's blue/Alizarin red S for light microscopy and histomorphometry. The osseointegration and osseoconductivity were calculated by means of image analysis and the data were submitted to the Kruskal-Wallis test followed by Dunn's test. Osseointegration was already completed after 8 weeks on RCP + Ca(3)(PO(4))(2) because similar values were found from week 8 to 16, whereas it showed a time-dependent increase on RCPp and RCP +CaCO(3). The osseointegration was greater on RCP + Ca(3)(PO(4))(2) in all periods when compared with RCPp, and after 8 and 12 weeks when compared with RCP + CaCO(3). None of the RCP samples presented osseoconductivity. The present results showed that RCP blended with calcium phosphate improved the biocompatibility by both enhancing and accelerating its osseointegration. Based on the absence of osseoconductivity, RCP was considered to be a bioinert material.

show abstract

“…[16] Moreover, the requirement for organic solvents during processing prevents cell or protein encapsulation. [17] Synthetic bioelastomers based on polyurethanes modified with degradable segments have also been developed [5] and used for soft tissue, [18] bone, [19] and myocardial [20] repairs. While these materials have shown promise, concerns remain over potential toxicity of degradation products and the ability of these materials to resist long term strains in vivo .…”

Section: Introductionmentioning

confidence: 99%

Highly Tunable Elastomeric Silk Biomaterials

Partlow

Hanna

Rnjak‐Kovacina

et al. 2014

Adv Funct Materials

365

443

View full text Add to dashboard Cite

Elastomeric, fully degradable and biocompatible biomaterials are rare, with current options presenting significant limitations in terms of ease of functionalization and tunable mechanical and degradation properties. We report a new method for covalently crosslinking tyrosine residues in silk proteins, via horseradish peroxidase and hydrogen peroxide, to generate highly elastic hydrogels with tunable properties. The tunable mechanical properties, gelation kinetics and swelling properties of these new protein polymers, in addition to their ability to withstand shear strains on the order of 100%, compressive strains greater than 70% and display stiffness between 200 – 10,000 Pa, covering a significant portion of the properties of native soft tissues. Molecular weight and solvent composition allowed control of material mechanical properties over several orders of magnitude while maintaining high resilience and resistance to fatigue. Encapsulation of human bone marrow derived mesenchymal stem cells (hMSC) showed long term survival and exhibited cell-matrix interactions reflective of both silk concentration and gelation conditions. Further biocompatibility of these materials were demonstrated with in vivo evaluation. These new protein-based elastomeric and degradable hydrogels represent an exciting new biomaterials option, with a unique combination of properties, for tissue engineering and regenerative medicine.

show abstract

Biodegradable guide for bone regeneration

Cited by 66 publications

References 20 publications

Surgical Membranes as Directional Delivery Devices to Generate Tissue: Testing in an Ovine Critical Sized Defect Model

Surgical Membranes as Directional Delivery Devices to Generate Tissue: Testing in an Ovine Critical Sized Defect Model

In vivo biocompatibility of three different chemical compositions of Ricinus communis polyurethane

Highly Tunable Elastomeric Silk Biomaterials

Contact Info

Product

Resources

About